Results 91 to 100 of about 1,268,532 (356)

Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates. [PDF]

, 2005
In an effort to identify novel genes involved in recombination repair, we isolated fission yeast Schizosaccharomyces pombe mutants sensitive to methyl methanesulfonate (MMS) and a synthetic lethal with rad2.
Carr, Antony M, Furukawa, Fumiko, Iwasaki, Hiroshi, Morishita, Takashi, Sakaguchi, Chikako, Shinagawa, Hideo, Toda, Takashi   +6 more
core   +2 more sources

Cell‐free DNA aneuploidy score as a dynamic early response marker in prostate cancer

Molecular Oncology, EarlyView.
mFast‐SeqS‐based genome‐wide aneuploidy scores are concordant with aneuploidy scores obtained by whole genome sequencing from tumor tissue and can predict response to ARSI treatment at baseline and, at an early time point, to ARSI and taxanes. This assay can be easily performed at low cost and requires little input of cfDNA. Cell‐free circulating tumor
Khrystany T. Isebia, Anouk C. de Jong, Lisanne F. van Dessel, Vanja de Weerd, Corine Beaufort, Jean Helmijr, José Alberto Nakauma‐González, Job van Riet, Paul Hamberg, Daniel Vis, Michiel S. van der Heijden, Nick Beije, Martijn P. Lolkema, Teoman Deger, Saskia M. Wilting, Ronald de Wit, Maurice P. H. M. Jansen, John W. M. Martens   +17 more
wiley   +1 more source

Gene and library synthesis without amplification: polymerase step reaction (PSR)

BioTechniques, 2015
Current gene synthesis methods often incorporate a PCR amplification step in order to yield final material sufficient for resolution from multiple off-products. These amplification steps can cause stochastic sampling effects that propagate errors in gene
Zhuo-Bin Lee, Christopher Firnhaber, Jesse Clarke, Brian S. DeDecker   +3 more
doaj   +1 more source

A library of infectious hepatitis C viruses with engineered mutations in the E2 gene reveals growth-adaptive mutations that modulate interactions with scavenger receptor class B type I [PDF]

, 2016
While natural hepatitis C virus (HCV) infection results in highly diverse quasispecies of related viruses over time, mutations accumulate more slowly in tissue culture, in part because of the inefficiency of replication in cells.
Chen, Kevin, Diamond, Michael S, Evans, Matthew J, Fremont, Daved H, Luca, Vincent C, Schwarz, Megan C, Wang, David, White, James P, Zuiani, Adam   +8 more
core   +2 more sources

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

Molecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, James S. Fraser, Martin Figeac, Alexis B. Cortot, Etienne Rouleau, David Tulasne   +14 more
wiley   +1 more source

A cDNA library of the eutardigrade Hypsibius klebelsbergi Mihelčič, 1959 and analysis of the actin gene

Journal of Limnology, 2007
A cDNA library was constructed from the glacier-dwelling eutardigrade Hypsibius klebelsbergi from more than 2000 individuals collected in the Austrian Central Alps. RNA, DNA and proteins were successively isolated by the Trizol®-method.
Hartmut GREVEN, Jochen D'HAESE, Hieronymus DASTYCH, Ernst KIEHL   +3 more
doaj   +1 more source

Characterisation of a putative dothistromin biosynthetic cluster : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University, Palmerston North, New Zealand [PDF]

, 1999
The fungus Dothistroma pini is a key pathogen in New Zealand (and international) softwood plantations, most notably P. radiata. The mycotoxin dothistromin produced by this saprophytic fungus is believed to play a major role in its pathogenesis ...
Laarakkers, Seth
core  

Microbial population changes during bioremediation of nitroaromatic - and nitramine-contaminated lagoon [PDF]

, 2008
Nitration reactions of aromatic compounds are commonly involved in military industrial processes. Military industries treated their process effluents using lagoon systems for many years.
Goñi, Marisol, Merlina, Georges, Perchet, Geoffrey-Thibault, Pinelli, Eric, Revel, Jean-Claude, Sangely, Matthieu   +5 more
core   +1 more source

Respiratory complex I‐mediated NAD+ regeneration regulates cancer cell proliferation through the transcriptional and translational control of p21Cip1 expression by SIRT3 and SIRT7

Molecular Oncology, EarlyView.
NAD+ regeneration by mitochondrial complex I NADH dehydrogenase is important for cancer cell proliferation. Specifically, NAD+ is necessary for the activities of NAD+‐dependent deacetylases SIRT3 and SIRT7, which suppress the expression of p21Cip1 cyclin‐dependent kinase inhibitor, an antiproliferative molecule, at the translational and transcriptional
Masato Higurashi, Kazunori Mori, Hidetsugu Nakagawa, Momoko Uchida, Fumihiro Ishikawa, Motoko Shibanuma   +5 more
wiley   +1 more source

Construction of the suppression subtractive cDNA libraries of human large cell lung cancer line L9981 before and after transfection with nm23-H1 gene

Chinese Journal of Lung Cancer, 2008
Background and objective It has been proven that nm23-H1 gene is an important metastatic-suppressed gene of lung cancer. In order to screen the differential expression genes related to nm23-H1, we constructed the suppression subtractive cDNA libraries of
Sujuan YE, Zhihua FENG, Wen ZHU, Chunji CAI, Lu LI, Liya SUN, Haisu WAN, Li MA, Qinghua ZHOU   +8 more
doaj