Results 251 to 260 of about 1,329,302 (305)

Gene discovery by ribozyme and siRNA libraries

Nature Reviews Molecular Cell Biology, 2005
Catalytic RNAs, also known as ribozymes, can be engineered to optimize their activities in the intracellular environment. The introduction of a library of active ribozymes into cells, and the subsequent screening for phenotypic changes, allows the rapid identification of gene function.
Hideo, Akashi, Sahohime, Matsumoto, Kazunari, Taira   +2 more
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Stalking the Gene -- DNA Libraries

New England Journal of Medicine, 1994
For molecular biologists in search of clues to cell function, the ultimate object of inquiry is the gene. A scientist looking for a particular gene faces a formidable challenge, because each cell contains more than 100,000 different genes scattered over billions of nucleotides.
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Synthetic promoter libraries – tuning of gene expression

Trends in Biotechnology, 2006
The study of gene function often requires changing the expression of a gene and evaluating the consequences. In principle, the expression of any given gene can be modulated in a quasi-continuum of discrete expression levels but the traditional approaches are usually limited to two extremes: gene knockout and strong overexpression. However, applications
Karin, Hammer, Ivan, Mijakovic, Peter Ruhdal, Jensen   +2 more
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Gene enrichment in plant genomic shotgun libraries

Current Opinion in Plant Biology, 2003
The Arabidopsis genome (about 130 Mbp) has been completely sequenced; whereas a draft sequence of the rice genome (about 430 Mbp) is now available and the sequencing of this genome will be completed in the near future. The much larger genomes of several important crop species, such as wheat (about 16,000 Mbp) or maize (about 2500 Mbp), may not be fully
Pablo D, Rabinowicz, W Richard, McCombie, Robert A, Martienssen   +2 more
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Construction of Human Naive Antibody Gene Libraries

2012
Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies.
Michael, Hust, André, Frenzel, Torsten, Meyer, Thomas, Schirrmann, Stefan, Dübel   +4 more
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Removing the Redundancy From Randomised Gene Libraries

Journal of Molecular Biology, 2003
Amino acid substitution plays a vital role in both the molecular engineering of proteins and analysis of structure-activity relationships. High-throughput substitution is achieved by codon randomisation, which generates a library of mutants (a randomised gene library) in a single experiment.
Marcus D, Hughes, David A, Nagel, Albert F, Santos, Andrew J, Sutherland, Anna V, Hine   +4 more
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Functional Gene Discovery Using Hybrid Ribozyme Libraries

2004
Hybrid ribozymes that couple the cleavage activity of hammerhead ribozymes with the unwinding activity of RNA helicases are powerful tools in the study of cell genetics and pharmaceutical drug development. They are useful for targeting a specific gene as well as screening functional genes to show phenotypic alterations.
Yoshio, Kato, Masaru, Tsunemi, Makoto, Miyagishi, Hiroaki, Kawasaki, Kazunari, Taira   +4 more
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Plant Genomic Sequencing Using Gene‐Enriched Libraries

ChemInform, 2007
AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
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Construction of Human Naïve Antibody Gene Libraries

2018
Size and variability often represent an obstacle in generating an effective antibody gene library for the detection of an abundant repertoire of antigens. Therefore, optimizing the construction of a large library is essential for the selection of high-affinity reactive fragments. Here, we report a highly efficient method for the construction of a human
Michela, Pasello, Alessandra, Mallano, Michela, Flego, Silvia, Zamboni, Anna Maria, Giudice, Katia, Scotlandi   +5 more
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cDNA Libraries for Virus-Induced Gene Silencing

2010
Virus-induced gene silencing (VIGS) exploits endogenous plant antiviral defense mechanisms to posttranscriptionally silence the expression of targeted plant genes. VIGS is quick and relatively easy to perform and therefore serves as a powerful tool for high-throughput functional genomics in plants.
Andrea T, Todd, Enwu, Liu, Jonathan E, Page   +2 more
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