Results 201 to 210 of about 1,350,251 (258)

Off-target effects as confounders of Cas13d-based lncRNA screens

Montero JJ, Trozzo R, Rad R.
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Guide RNA library representation results in gene essentiality prediction bias in genome-wide CRISPR screens

Metz P, Alves-Vasconcelos S, Wallbank R, Riepsaame J, Hassan AB.   +4 more
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Accessing Genes from Environmental DNA Libraries

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Gene discovery by ribozyme and siRNA libraries

Nature Reviews Molecular Cell Biology, 2005
Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
Hideo, Akashi, Sahohime, Matsumoto, Kazunari, Taira   +2 more
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Stalking the Gene -- DNA Libraries

New England Journal of Medicine, 1994
For molecular biologists in search of clues to cell function, the ultimate object of inquiry is the gene. A scientist looking for a particular gene faces a formidable challenge, because each cell contains more than 100,000 different genes scattered over billions of nucleotides.
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Synthetic promoter libraries – tuning of gene expression

Trends in Biotechnology, 2006
The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene knockout and strong overexpression. However, applications
Karin, Hammer, Ivan, Mijakovic, Peter Ruhdal, Jensen   +2 more
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Gene enrichment in plant genomic shotgun libraries

Current Opinion in Plant Biology, 2003
The Arabidopsis genome (about 130 Mbp) has been completely sequenced; whereas a draft sequence of the rice genome (about 430 Mbp) is now available and the sequencing of this genome will be completed in the near future. The much larger genomes of several important crop species, such as wheat (about 16,000 Mbp) or maize (about 2500 Mbp), may not be fully
Pablo D, Rabinowicz, W Richard, McCombie, Robert A, Martienssen   +2 more
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Construction of Human Naive Antibody Gene Libraries

2012
Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies.
Michael, Hust, André, Frenzel, Torsten, Meyer, Thomas, Schirrmann, Stefan, Dübel   +4 more
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Removing the Redundancy From Randomised Gene Libraries

Journal of Molecular Biology, 2003
Amino acid substitution plays a vital role in both the molecular engineering of proteins and analysis of structure-activity relationships. High-throughput substitution is achieved by codon randomisation, which generates a library of mutants (a randomised gene library) in a single experiment.
Marcus D, Hughes, David A, Nagel, Albert F, Santos, Andrew J, Sutherland, Anna V, Hine   +4 more
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Functional Gene Discovery Using Hybrid Ribozyme Libraries

2004
Hybrid ribozymes that couple the cleavage activity of hammerhead ribozymes with the unwinding activity of RNA helicases are powerful tools in the study of cell genetics and pharmaceutical drug development. They are useful for targeting a specific gene as well as screening functional genes to show phenotypic alterations.
Yoshio, Kato, Masaru, Tsunemi, Makoto, Miyagishi, Hiroaki, Kawasaki, Kazunari, Taira   +4 more
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