Results 31 to 40 of about 7,473,287 (361)

CRISPR/Cas9 gene editing: a new approach for overcoming drug resistance in cancer

Cellular & Molecular Biology Letters, 2022
The CRISPR/Cas9 system is an RNA-based adaptive immune system in bacteria and archaea. Various studies have shown that it is possible to target a wide range of human genes and treat some human diseases, including cancers, by the CRISPR/Cas9 system.
Mostafa Vaghari-Tabari, Parisa Hassanpour, Fatemeh Sadeghsoltani, Faezeh Malakoti, Forough Alemi, D. Qujeq, Z. Asemi, B. Yousefi   +7 more
semanticscholar   +1 more source

APOE ε4-dependent effects on the early amyloid pathology in induced neurons of patients with Alzheimer’s disease

Translational Neurodegeneration, 2022
Background The ε4 allele of apolipoprotein E (APOE ε4) is the strongest known genetic risk factor for late-onset Alzheimer’s disease (AD), associated with amyloid pathogenesis. However, it is not clear how APOE ε4 accelerates amyloid-beta (Aβ) deposition
Hongwon Kim, Siyoung Kim, Byounggook Cho, Jaein Shin, Jongpil Kim   +4 more
doaj   +1 more source

A beginner's guide to gene editing [PDF]

Experimental Physiology, 2018
New Findings What is the topic of this review? This review summarizes the development of gene editing from early proof‐of‐concept studies in the 1980s to contemporary programmable and RNA‐guided nucleases, which enable rapid and precise alteration of DNA sequences of almost any living cell. What advances does it highlight?
Harrison, PT, Hart, S
openaire   +5 more sources

CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia.

New England Journal of Medicine, 2020
Transfusion-dependent β-thalassemia (TDT) and sickle cell disease (SCD) are severe monogenic diseases with severe and potentially life-threatening manifestations. BCL11A is a transcription factor that represses γ-globin expression and fetal hemoglobin in
H. Frangoul, D. Altshuler, M. Cappellini, Yi-Shan Chen, J. Domm, Brenda K. Eustace, J. Foell, J. de la Fuente, S. Grupp, R. Handgretinger, Tony W. Ho, A. Kattamis, A. Kernytsky, J. Lekstrom-Himes, Amanda M Li, F. Locatelli, M. Mapara, M. de Montalembert, D. Rondelli, Akshay Sharma, S. Sheth, S. Soni, M. Steinberg, D. Wall, Angela Yen, S. Corbacioglu   +25 more
semanticscholar   +1 more source

Engineering T-Cell Resistance to HIV-1 Infection via Knock-In of Peptides from the Heptad Repeat 2 Domain of gp41

mBio, 2022
Previous studies suggest that short peptides from the heptad repeat 2 (HR2) domain of gp41 expressed on the cell surface are more potent inhibitors of HIV-1 entry than soluble analogs.
Alexandra Maslennikova, Natalia Kruglova, Svetlana Kalinichenko, Dmitriy Komkov, Mikhail Shepelev, Dmitriy Golubev, Andrei Siniavin, Andrei Vzorov, Alexander Filatov, Dmitriy Mazurov   +9 more
doaj   +1 more source

Synergistic power of genomic selection, assisted reproductive technologies, and gene editing to drive genetic improvement of cattle

CABI Agriculture and Bioscience, 2022
Genetic improvement of cattle around the globe has been, and will continue to be, an important driver of animal agriculture sustainability. There are several reproductive and molecular biotechnologies that are used in genetic improvement of cattle, and ...
M. Mueller, Alison L. Van Eenennaam
semanticscholar   +1 more source

CRISPR/dCas9-Dnmt3a-mediated targeted DNA methylation of APP rescues brain pathology in a mouse model of Alzheimer’s disease

Translational Neurodegeneration, 2022
Background Aberrant DNA methylation patterns have been observed in neurodegenerative diseases, including Alzheimer's disease (AD), and dynamic changes in DNA methylation are closely associated with the onset and progression of these diseases ...
Hanseul Park, Jaein Shin, Yunkyung Kim, Takashi Saito, Takaomi C. Saido, Jongpil Kim   +5 more
doaj   +1 more source

High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA

Journal of Experimental Medicine, 2022
Effective and precise gene editing of T lymphocytes is critical for advancing our understanding of T cell biology and the development of next-generation cellular therapies.
Soyoung A Oh, K. Senger, Shravan Madireddi, Ilseyar Akhmetzyanova, Isabel E Ishizuka, S. Tarighat, Jerry H Lo, David Graeme Shaw, B. Haley, S. Rutz   +9 more
semanticscholar   +1 more source

Cell Surface-Expressed GPI-Anchored Peptides from the CHR Domain of gp41 Are Potent Inhibitors of HIV-1 Fusion

Proceedings, 2020
Current antiretroviral therapy efficiently suppresses viral replication but cannot eliminate latent HIV reservoirs. Moreover, the associated high costs, side effects, and drug resistance have stimulated a need for the development of alternative methods ...
Aleksandra Maslennikova, Dmitriy Komkov, Anastasia Zotova, Dmitriy Mazurov   +3 more
doaj   +1 more source

Gene editing CRISPR/Cas9 system for producing cows with hypoallergenic milk on the background of a beta-lactoglobulin gene knockout [PDF]

E3S Web of Conferences, 2020
Beta-lactoglobulin is the main allergen of cow’s milk. Modern approaches to reducing the allergenicity of milk require significant costs for its fermentation. An alternative approach could be the creation of productive breeds with a knockout for the gene
Silaeva Yulia Yu., Kubekina Marina V., Bruter Alexandra V., Isaeva Albina G., Koshchaev Andrey G.   +4 more
doaj   +1 more source