Results 41 to 50 of about 6,790,032 (188)

A microfluidic biochip for the nanoporation of living cells [PDF]

, 2011
International audienceThis paper deals with the development of a microfluidic biochip for the exposure of living cells to nanosecond pulsed electric fields (nsPEF). When exposed to ultra short electric pulses (typical duration of 3-10ns), disturbances on
Arnaud-Cormos, Delia, Dalmay, Claire, Français, Olivier, Joubert, Vanessa, Le Pioufle, Bruno, Lévêque, Philippe, Mir, Lluis M, Silve, Aude, Villemejane, Julien   +8 more
core   +2 more sources

Electropermeabilization of inner and outer cell membranes with microsecond pulsed electric field. Quantitative study with calcium ions [PDF]

, 2017
Microsecond pulsed electric fields (mu sPEF) permeabilize the plasma membrane (PM) and are widely used in research, medicine and biotechnology. For internal membranes permeabilization, nanosecond pulsed electric fields (nsPEF) are applied but this ...
Andrã©, Franck M., Denzi, Agnese, Hanna, Hanna, Liberti, Micaela, Mir, Lluis M.   +4 more
core   +2 more sources

Numerical optimization of gene electrotransfer into muscle tissue [PDF]

BioMedical Engineering OnLine, 2010
Abstract Background Electroporation-based gene therapy and DNA vaccination are promising medical applications that depend on transfer of pDNA into target tissues with use of electric pulses. Gene electrotransfer efficiency depends on electrode configuration and electric pulse parameters, which determine the electric ...
Miklavcic Damijan, Corovic Selma, Zupanic Anze, Pavlin Mojca   +3 more
openaire   +3 more sources

Differentiation of ruminant transmissible spongiform encephalopathy isolate types, including bovine spongiform encephalopathy and CH1641 scrapie [PDF]

, 2010
With increased awareness of the diversity of transmissible spongiform encephalopathy (TSE) strains in the ruminant population, comes an appreciation of the need for improved methods of differential diagnosis. Exposure to bovine spongiform encephalopathy (
Bossers, A., Jacobs, J.G., Keulen, L.J.M., van, Langeveld, J.P.M., Sauer, M., Tang, Y.   +5 more
core   +2 more sources

Gene electrotransfer enhanced by nanosecond pulsed electric fields. [PDF]

Mol Ther Methods Clin Dev, 2014
The impact of nanosecond pulsed electric fields (nsPEFs) on gene electrotransfer has not been clearly demonstrated in previous studies. This study was conducted to evaluate the influence of nsPEFs on the delivery of plasmids encoding luciferase or green fluorescent protein and subsequent expression in HACAT keratinocyte cells.
Guo S, Jackson DL, Burcus NI, Chen YJ, Xiao S, Heller R.   +5 more
europepmc   +5 more sources

Muscle-specific overexpression of AdipoR1 or AdipoR2 gives rise to common and discrete local effects whilst AdipoR2 promotes additional systemic effects [PDF]

, 2017
Hypoadiponectinemia and adiponectin resistance are implicated in the aetiology of obesity-related cardiometabolic disorders, hence represent a potential therapeutic axis.
A Rodriguez, AM Delaigle, CR Bruce, DC Henstridge, DJ Dyck, F Almabouada, GS Hotamisligil, H Kanda, H Tanabe, H Xu, HK Charlton, IJ Hickman, IP Chou, J Jortay, JL Parker-Duffen, JT Heiker, K Dadson, K Tomita, KL Mullen, KL Mullen, KL Mullen, L Tian, M Bjursell, M Iwabu, MB Chen, MH Lee, N Luo, P Zhang, Q Ding, S Keshvari, S Keshvari, SA Patel, T Sente, T Yamauchi, T Yamauchi, T Yamauchi, T Yamauchi, WL Holland, Y Liu, Y Liu, Y Ma, Y Wang, ZV Wang   +42 more
core   +3 more sources

West Nile premembrane-envelope genetic vaccine encoded as a chimera containing the transmembrane and cytoplasmic domains of a lysosome-associated membrane protein: increased cellular concentration of the transgene product, targeting to the MHC II compartment, and enhanced neutralizing antibody response [PDF]

, 2004
A genetic vaccine for West Nile virus (WN) has been synthesized with the WN premembrane-envelope (WN preM-E) gene sequences encoded as a chimera with the transmembrane and carboxyl terminal domains of the lysosome-associated membrane protein (LAMP).
Anwar, Azlinda, Chandrasekaran, Ananth, Ng, Mah Lee, Marques, Ernesto, August, J. Thomas   +4 more
core   +1 more source

Therapeutic potential of bleomycin plus suicide or interferon-β gene transfer combination for spontaneous feline and canine melanoma [PDF]

, 2017
We originated and characterized melanoma cell lines derived from tumors of two feline and two canine veterinary patients. These lines reestablished the morphology, physiology and cell heterogeneity of their respective parental tumors.
Agnetti, Lucrecia, Finocchiaro, Liliana Maria Elena, Fondello, Chiara, Glikin, Gerardo Claudio, Villaverde, Marcela Solange   +4 more
core   +1 more source

Mechanisms to Evade the Phagocyte Respiratory Burst Arose by Convergent Evolution in Typhoidal Salmonella Serovars. [PDF]

, 2018
Typhoid fever caused by Salmonella enterica serovar (S.) Typhi differs in its clinical presentation from gastroenteritis caused by S. Typhimurium and other non-typhoidal Salmonella serovars.
Bäumler, Andreas J, Cobb, Brian A, Hiyoshi, Hirotaka, Lock, Gabriel, Raffatellu, Manuela, Saechao, Cheng, Wangdi, Tamding   +6 more
core   +2 more sources

Recellularized human dermis for testing gene electrotransfer ex vivo. [PDF]

Biomed Mater, 2016
Gene electrotransfer (GET) is a proven and valuable tool for in vivo gene delivery to a variety of tissues such as skin, cardiac muscle, skeletal muscle, and tumors, with controllable gene delivery and expression levels. Optimizing gene expression is a challenging hurdle in preclinical studies, particularly for skin indications, due to differences in ...
Bulysheva AA, Burcus N, Lundberg C, Edelblute CM, Francis MP, Heller R.   +5 more
europepmc   +4 more sources