Results 31 to 40 of about 8,339,405 (403)

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo. [PDF]

, 2018
Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5 end can enhance the gene editing efficiency of Cpf1 both in cells
Chong, Anthony, Chu, Hunghao, Fellmann, Christof, Jiang, Fuguo, Lee, Kunwoo, Liu, Hui, Mackley, Vanessa, Murthy, Niren, Park, Hyo, Rao, Anirudh, Wu, Joann   +10 more
core   +2 more sources

Engineered Cas9 extracellular vesicles as a novel gene editing tool

Journal of Extracellular Vesicles, 2022
Extracellular vesicles (EVs) have shown promise as biological delivery vehicles, but therapeutic applications require efficient cargo loading. Here, we developed new methods for CRISPR/Cas9 loading into EVs through reversible heterodimerization of Cas9 ...
X. Osteikoetxea, A. Silva, Elisa Lázaro-Ibáñez, Nikki Salmond, O. Shatnyeva, Josia Stein, Jan Schick, Stephen Wren, J. Lindgren, M. Firth, Alexandra Madsen, L. Mayr, R. Overman, Rick Davies, N. Dekker   +14 more
semanticscholar   +1 more source

CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease

Translational Neurodegeneration, 2022
Background Aberrant DNA methylation patterns have been observed in neurodegenerative diseases, including Alzheimer's disease (AD), and dynamic changes in DNA methylation are closely associated with the onset and progression of these diseases ...
Hanseul Park, Jaein Shin, Yunkyung Kim, Takashi Saito, Takaomi C. Saido, Jongpil Kim   +5 more
doaj   +1 more source

Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41

mBio, 2022
Previous studies suggest that short peptides from the heptad repeat 2 (HR2) domain of gp41 expressed on the cell surface are more potent inhibitors of HIV-1 entry than soluble analogs.
Alexandra Maslennikova, Natalia Kruglova, Svetlana Kalinichenko, Dmitriy Komkov, Mikhail Shepelev, Dmitriy Golubev, Andrei Siniavin, Andrei Vzorov, Alexander Filatov, Dmitriy Mazurov   +9 more
doaj   +1 more source

Ethics in Gene Editing

Open Access Indonesian Journal of Medical Reviews, 2021
For thousands of years, humans have felt the need to understand the world around them—and ultimately manipulate it to best serve their needs. There are always ethical questions to address, especially when the manipulation involves the human genome. There is currently an urgent need to actively pursue those conversations as com- mercial gene sequencing ...
openaire   +2 more sources

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]

E3S Web of Conferences, 2020
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu., Kubekina Marina V., Bruter Alexandra V., Isaeva Albina G., Koshchaev Andrey G.   +4 more
doaj   +1 more source

Harnessing the Potential of Human Pluripotent Stem Cells and Gene Editing for the Treatment of Retinal Degeneration [PDF]

, 2017
PURPOSE OF REVIEW: A major cause of visual disorders is dysfunction and/or loss of the light-sensitive cells of the retina, the photoreceptors. To develop better treatments for patients, we need to understand how inherited retinal disease mutations ...
Ali, RR, Georgiadis, A, Ovando-Roche, P, Pearson, RA, Smith, AJ   +4 more
core   +1 more source

Synergistic power of genomic selection, assisted reproductive technologies, and gene editing to drive genetic improvement of cattle

CABI Agriculture and Bioscience, 2022
Genetic improvement of cattle around the globe has been, and will continue to be, an important driver of animal agriculture sustainability. There are several reproductive and molecular biotechnologies that are used in genetic improvement of cattle, and ...
M. Mueller, Alison L. Van Eenennaam
semanticscholar   +1 more source

Cell Surface-Expressed GPI-Anchored Peptides from the CHR Domain of gp41 Are Potent Inhibitors of HIV-1 Fusion

Proceedings, 2020
Current antiretroviral therapy efficiently suppresses viral replication but cannot eliminate latent HIV reservoirs. Moreover, the associated high costs, side effects, and drug resistance have stimulated a need for the development of alternative methods ...
Aleksandra Maslennikova, Dmitriy Komkov, Anastasia Zotova, Dmitriy Mazurov   +3 more
doaj   +1 more source

Application of the CRISPR/Cas9-based gene editing technique in basic research, diagnosis, and therapy of cancer

Molecular Cancer, 2021
The 2020 Nobel Prize in Chemistry was awarded to Emmanuelle Charpentier and Jennifer Doudna for the development of the Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease9 (CRISPR/Cas9) gene editing technology that ...
Huimin Zhang, Chunhong Qin, C. An, Xiwang Zheng, S. Wen, Wenjie Chen, Xianfang Liu, Zhenghua Lv, Pingchang Yang, Wei Xu, Wei Gao, Yongyan Wu   +11 more
semanticscholar   +1 more source