Results 41 to 50 of about 7,937 (203)

Overcoming the Specific Toxicity of Large Plasmids Electrotransfer in Primary Cells In Vitro

Molecular Therapy: Nucleic Acids, 2016
Gene electrotransfer is a safe and efficient nonviral technique for the transfer of nucleic acids of all sizes. Using a small reporter plasmid (3.5 kbp), electrotransfer of more than 90% of the cells, with ≃70% viability, can be routinely achieved even ...
Léa L Lesueur, Lluis M Mir, Franck M André   +2 more
doaj   +1 more source

Combined effect of heat and corona charge on molecular delivery to a T cell line in vitro.

PLoS ONE, 2023
With the rapid increase of gene and immunotherapies for treating cancer, there is a need to efficiently transfect cells. Previous studies suggest that electrotransfer can provide a non-viral method for gene delivery.
Molly A Skinner, Alex Otten, Andrew Hoff, Mark Jaroszeski   +3 more
doaj   +1 more source

Therapeutic potential of bleomycin plus suicide or interferon-β gene transfer combination for spontaneous feline and canine melanoma [PDF]

, 2017
We originated and characterized melanoma cell lines derived from tumors of two feline and two canine veterinary patients. These lines reestablished the morphology, physiology and cell heterogeneity of their respective parental tumors.
Agnetti, Lucrecia, Finocchiaro, Liliana Maria Elena, Fondello, Chiara, Glikin, Gerardo Claudio, Villaverde, Marcela Solange   +4 more
core   +1 more source

Muscle-specific overexpression of AdipoR1 or AdipoR2 gives rise to common and discrete local effects whilst AdipoR2 promotes additional systemic effects [PDF]

, 2017
Hypoadiponectinemia and adiponectin resistance are implicated in the aetiology of obesity-related cardiometabolic disorders, hence represent a potential therapeutic axis.
A Rodriguez, AM Delaigle, CR Bruce, DC Henstridge, DJ Dyck, F Almabouada, GS Hotamisligil, H Kanda, H Tanabe, H Xu, HK Charlton, IJ Hickman, IP Chou, J Jortay, JL Parker-Duffen, JT Heiker, K Dadson, K Tomita, KL Mullen, KL Mullen, KL Mullen, L Tian, M Bjursell, M Iwabu, MB Chen, MH Lee, N Luo, P Zhang, Q Ding, S Keshvari, S Keshvari, SA Patel, T Sente, T Yamauchi, T Yamauchi, T Yamauchi, T Yamauchi, WL Holland, Y Liu, Y Liu, Y Ma, Y Wang, ZV Wang   +42 more
core   +3 more sources

Monopolar gene electrotransfer enhances plasmid DNA delivery to skin

Bioelectrochemistry, 2021
A novel monopolar electroporation system and methodologies were developed for in vivo electroporation intended for potential clinical applications such as gene therapy. We hypothesized that an asymmetric anode/cathode electrode applicator geometry could produce favorable electric fields for electroporation, without the typical drawback associated with ...
Anna Bulysheva, Loree Heller, Michael Francis, Frency Varghese, Carly Boye, Richard Heller   +5 more
openaire   +3 more sources

West Nile premembrane-envelope genetic vaccine encoded as a chimera containing the transmembrane and cytoplasmic domains of a lysosome-associated membrane protein: increased cellular concentration of the transgene product, targeting to the MHC II compartment, and enhanced neutralizing antibody response [PDF]

, 2004
A genetic vaccine for West Nile virus (WN) has been synthesized with the WN premembrane-envelope (WN preM-E) gene sequences encoded as a chimera with the transmembrane and carboxyl terminal domains of the lysosome-associated membrane protein (LAMP).
Anwar, Azlinda, Chandrasekaran, Ananth, Ng, Mah Lee, Marques, Ernesto, August, J. Thomas   +4 more
core   +1 more source

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

Biological Procedures Online, 2009
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain
Eriksen Jens, Gehl Julie, Hojman Pernille   +2 more
doaj   +3 more sources

Immune cell populations in the tumour environment following calcium electropora­tion for cutaneous metastasis: a histopathological study

Acta Oncologica
Background and Purpose: Calcium electroporation (CaEP) involves injecting calcium into tumour tissues and using electrical pulses to create membrane pores that induce cell death.
Mille Vissing, Sandra Sinius Pouplier, Lars Munch Larsen, Stine Krog Frandsen, Alexey Lodin, Anne-Vibeke Lænkholm, Julie Gehl   +6 more
doaj   +1 more source

Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro

BioMedical Engineering OnLine, 2007
Background Gene electrotransfer is a non-viral method used to transfer genes into living cells by means of high-voltage electric pulses. An exposure of a cell to an adequate amplitude and duration of electric pulses leads to a temporary increase of cell ...
Rols Marie-Pierre, Teissié Justin, Čorović Selma, Kandušer Maša, Faurie Cécile, Reberšek Matej, Miklavčič Damijan   +6 more
doaj   +1 more source

Gene therapy by electroporation for the treatment of chronic renal failure in companion animals [PDF]

, 2009
Background Growth hormone-releasing hormone (GHRH) plasmid-based therapy for the treatment of chronic renal failure and its complications was examined. Companion dogs (13.1 ± 0.8 years, 29.4 ± 5.01 kg) and cats (13.2 ± 0.9 years, 8.5 ± 0.37 kg) received ...
Patricia A Brown, Angela M Bodles-Brakhop, Melissa A Pope, Ruxandra Draghia-Akli   +3 more
core   +2 more sources