Results 101 to 110 of about 9,231 (134)

The HIV-1 nef gene product is associated with phosphorylation of a 46 kD cellular protein

AIDS, 1992
To study the biochemical properties of the HIV-1 nef gene product.Earlier reports suggested that Nef protein is phosphorylated and has kinase activity. These properties were examined using an in vitro translated product.A DNA fragment encoding Nef of HIV-1SF2 was transcribed in vitro under the control of T7 promoter.
L, Poulin, J A, Levy
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Purification and characterization of human immunodeficiency virus type 1 nef gene product expressed by a recombinant baculovirus

Virology, 1991
We have constructed the recombinant baculovirus which expresses the human immunodeficiency virus type 1 negative factor (nef) gene. Spodoptera frugiperda cells infected with the recombinant virus produced a 27-kDa protein which reacted with rabbit antisera raised against a carboxy-terminal synthetic peptide of the Nef protein by immunoblot analysis ...
Y, Matsuura, M, Maekawa, S, Hattori, N, Ikegami, A, Hayashi, S, Yamazaki, C, Morita, Y, Takebe   +7 more
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In Vivo Derived HIV-1nefGene Products Are Heterogeneous and Lack Detectable Nucleotide Binding Activity

AIDS Research and Human Retroviruses, 1992
Multiple HIV-1 nef genes were cloned from lymphocyte DNA of asymptomatic seropositive individuals by polymerase chain reaction (PCR). Sequence analysis of these clones revealed a unique set of nef variants with premature terminations (PCRnef 1 and 6), mutations at sites of potential posttranslational modification (PCRnef 2 and 3) and deletions.
M, Harris, S, Hislop, P, Patsilinacos, J C, Neil   +3 more
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The nef gene products of both simian and human immunodeficiency viruses enhance virus infectivity and are functionally interchangeable

Journal of Virology, 1997
Adult rhesus macaques infected with nef-defective simian immunodeficiency virus (SIV) exhibit extremely low levels of steady-state virus replication, do not succumb to immunodeficiency disease, and are protected from experimental challenge with pathogenic isolates of SIV.
E, Sinclair, P, Barbosa, M B, Feinberg
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Immunogenicity of the Human Immunodeficiency Virus (HIV) RecombinantnefGene Product. Mapping of T-Cell and B-Cell Epitopes in Immunized Chimpanzees

AIDS Research and Human Retroviruses, 1990
The nonstructural nef gene product of human immunodeficiency virus (HIV), p27, is a regulatory "early phase" protein produced by HIV-infected cells. As a possible negative regulator of transcription, it has been suggested that p27 may be involved in the control of HIV proviral latency.
E, Bahraoui, M, Yagello, J N, Billaud, J M, Sabatier, B, Guy, E, Muchmore, M, Girard, J C, Gluckman   +7 more
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Expression of the human immunodeficiency virus type 1 (HIV-1) nef gene during HIV-1 production increases progeny particle infectivity independently of gp160 or viral entry

Journal of Virology, 1995
The nef gene product of human immunodeficiency virus type 1 (HIV-1) promotes more-rapid kinetics of viral replication in primary peripheral blood mononuclear cells. We have previously shown that these enhancing effects of Nef on HIV-1 replication reflect an increase in viral infectivity detectable both in limiting dilution assays and through a single ...
M D, Miller, M T, Warmerdam, K A, Page, M B, Feinberg, W C, Greene   +4 more
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Contribution of antibody response to recombinant HIV-1 gene-encoded products nef, rev, tat, and protease in predicting development of AIDS in HIV-1-infected individuals.

Journal of acquired immune deficiency syndromes, 1991
The relation between antibody-response profiles to Escherichia coli-produced HIV-1 nef, rev, tat, and protease proteins and the risk of developing AIDS was studied using stored serum samples taken sequentially from a cohort of 195 initially symptom-free men who were seropositive for antibodies to HIV-1 structural proteins and 72 men who seroconverted ...
Reiss, P., de Wolf, F., Kuiken, C. L., de Ronde, A., Dekker, J., Boucher, C. A., Debouck, C., Lange, J. M., Goudsmit, J.   +8 more
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[Function of human immunodeficiency virus type 1 nef gene product in virus replication].

[Hokkaido igaku zasshi] The Hokkaido journal of medical science, 1996
We comparatively analyzed the replication kinetics of wild-type (wt) and nef mutant human immunodeficiency virus type 1 (HIV-1) in several CD4-positive cell lines, in order to clarify the molecular function of Nef protein. The delayed growth of nef mutant virus was observed at the initial stage of replication in all cell lines examined. This phenomenon
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The HIV-1 nef gene product

Biochemical Society Transactions, 1992
M, Harris, S, Talbot, P, Patsilinacos, J C, Neil   +3 more
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THE 27 KD PROTEIN ENCODED BY THE HIV NEF GENE SHARES STRUCTURAL AND FUNCTIONAL HOMOLOGIES WITH SEVERAL ONCOGENE PRODUCTS

1991
The HIV genome contains nine coding regions, whose function in viral replication is still partially unknown. One of these, nef (Negative factor, previously called 3'-orf or orf-B), encodes a 27 kilodalton protein (p27nef) inducing an early specific immune response in infected patients. p27nef resembles an oncogene product: (a) it is myristylated on the
GALIMI F, FERRACINI, RICCARDO, COMOGLIO PM   +2 more
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