Results 241 to 250 of about 663,508 (269)

Construction of Human Naive Antibody Gene Libraries

2012
Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies.
Stefan Dübel, Torsten Meyer, Thomas Schirrmann, Michael Hust, André Frenzel   +4 more
openaire   +2 more sources

Gene enrichment in plant genomic shotgun libraries

Current Opinion in Plant Biology, 2003
The Arabidopsis genome (about 130 Mbp) has been completely sequenced; whereas a draft sequence of the rice genome (about 430 Mbp) is now available and the sequencing of this genome will be completed in the near future. The much larger genomes of several important crop species, such as wheat (about 16,000 Mbp) or maize (about 2500 Mbp), may not be fully
Pablo D. Rabinowicz, Robert A. Martienssen, W. Richard McCombie   +2 more
openaire   +3 more sources

Synthetic promoter libraries – tuning of gene expression

Trends in Biotechnology, 2006
The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene knockout and strong overexpression. However, applications
Karin Hammer, Ivan Mijakovic, Peter Ruhdal Jensen   +2 more
openaire   +3 more sources

Removing the Redundancy From Randomised Gene Libraries

Journal of Molecular Biology, 2003
Amino acid substitution plays a vital role in both the molecular engineering of proteins and analysis of structure-activity relationships. High-throughput substitution is achieved by codon randomisation, which generates a library of mutants (a randomised gene library) in a single experiment.
Marcus D. Hughes, Albert F. Santos, David A. Nagel, Anna V. Hine, Andrew J. Sutherland   +4 more
openaire   +3 more sources

Epitope Mapping with Gene Fragment Libraries

2001
Linear epitopes, recognized by a large number of antibodies, are easily identified by expression of small fragments of the known antigen at the tip of filamentous phage. Immobilized antibodies, incubated with such a library of peptide-displaying phage, bind selectively to the fragments containing the epitope, therefore immobilizing the peptide-phage ...
Roland E. Kontermann, Christoph H. Winter   +1 more
openaire   +2 more sources

Gene Libraries: Coverage, Efficiency and Diversity

2006
Gene libraries are a biological mechanism for generating combinatorial diversity in the immune system. However, they also bias the antibody creation process, so that they can be viewed as a way of guiding lifetime learning mechanisms. In this paper we examine the implications of this view, by examining coverage, avoidance of self, clustering and ...
Steve Cayzer, James C. Smith
openaire   +2 more sources

A vector for construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae

Plasmid, 1987
We have constructed a convenient new vector, YEp-DE, for the construction of gene libraries and the expression of heterologous genes in Saccharomyces cerevisiae. The vector contains the yeast LEU2 gene, the 2 mu origin of replication, and a region from pUC18 that includes the ampr gene, the Escherichia coli origin of replication (ori), and the LacZ ...
Muhammed Ishaq Khan, Stanley T. Crooke, Jessica A. Gorman, David J. Ecker, Tauseef R. Butt   +4 more
openaire   +3 more sources

Construction of Human Naïve Antibody Gene Libraries

2018
Size and variability often represent an obstacle in generating an effective antibody gene library for the detection of an abundant repertoire of antigens. Therefore, optimizing the construction of a large library is essential for the selection of high-affinity reactive fragments. Here, we report a highly efficient method for the construction of a human
Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Katia Scotlandi, Anna Maria Giudice   +5 more
openaire   +3 more sources

Rational design of orthogonal libraries of protein coding genes

Proceedings of the ACM Conference on Bioinformatics, Computational Biology and Biomedicine, 2012
Array-based oligonucleotide synthesis technologies provide access to thousands of custom-designed sequence variants at low cost. Large-scale synthesis and high-throughput assays have become valuable experimental tools to study in detail the interplay between sequence and function.
Daniel Ryan, Dimitris Papamichail
openaire   +3 more sources

Combinatorial Recombination of Gene Fragments to Construct a Library of Chimeras

Current Protocols in Protein Science, 2006
AbstractRecombination of distantly related and nonrelated genes is difficult using traditional PCR‐based techniques, and truncation‐based methods result in a large proportion of nonviable sequences due to frame shifts, deletions, and insertions. This unit describes a method for creating libraries of chimeras through combinatorial assembly of gene ...
Michelle M, Meyer, Kaori, Hiraga, Frances H, Arnold   +2 more
openaire   +5 more sources