Detection of Novel Integrons in the Metagenome of Human Saliva.
PLoS ONE, 2016 Integrons are genetic elements capable of capturing and expressing open reading frames (ORFs) embedded within gene cassettes. They are involved in the dissemination of antibiotic resistance genes (ARGs) in clinically important pathogens.
Supathep Tansirichaiya +4 more
doaj +1 more source
Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination [PDF]
, 2013 Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase.
Colloms, S.D. +7 more
core +4 more sources
A novel expression cassette for the efficient visual selection of transformed tissues in florists' chrysanthemum (Chrysanthemum morifolium Ramat.). [PDF]
, 2011 Constructs carrying visual reporter genes coupled with efficient promoters could facilitate the process of identification and selection of stable transformants in recalcitrant crops.
Jongsma, M.A. +3 more
core +2 more sources
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes. [PDF]
J Vis Exp, 2015 Antibiotic resistance is one of the greatest threats to health in the 21st century. Acquisition of resistance genes via lateral gene transfer is a major factor in the spread of diverse resistance mechanisms. Amongst the DNA elements facilitating lateral transfer, the class 1 integrons have largely been responsible for spreading antibiotic resistance ...
Waldron LS, Gillings MR.
europepmc +4 more sources
Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana. [PDF]
, 2016 To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-
Alkanaimsh, Salem +13 more
core +1 more source
mBio, 2016 Class 1 integrons are genetic systems that enable bacteria to capture and express gene cassettes. These integrons, when isolated in clinical contexts, most often carry antibiotic resistance gene cassettes.
Emilie Strugeon +3 more
doaj +1 more source
Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
core +1 more source
Dynamics of class 1 integrons in aerobic biofilm reactors spiked with antibiotics
Environment International, 2020 Class 1 integrons are strongly associated with the dissemination of antibiotic resistance in bacteria. However, little is known about whether the presence of antibiotics affects the abundance of integrons and antibiotic resistance genes during biological
Jiaoqi Huyan +6 more
doaj +1 more source
Cassettes for PCR-mediated gene tagging in Candida albicans utilizing nourseothricin resistance [PDF]
, 2013 addresses: Biosciences, College of Life and Environmental Sciences, University of Exeter, UK.types: Journal Article; Research Support, Non-U.S. Gov't; Validation StudiesThis is the author's post-print version of an article published in Yeast, 2011, Vol ...
Bain +37 more
core +1 more source
Context-driven discovery of gene cassettes in mobile integrons using a computational grammar
BMC Bioinformatics, 2009 Background Gene discovery algorithms typically examine sequence data for low level patterns. A novel method to computationally discover higher order DNA structures is presented, using a context sensitive grammar.
Schaeffer Jaron +4 more
doaj +1 more source