Bacteria herald a new era of gene editing. [PDF]
, 2013 The demonstration that nucleases guided by bacterial RNA can disrupt human genes represents a landmark in the rapidly developing field of genome ...
Segal, David J
core
Promoter keyholes enable specific and persistent multi-gene expression programs in primary T cells without genome modification [PDF]
, 2020 Non-invasive epigenome editing is a promising strategy for engineering gene expression programs, yet potency, specificity, and persistence remain challenging.
Acosta, Reyes +25 more
core
Imaging cell lineage with a synthetic digital recording system [PDF]
, 2020 Cell lineage plays a pivotal role in cell fate determination. Chow et al. demonstrate the use of an integrase-based synthetic barcode system called intMEMOIR, which uses the serine integrase Bxb1 to perform irreversible nucleotide edits.
Budde, Mark W. +11 more
core +1 more source
Genome Editing with Crispr-Cas9 Systems: Basic Research and Clinical Applications [PDF]
, 2017 BACKGROUND: Recently established genome editing technologies will open new avenues for biological research and development. Human genome editing is a powerful tool which offers great scientific and therapeutic potential.CONTENT: Genome editing using the ...
Dewi, N. M. (Nurrani) +2 more
core +4 more sources
Generation of RAG2 Knockout Immune-Deficient Miniature Pigs
AnimalsRecombination-activating genes (RAGs) play a crucial role in the V(D)J recombination process and the development of immune cells. The development of the immune system and its mechanisms in pigs exhibit greater similarity to those of humans compared to ...
Jing Wang +8 more
doaj +1 more source
Heat increases the editing efficiency of human papillomavirus E2 gene by inducing upregulation of APOBEC3A and 3G [PDF]
, 2017 Apolipoprotein B mRNA-editing catalytic polypeptide (APOBEC) 3 proteins have been identified as potent viral DNA mutators and have broad antiviral activity.
Chen, Hongduo +9 more
core +1 more source
Artificial and Natural Genetic Information Processing [PDF]
, 2017 Conventional methods of genetic engineering and more recent genome editing techniques focus on identifying genetic target sequences for manipulation.
Witzany, Guenther
core
Methods for Scarless, Selection-Free Generation of Human Cells and Allele-Specific Functional Analysis of Disease-Associated SNPs and Variants of Uncertain Significance. [PDF]
, 2017 With the continued emergence of risk loci from Genome-Wide Association studies and variants of uncertain significance identified from patient sequencing, better methods are required to translate these human genetic findings into improvements in public ...
Carvajal-Carmona, Luis G +4 more
core +4 more sources
Barrier-to-autointegration factor 1 protects against a basal cGAS-STING response [PDF]
, 2020 Although the pathogen recognition receptor pathways that activate cell-intrinsic antiviral responses are well delineated, less is known about how the host regulates this response to prevent sustained signaling and possible immune-mediated damage. Using a
Artyomov, Maxim +11 more
core +2 more sources
Switchable genome editing via genetic code expansion [PDF]
, 2018 Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light.
Asami, Maki +5 more
core +2 more sources