Results 1 to 10 of about 11,888,992 (423)
Gene Targeting in Disease Networks [PDF]
Frontiers in Genetics, 2021 Profiling of whole transcriptomes has become a cornerstone of molecular biology and an invaluable tool for the characterization of clinical phenotypes and the identification of disease subtypes. Analyses of these data are becoming ever more sophisticated
Deborah Weighill +9 more
doaj +9 more sources
Gene targeting in livestock. [PDF]
Bioscientifica Proceedings, 2019 The development of nuclear transfer from tissue culture cells in livestock made it possible in principle to produce animals with subtle, directed genetic changes by in vitro modification of nuclear donor cells.
A. Thomson, M. Marques, J. Mcwhir
semanticscholar +3 more sources
Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences [PDF]
Molecular Therapy: Nucleic Acids, 2017 External guide sequence (EGS) RNAs are associated with ribonuclease P (RNase P), a tRNA processing enzyme, and represent promising agents for gene-targeting applications as they can direct RNase-P-mediated cleavage of a target mRNA.
Wei Li +9 more
doaj +4 more sources
Frontiers in Molecular Neuroscience, 2022 The striatum is involved in action selection, and its disturbance can cause movement disorders. Here, we show that leucine-rich repeats and transmembrane domain 2 (Lrtm2) controls protein sorting in striatal projection systems, and its deficiency causes ...
Misato Ichise +11 more
doaj +1 more source
Gene targeting in plants [PDF]
The EMBO Journal, 1987 Although the generation of transgenic plants is now routine, the integration of foreign genetic information has so far been at random sites in the genome. We now present evidence for directed integration into a predicted location in the host plant genome.
Augustyn Bogucki +3 more
openaire +3 more sources
CRISPR/Cas9-Mediated Multi-Allelic Gene Targeting in Sugarcane Confers Herbicide Tolerance
Frontiers in Genome Editing, 2021 Sugarcane is the source of 80% of the sugar and 26% of the bioethanol produced globally. However, its complex, highly polyploid genome (2n = 100 – 120) impedes crop improvement. Here, we report efficient and reproducible gene targeting (GT) in sugarcane,
Mehmet Tufan Oz +6 more
doaj +1 more source
The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies [PDF]
, 2013 Different regions of the bacterial 16S rRNA gene evolve at different evolutionary rates. The scientific outcome of short read sequencing studies therefore alters with the gene region sequenced.
De Vos, Paul +4 more
core +12 more sources
Cell, 2004 When it comes to silencing genes in mice, not all approaches are equal. An example published in this issue of Cell (Patrucco et al., 2004) suggests that caution should be used when validating potential drug targets by genetic disruption.
Michael D. Waterfield +3 more
openaire +3 more sources
Transcriptome Analysis of Targeted Mouse Mutations Reveals the Topography of Local Changes in Gene Expression. [PDF]
, 2016 The unintended consequences of gene targeting in mouse models have not been thoroughly studied and a more systematic analysis is needed to understand the frequency and characteristics of off-target effects.
Adkisson, Michael +9 more
core +12 more sources
Definitions, 2020 A technique that involves integration of an exogenous DNA sequence encoding a specific gene into the genome of an organism by homologous recombination.
semanticscholar +1 more source