Results 31 to 40 of about 3,123,960 (258)

Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination

, 2021
Genetic engineering of industrial cell lines often requires knockout of multiple endogenous genes. Tools like CRISPR-Cas9 have enabled serial or parallelized gene disruption in a wide range of industrial organisms, but common practices for the screening ...
Neil C. Dalvie (3287133), Timothy Lorgeree, Timothy Lorgeree (11820308), Andrew M. Biedermann, Kerry R. Love (4490911), Neil C. Dalvie, Andrew M. Biedermann (10735208), Kerry R. Love, J. Christopher Love, J. Christopher Love (159667)   +9 more
core   +1 more source

Generation of Fibroblasts Lacking the Sal-like 1 Gene by Using Transcription Activator-like Effector Nuclease-mediated Homologous Recombination [PDF]

Asian-Australasian Journal of Animal Sciences, 2016
The Sal-like 1 gene (Sall1) is essential for kidney development, and mutations in this gene result in abnormalities in the kidneys. Mice lacking Sall1 show agenesis or severe dysgenesis of the kidneys.
Se Eun Kim, Ji Woo Kim, Yeong Ji Kim, Deug-Nam Kwon, Jin-Hoi Kim, Man-Jong Kang   +5 more
doaj   +1 more source

Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats

陆军军医大学学报, 2023
Objective To observe the sleep/wake behaviors and EEG power spectrum in melatonin type 3 receptor (MT3R/Nqo2) knockout rats with CRISPR/Cas9 gene editing tool in order to explore the receptor mechanism of melatonin in regulation of sleep/wake behaviors ...
LI Xin, LI Shiyin, LUO Fenlan, REN Shuancheng, REN Shuancheng   +4 more
doaj   +1 more source

The LmSNF1 gene is required for pathogenicity in the canola blackleg pathogen Leptosphaeria maculans. [PDF]

PLoS ONE, 2014
Leptosphaeria maculans is a fungal pathogen causing blackleg in canola. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall degrading enzymes (CWDEs).
Jie Feng, Hui Zhang, Stephen E Strelkov, Sheau-Fang Hwang   +3 more
doaj   +1 more source

Loss of the actin remodeler Eps8 causes intestinal defects and improved metabolic status in mice. [PDF]

, 2010
In a variety of organisms, including mammals, caloric restriction improves metabolic status and lowers the incidence of chronic-degenerative diseases, ultimately leading to increased lifespan.
Soppo, Charlotte Blanche Ekalle, Martin Klingenspor, Eugenio Scanziani, Hrabě de Angelis, M., Fabrizio Bianchi, Di Fiore, Pier Paolo, B. Pozzi, Offenhäuser Nina, Martin Hrabé de Angelis, Klingenspor Martin, B. Rathkolb, Horsch Marion, Horsch, Marion, Rathkolb, B., Nicole Ehrhardt, Wolf Eckhard, V. Gailus Durner, Tacchetti Carlo, Beckers, Johannes, Jan Rozman, Gailus-Durner, Valérie, Ralf Elvert, Hrabé de Angelis, Martin, Wolf, E., Ehrhardt, Nicole, Johannes Beckers, Elvert, Ralf, Tocchetti, Arianna, Pier Paolo Di Fiore, F. Bianchi, Elvert, R., A. Tocchetti, Horsch, M., Klingenspor, Martin, Bianchi, F., Moerth Corinna, Carlo Tacchetti, Ehrhardt Nicole, Pozzi, Benedetta, Scanziani Eugenio, Gagliani, M.C., Offenhäuser, N., Bianchi Fabrizio, Giorgio Scita, Birgit Rathkolb, M. C. Gagliani, Ehrhardt, N., Scita, G., Fuchs, Helmut, di Fiore, P.P., J. Beckers, Zani Fabio, Corinna Moerth, Eckhard Wolf, de Angelis Martin Hrabé, Mörth, C., Pozzi, B., Fuchs Helmut, M. Klingenspor, Beckers Johannes, M. Horsch, Tacchetti, C., Nina Offenhäuser, Rathkolb, Birgit, G. Scita, Tacchetti, Carlo, Bianchi, Fabrizio, Rozman, J., Rozman, Jan, Maria Cristina Gagliani, Charlotte Blanche Ekalle Soppo, Arianna Tocchetti, Ekalle Soppo Charlotte Blanche, Zani, F., N. Ehrhardt, Fuchs, H., J. Rozman, P.P. Di Fiore, Soppo, C.B.E., Valérie Gailus-Durner, Gailus-Durner, V., Gagliani, Maria Cristina, Moerth, Corinna, E. Scanziani, Scanziani, Eugenio, Helmut Fuchs, N. Offenhäuser, C. Tacchetti, Offenhäuser, Nina, Scita, Giorgio, Fabio Zani, C. Moerth, E. Wolf, Tocchetti, A., Elvert Ralf, Di Fiore Pier Paolo, M. Hrabé de Angelis, Tocchetti Arianna, Beckers, J., F. Zani, C. B. Soppo, Gagliani Maria Cristina, Scita Giorgio, Zani, Fabio, Scanziani, E., Klingenspor, M., Rathkolb Birgit, R. Elvert, Marion Horsch, Benedetta Pozzi, Pozzi Benedetta, Gailus-Durner Valérie, Wolf, Eckhard, Rozman Jan, H. Fuchs   +114 more
core   +2 more sources

Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology

G3: Genes, Genomes, Genetics, 2016
The present study established an efficient genome editing approach for the construction of stable transgenic cell lines of the domestic chicken (Gallus gallus domesticus). Our objectives were to facilitate the breeding of high-yield, high-quality chicken
Qisheng Zuo, Yinjie Wang, Shaoze Cheng, Chao Lian, Beibei Tang, Fei Wang, Zhenyu Lu, Yanqing Ji, Ruifeng Zhao, Wenhui Zhang, Kai Jin, Jiuzhou Song, Yani Zhang, Bichun Li   +13 more
doaj   +1 more source

CRISPR/Cas9 Targeted Mutagenesis for Functional Genetics in Maize

Plants, 2021
The CRISPR/Cas9-based system for targeted mutagenesis has become an indispensable tool for functional genetics in plants. CRISPR/Cas9 allows users to generate loss-of-function alleles in genes of interest with precision and in a simple-to-use system ...
Charles T. Hunter
doaj   +1 more source

Association of the CCR5 gene with juvenile idiopathic arthritis [PDF]

, 2010
The CC chemokine receptor 5 (CCR5) has been shown to be important in the recruitment of T-helper cells to the synovium, where they accumulate, drive the inflammatory process and the consequent synovitis and joint destruction.
Martin, P., BSPAR Study Group, Barton, A., UKRAG Consortium, Worthington, J., Gardner-Medwin, J. [CAPS and BSPAR Study Groups], Thomson, W., W Thomson, Flynn, E., A Hinks, E Flynn, J Packham, Packham, J., Hinks, A., S Eyre, J Worthington, Eyre, S.; id_orcid, Eyre, S., Barton, A.; id_orcid, P Martin, Childhood Arthritis Prospective Study (CAPS), Martin, P.; id_orcid, A Barton   +22 more
core   +1 more source

sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing

Cell Reports, 2019
Summary: Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene editing. High CRISPR/Cas9-mediated gene knockout efficiencies are essential for genetic screens and critically depend on the properties of the sgRNAs used.
Robin Graf, Xun Li, Van Trung Chu, Klaus Rajewsky   +3 more
doaj   +1 more source

Update on Viral Gene Therapy Clinical Trials for Retinal Diseases [PDF]

, 2022
In 2001, the first large animal was successfully treated with a gene therapy that restored its vision. Lancelot, the Briard dog that was treated, suffered from a human childhood blindness called Leber's congenital amaurosis type 2.
Cheng, Shun-Yun, Punzo, Claudio
core   +1 more source