Results 21 to 30 of about 7,473,287 (361)
Mutations of MSH5 in nonobstructive azoospermia (NOA) and rescued via in vivo gene editing
Signal Transduction and Targeted Therapy, 2022 Dear Editor, Male infertility is a multifactorial heterogeneous pathological condition affecting ∼7% of men. Nonobstructive azoospermia (NOA) is one of the most severe male reproductive diseases and occurs in ∼1% of men of reproductive age.
Min Chen +11 more
semanticscholar +1 more source
Gene editing and gene regulation with CRISPR [PDF]
Experimental Physiology, 2018 Genome editing enables precise changes to be made in the genome of living cells. The technique was originally developed in the 1980’s but largely limited to use in mice. The discovery that a targeted double stranded break (DSB) at a unique site in the genome, close to the site to be changed, could substantially increase the efficiency of editing ...
Patrick Harrison, Stephen Hart
openaire +4 more sources
Nature Materials, 2021 Endosomal escape remains a fundamental barrier hindering the advancement of nucleic acid therapeutics. Taking inspiration from natural phospholipids that comprise biological membranes, we report the combinatorial synthesis of multi-tailed ionizable ...
Shuai Liu +6 more
semanticscholar +1 more source
Tumor evolution selectively inactivates the core microRNA machinery for immune evasion
Nature Communications, 2021 Dysregulation of the microRNA machinery has crucial roles in cancer development. Here the authors show that inactivation of proteins involved in microRNA-mediated gene silencing, such as ANKRD52 or AGO2, confers resistance to T cell-mediated immune ...
Tian-Yu Song +15 more
doaj +1 more source
Gene Editing by Extracellular Vesicles [PDF]
International Journal of Molecular Sciences, 2020 CRISPR/Cas technologies have advanced dramatically in recent years. Many different systems with new properties have been characterized and a plethora of hybrid CRISPR/Cas systems able to modify the epigenome, regulate transcription, and correct mutations in DNA and RNA have been devised.
Dmitry Kostyushev +6 more
openaire +3 more sources
Nature Nanotechnology, 2022 Genome editing holds great potential for cancer treatment due to the ability to precisely inactivate or repair cancer-related genes. However, delivery of CRISPR/Cas to solid tumours for efficient cancer therapy remains challenging.
Di Zhang +10 more
semanticscholar +1 more source
Open Access Indonesian Journal of Medical Reviews, 2021 For thousands of years, humans have felt the need to understand the world around them—and ultimately manipulate it to best serve their needs. There are always ethical questions to address, especially when the manipulation involves the human genome. There is currently an urgent need to actively pursue those conversations as com- mercial gene sequencing ...
openaire +3 more sources
Engineered Cas9 extracellular vesicles as a novel gene editing tool
Journal of Extracellular Vesicles, 2022 Extracellular vesicles (EVs) have shown promise as biological delivery vehicles, but therapeutic applications require efficient cargo loading. Here, we developed new methods for CRISPR/Cas9 loading into EVs through reversible heterodimerization of Cas9 ...
X. Osteikoetxea +14 more
semanticscholar +1 more source
Efficient generation of brain organoids using magnetized gold nanoparticles
Scientific Reports, 2023 Brain organoids, which are three-dimensional cell culture models, have the ability to mimic certain structural and functional aspects of the human brain.
Hongwon Kim +3 more
doaj +1 more source
CRISPR-GPT: An LLM Agent for Automated Design of Gene-Editing Experiments [PDF]
bioRxivThe introduction of genome engineering technology has transformed biomedical research, making it possible to make precise changes to genetic information.
Yuanhao Qu +9 more
semanticscholar +1 more source