Results 11 to 20 of about 3,123,960 (258)

PEX3 gene knockout influences recombinant xylanase expression by Komagataella phaffii

Synthetic and Systems Biotechnology
Komagataella phaffii is a methylotrophic yeast harboring a tightly regulated alcohol oxidase promoter (PAOX1), which is now widely used for recombinant protein production.
Ziwei Zhou, Wenjie Cong, Mingxuan Wang, Hualan Zhou, Jianguo Zhang   +4 more
doaj   +2 more sources

Characterisation of iunH gene knockout strain from Mycobacterium tuberculosis

Memorias do Instituto Oswaldo Cruz
BACKGROUND Tuberculosis (TB) is an infectious disease caused mainly by the bacillus Mycobacterium tuberculosis. The better understanding of important metabolic pathways from M.
Anne Drumond Villela, Valnês da Silva Rodrigues Junior, Antônio Frederico Michel Pinto, Priscila Lamb Wink, Zilpa Adriana Sánchez-Quitian, Guilherme Oliveira Petersen, Maria Martha Campos, Luiz Augusto Basso, Diógenes Santiago Santos   +8 more
doaj   +2 more sources

Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases

Frontiers in Genetics, 2020
Ribosomal RNAs in all organisms are methylated. The functional role of the majority of modified nucleotides is unknown. We systematically questioned the influence of rRNA methylation in Escherichia coli on a number of characteristics of bacterial cells ...
Philipp Pletnev, Philipp Pletnev, Philipp Pletnev, Ekaterina Guseva, Anna Zanina, Sergey Evfratov, Margarita Dzama, Vsevolod Treshin, Alexandra Pogorel’skaya, Ilya Osterman, Ilya Osterman, Anna Golovina, Maria Rubtsova, Maria Rubtsova, Marina Serebryakova, Marina Serebryakova, Olga V. Pobeguts, Vadim M. Govorun, Alexey A. Bogdanov, Alexey A. Bogdanov, Olga A. Dontsova, Olga A. Dontsova, Olga A. Dontsova, Olga A. Dontsova, Petr V. Sergiev, Petr V. Sergiev, Petr V. Sergiev, Petr V. Sergiev   +27 more
doaj   +1 more source

Exploring gene knockout strategies to identify potential drug targets using genome-scale metabolic models

Scientific Reports, 2021
Research on new cancer drugs is performed either through gene knockout studies or phenotypic screening of drugs in cancer cell-lines. Both of these approaches are costly and time-consuming.
Abhijit Paul, Rajat Anand, Sonali Porey Karmakar, Surender Rawat, Nandadulal Bairagi, Samrat Chatterjee   +5 more
doaj   +1 more source

New insights of growth hormone (GH) actions from tissue-specific GH receptor knockouts in mice

Archives of Endocrinology and Metabolism, 2020
In order to provide new insights into the various activities of GH in specific tissues, recent advances have allowed for the generation of tissue-specific GHR knockout mice.
Edward O. List, Darlene E. Berryman, Elizabeth A. Jensen, Prateek Kulkarni, Savannah McKenna, John J. Kopchick   +5 more
doaj   +1 more source

"Plasmodium falciparum" transfection technology for the analysis of "var" gene regulation and knockout investigation [PDF]

, 2007
Malaria is an infectious disease caused by protozoans of the genus Plasmodium, which are injected by the bite of an infected female Anopheles mosquito during a blood meal. Out of the four species that infect humans, P. falciparum is the most important.
Bopp, Selina Elisabeth Ruth
core   +1 more source

Establishment and identification of a mouse model of Fscb/Cabyr double gene knockout

Di-san junyi daxue xuebao, 2020
Objective To construct a mouse model of targeted knockout of Fscb/Cabyr dual genes by using the CRISPR/Cas9 system, in order to found a basis for the study of the role of FSCB and CABYR protein complexe in sperm flagellum movement and capacitation ...
LI Zhongtai, LI Zhongtai, LI Yanfeng, LIU Jinyi   +3 more
doaj   +1 more source

Knocking out FLO8 gene of Candida glabrata and its effect on EPA family [PDF]

Zhenduanxue lilun yu shijian
Objective To construct a FLO8 gene knockout strain of Candida glabrata and analyze the effect of FLO8 knockout on the expression of EPA family in Candida glabrata.
ZHAO Juntao, YUAN Jie, LIU Jinyan, CHEN Kezhi, XIANG Mingjie
doaj   +1 more source

A modified TALEN-based strategy for rapidly and efficiently generating knockout mice for kidney development studies. [PDF]

PLoS ONE, 2014
The transcription activator-like effector nucleases (TALENs) strategy has been widely used to delete and mutate genes in vitro. This strategy has begun to be used for in vivo systemic gene manipulation, but not in an organ-specific manner. In this study,
Yunhong Liu, Xiaoyan Lv, Ruizhi Tan, Tianming Liu, Tielin Chen, Mi Li, Yuhang Liu, Fang Nie, Xiaoyan Wang, Puhui Zhou, Mianzhi Chen, Qin Zhou   +11 more
doaj   +1 more source

The International Gene Trap Consortium website : a portal to all publicly available gene trap cell lines in mouse [PDF]

, 2006
Gene trapping is a method of generating murine embryonic stem (ES) cell lines containing insertional mutations in known and novel genes. A number of international groups have used this approach to create sizeable public cell line repositories available ...
Chang, Patricia J., Conklin, B.R., Rossant, J., Skarnes, William C., Harper, C.A., Harper, C., Skarnes, W.C., Babbitt, Patricia C, Johns, Susan J., Chang, P., Chang, P.J., Huang, C., Yamamura, Ken-ichi, von Melchner, Harald, Hicks, Geoffrey G, Wurst, Wolfgang, Ferrin, Thomas E., Soriano, Philippe, Nord, Alex S., Ferrin, T., Morris, J.H., Hicks, G., Wurst, W., Cox, Antony V, Cox, A.V., Chang, Patricia J, Babbitt, Patricia C., Liu, S., Young, S.G., Babbitt, P.C., Meng, Elaine C, Young, Stephen G, Ruiz, Patricia, Stanford, W.L., Cox, A., Nord, A.S., Harper, Courtney A., Babbitt, P., Stanford, William L., Young, S., Huang, C.C., Huang, Conrad C, Johns, Susan J, Skarnes, W., Johns, S., Kawamoto, Michiko, Kawamoto, M., Stanford, W., Meng, E.C., von, Melchner, H., Skarnes, William C, Soriano, P., Johns, S.J., Huang, Conrad C., Morris, John H, Ferrin, T.E., Meng, Elaine C., Nord, A., Young, Stephen G., Conklin, B., Yamamura, K., Hicks, G.G., Stanford, William L, Ferrin, Thomas E, Stryke, Doug, Morris, J., von Melchner, H., Cox, Antony V., Stryke, D., Conklin, Bruce R, Harper, Courtney A, Meng, E., Morris, John H., Conklin, Bruce R., Liu, Songyan, Ruiz, P., Hicks, Geoffrey G., Nord, Alex S, Melchner, Harald von (Prof. Dr.), Rossant, Janet   +79 more
core   +1 more source