Results 1 to 10 of about 3,123,960 (258)
PLoS Genetics, 2006 RNA interference (RNAi) has been used increasingly for reverse genetics in invertebrates and mammalian cells, and has the potential to become an alternative to gene knockout technology in mammals.
Xu-Gang Xia +4 more
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Tripleknock: predicting lethal effect of three-gene knockout in bacteria by deep learning [PDF]
Scientific ReportsInvestigating the lethal effect of multi-gene knockout is essential for discovering novel antibiotic targets and metabolic engineering. Unlike single genes or gene pairs, three-gene combinations involve more intricate interactions, making experimental ...
Peter X. Geng +4 more
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Virulence, 2020 To investigate the virulence of capsular polysaccharide export protein (Wza) in carbapenem-resistant Acinetobacter baumannii and its effect on capsule formation.
Tianshui Niu +7 more
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Effective IHH gene knockout by CRISPR/Cas9 system in chicken DF-1 cells [PDF]
Poultry ScienceIndian hedgehog (IHH) gene codes an important signal molecule mediating chondrogenesis and bone development in chickens, which are key factors that affect body weight and several other significant economic traits.
Sihua Jin +8 more
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CRISPR-Cas9-induced gene knockout in zebrafish
STAR Protocols, 2022 Summary: The application of CRISPR has greatly facilitated genotype-phenotype studies of human disease models. In this protocol, we describe CRISPR-Cas9-induced gene knockout in zebrafish, utilizing purified Cas9 protein and in vitro-transcribed sgRNA ...
Raghavender Medishetti +7 more
doaj +1 more source
Simple gene knockout by single gene-directed multiplex CRISPR-Cpf1 [PDF]
Genes and DiseasesYeon-Ju Jeong +3 more
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Knockout of PKC θ gene attenuates oleic acid-induced acute lung injury via reduction of inflammation and oxidative stress [PDF]
Iranian Journal of Basic Medical Sciences, 2021 Objective(s): Acute respiratory distress syndrome resulting from acute lung injury has become a momentous clinical concern because of high morbidity and mortality in discharged patients with pulmonary and nonpulmonary diseases.
Wei Li +4 more
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Review of knockout technology approaches in bacterial drug resistance research [PDF]
PeerJ, 2023 Gene knockout is a widely used method in biology for investigating gene function. Several technologies are available for gene knockout, including zinc-finger nuclease technology (ZFN), suicide plasmid vector systems, transcription activator-like effector
Chunyu Tong +6 more
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RNAi and CRISPR/Cas9 as Functional Genomics Tools in the Neotropical Stink Bug, Euschistus heros
Insects, 2020 The Neotropical brown stink bug, Euschistus heros, is one of the most important stink bug pests in leguminous plants in South America. RNAi and CRISPR/Cas9 are important and useful tools in functional genomics, as well as in the future development of new
Deise Cagliari +3 more
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Cell Death Discovery, 2022 Corneal allograft rejection can be seen in some patients after corneal transplantation. The present study intends to investigate whether JAK2 gene knockout affects corneal allograft rejection through regulation of dendritic cells (DCs)-induced T cell ...
Jun Hu +3 more
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