Results 31 to 40 of about 2,576,180 (238)

Cassettes for PCR-mediated gene tagging in Candida albicans utilizing nourseothricin resistance [PDF]

, 2011
In recent years a number of molecular tools have been reported for use in the human fungal pathogen Candida albicans, including PCR-mediated approaches for gene disruption, conditional expression and epitope tagging.
Jill Cheetham (21825818), Stephen Aves (21816917), SW Milne (21825815), D Lloyd (7797887), S Bates (13319511)   +4 more
core   +6 more sources

Characterization Integrons and Gene Cassettes in Trimethoprim/Sulfamethoxazole-resistant Stenotrophomonas maltophilia

Journal of Medical Sciences
Background: Integrons in Stenotrophomonas maltophilia are the major mechanism for trimethoprim/sulfamethoxazole (TMP/SMX) resistance. Molecular epidemiology of S.
Jiun-Ji Lai, Yi-Ping Chuang, Rui-Xin Wu, Ching-Mei Yu, Hung-Sheng Shang, Ching-Hsun Wang   +5 more
doaj   +1 more source

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Phylogenetic Profiler for Gene Cassettes.

, 2013
Phylogenetic Profiler for Gene Cassettes.
Nikos C. Kyrpides (13837), Konstantinos Mavromatis (13836), Natalia Ivanova (8850), Sean D. Hooper (5540), Ken Chu (283940), Victor M. Markowitz (13826)   +5 more
core   +1 more source

Inverse correlation between promoter strength and excision activity in class 1 integrons. [PDF]

PLoS Genetics, 2010
Class 1 integrons are widespread genetic elements that allow bacteria to capture and express gene cassettes that are usually promoterless. These integrons play a major role in the dissemination of antibiotic resistance among Gram-negative bacteria.
Thomas Jové, Sandra Da Re, François Denis, Didier Mazel, Marie-Cécile Ploy   +4 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Emergence of the mcr-1 colistin resistance gene in extended-spectrum β-lactamase-producing Klebsiella pneumoniae in Taiwan

Journal of Global Antimicrobial Resistance, 2021
Objectives: The resistance ofmcr-1-carrying Enterobacteriaceae to polymyxins, which are last-resort antibiotics, has raised great concern worldwide. In this study, four mcr-1-carrying plasmids isolated from Klebsiella pneumoniae clinical isolates were ...
Yu-Chi Lin, Makoto Kuroda, Satowa Suzuki, Jung-Jung Mu   +3 more
doaj   +1 more source

Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research

FEBS Letters, EarlyView.
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

Characteriz ation of integrons and associated gene cassettes in Acinetobacter baumannii strains isolated from intensive care unit in Tehran, Iran

Journal of Acute Disease, 2016
Objective: To determine the antimicrobial susceptibility patterns, the frequency of integrons and associated gene cassettes in Acinetobacter baumannii (A. baumannii) strains isolated from selected hospital intensive care units.
Hossein Goudarzi, Mehdi Azad, Sima Sadat Seyedjavadi, Hadi Azimi, Alireza Salimi Chirani, Vahid Fallah Omrani, Mehdi Goudarzi   +6 more
doaj   +1 more source