Results 11 to 20 of about 1,118,644 (205)

Plant gene editing improved [PDF]

Nature Reviews Molecular Cell Biology, 2019
The tissue culture bottleneck for gene editing in plants can be overcome by modifying somatic cells in whole plants and inducing the formation of shoot meristems.
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Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy [PDF]

, 2018
Mutations in the gene encoding dystrophin, a protein that maintains muscle integrity and function, cause Duchenne muscular dystrophy (DMD). The deltaE50-MD dog model of DMD harbors a mutation corresponding to a mutational “hotspot” in the human DMD gene.
Amoasii, L, Bassel-Duby, R, Caballero, D, Harron, R, Hildyard, J C W, Li, H, Massey, C A, Mireault, A, Olson, E N, Piercy, R J, Sanchez-Ortiz, E, Shelton, J M, Stathopoulou, T-R   +12 more
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Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. [PDF]

, 2017
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase,
Bai, Haipeng, Huang, Haigen, Li, Song, Lin, Shuo, Lu, Xiaochan, Qin, Wei, Xu, Jason, Zhang, Yihan   +7 more
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Efficient generation of brain organoids using magnetized gold nanoparticles

Scientific Reports, 2023
Brain organoids, which are three-dimensional cell culture models, have the ability to mimic certain structural and functional aspects of the human brain.
Hongwon Kim, Yoo-Jung Lee, Youngeun Kwon, Jongpil Kim   +3 more
doaj   +1 more source

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo. [PDF]

, 2018
Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5 end can enhance the gene editing efficiency of Cpf1 both in cells
Chong, Anthony, Chu, Hunghao, Fellmann, Christof, Jiang, Fuguo, Lee, Kunwoo, Liu, Hui, Mackley, Vanessa, Murthy, Niren, Park, Hyo, Rao, Anirudh, Wu, Joann   +10 more
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APOE ε4-dependent effects on the early amyloid pathology in induced neurons of patients with Alzheimer’s disease

Translational Neurodegeneration, 2022
Background The ε4 allele of apolipoprotein E (APOE ε4) is the strongest known genetic risk factor for late-onset Alzheimer’s disease (AD), associated with amyloid pathogenesis. However, it is not clear how APOE ε4 accelerates amyloid-beta (Aβ) deposition
Hongwon Kim, Siyoung Kim, Byounggook Cho, Jaein Shin, Jongpil Kim   +4 more
doaj   +1 more source

Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells. [PDF]

, 2019
Repurposed CRISPR-Cas molecules provide a useful tool set for broad applications of genomic editing and regulation of gene expression in prokaryotes and eukaryotes. Recent discovery of phage-derived proteins, anti-CRISPRs, which serve to abrogate natural
Abbott, Timothy R, Bondy-Denomy, Joseph, Carter, Matthew A, Chavez, Michael, Dominguez, Antonia A, Gao, Yuchen, Kipniss, Nathan H, La Russa, Marie, Lau, Matthew B, Nakamura, Muneaki, Qi, Lei S, Smolke, Christina D, Srinivasan, Prashanth, Xu, Xiaoshu, Zhao, Dehua   +14 more
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CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease

Translational Neurodegeneration, 2022
Background Aberrant DNA methylation patterns have been observed in neurodegenerative diseases, including Alzheimer's disease (AD), and dynamic changes in DNA methylation are closely associated with the onset and progression of these diseases ...
Hanseul Park, Jaein Shin, Yunkyung Kim, Takashi Saito, Takaomi C. Saido, Jongpil Kim   +5 more
doaj   +1 more source

Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41

mBio, 2022
Previous studies suggest that short peptides from the heptad repeat 2 (HR2) domain of gp41 expressed on the cell surface are more potent inhibitors of HIV-1 entry than soluble analogs.
Alexandra Maslennikova, Natalia Kruglova, Svetlana Kalinichenko, Dmitriy Komkov, Mikhail Shepelev, Dmitriy Golubev, Andrei Siniavin, Andrei Vzorov, Alexander Filatov, Dmitriy Mazurov   +9 more
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Ethics in Gene Editing

Open Access Indonesian Journal of Medical Reviews, 2021
For thousands of years, humans have felt the need to understand the world around them—and ultimately manipulate it to best serve their needs. There are always ethical questions to address, especially when the manipulation involves the human genome. There is currently an urgent need to actively pursue those conversations as com- mercial gene sequencing ...
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