Results 51 to 60 of about 8,339,405 (403)

CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. [PDF]

, 2020
With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Chen, Qu, Fu, Xuemei, Kim, Jinchul, Liu, Jingfeng, Tang, Qingshuang, Xu, Shuxiang, Xu, Yang   +6 more
core  

NG peptides: A novel family of neurophysin-associated neuropeptides [PDF]

, 2010
NOTICE: this is the author’s version of a work that was accepted for publication in GENE. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be ...
Bromham, De Bree, De Bree, Elphick, Gwee, Inoue, Ivell, Iwakoshi, Kato, Kawada, Legros, Maurice R. Elphick, Putnam, Reinscheid, Roth, Stafflinger, Terhzaz, Ukena, Van Kesteren, Verleyen, Xu   +20 more
core   +1 more source

Gene editing and gene regulation with CRISPR [PDF]

Experimental Physiology, 2018
Genome editing enables precise changes to be made in the genome of living cells. The technique was originally developed in the 1980’s but largely limited to use in mice. The discovery that a targeted double stranded break (DSB) at a unique site in the genome, close to the site to be changed, could substantially increase the efficiency of editing ...
Patrick Harrison, Stephen Hart
openaire   +3 more sources

CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia.

New England Journal of Medicine, 2020
Transfusion-dependent β-thalassemia (TDT) and sickle cell disease (SCD) are severe monogenic diseases with severe and potentially life-threatening manifestations. BCL11A is a transcription factor that represses γ-globin expression and fetal hemoglobin in
H. Frangoul, D. Altshuler, M. Cappellini, Yi-Shan Chen, J. Domm, Brenda K. Eustace, J. Foell, J. de la Fuente, S. Grupp, R. Handgretinger, Tony W. Ho, A. Kattamis, A. Kernytsky, J. Lekstrom-Himes, Amanda M Li, F. Locatelli, M. Mapara, M. de Montalembert, D. Rondelli, Akshay Sharma, S. Sheth, S. Soni, M. Steinberg, D. Wall, Angela Yen, S. Corbacioglu   +25 more
semanticscholar   +1 more source

Applications and challenges of CRISPR-Cas gene-editing to disease treatment in clinics

Precision Clinical Medicine, 2021
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated systems (Cas) are efficient tools for targeting specific genes for laboratory research, agricultural engineering, biotechnology, and human disease treatment.
Wenyi Liu, Luoxi Li, Jianxin Jiang, Min Wu, Ping Lin   +4 more
semanticscholar   +1 more source

Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure

Nature Communications, 2022
Prime editors can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here the authors introduce same-sense mutations into pegRNAs to increase base-editing efficiency and the ...
Xiaosa Li, Lina Zhou, Bao-Qing Gao, Guangye Li, Xiao Wang, Ying Wang, Jia Wei, Wenyan Han, Zixian Wang, Jifang Li, Runze Gao, Junjie Zhu, Wenchao Xu, Jing Wu, Bei Yang, Xiaodong Sun, Li Yang, Jia Chen   +17 more
doaj   +1 more source

HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread

Viruses, 2022
So far, only two retroviruses, human immunodeficiency virus (HIV) (type 1 and 2) and human T-cell lymphotropic virus type 1 (HTLV-1), have been recognized as pathogenic for humans. Both viruses mainly infect CD4+ T lymphocytes.
Svetlana Kalinichenko, Dmitriy Komkov, Dmitriy Mazurov   +2 more
doaj   +1 more source

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]

, 2019
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan, Kwon, Jin, Meyer, Vera, Schütze, Tabea, Spohner, Sebastian   +4 more
core   +1 more source

Functional characterization of tumor-specific CRISPR-directed gene editing as a combinatorial therapy for the treatment of solid tumors

Molecular Therapy: Oncology
As we pursue clinical applications for CRISPR-directed gene editing in overcoming resistance to anticancer drugs, we have focused on genetic disruption of the transcription factor, NRF2, a master regulator of cellular stress and detoxification. The level
Kelly H. Banas, Pawel A. Bialk, Natalia Rivera-Torres, Katelynn Owens, Tori N. Reiner, Kristen M. Pisarcik, Nicole Haas, Emily Gielda, Komal Khan, Krishna Priya Narra, Eric B. Kmiec   +10 more
doaj   +1 more source

Gene editing

Postępy Biochemii, 2018
Development of the gene engineering techniques has raised worries that they will be used for construction of organism endangering humansand environment. In 1975 at the Asilomar conference, geneticists from many countries decided that genetic engineering brings more benefitsthan threats. In last years a new CRISPR-Cas technique emerged .
openaire   +3 more sources