Results 1 to 10 of about 582,689 (334)
Development of alternative gene transfer techniques for ex vivo and in vivo gene therapy in a canine model. [PDF]
Regen Ther, 2021 Gene therapy have recently attracted much attention as a curative therapeutic option for inherited single gene disorders such as hemophilia. Hemophilia is a hereditary bleeding disorder caused by the deficiency of clotting activity of factor VIII (FVIII) or factor IX (FIX), and gene therapy for hemophilia using viral vector have been vigorously ...
Noda M +11 more
europepmc +6 more sources
Gene transfer techniques for cardiac arrhythmias [PDF]
Annals of Medicine, 2004 Therapy for cardiac arrhythmias is inadequate, based on current options. Gene therapy has shown tremendous potential to investigate pathophysiology and potential therapies for cardiac diseases. The current work reviews the possibilities for application of in vivo gene transfer to treatment of common arrhythmias, including vector selection, delivery ...
J. Kevin Donahue +3 more
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Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques [PDF]
BMC Biotechnology, 2011 Background Gene therapy continues to hold great potential for treating many different types of disease and dysfunction. Safe and efficient techniques for gene transfer and expression in vivo are needed to enable gene therapeutic strategies to be ...
Daniell Henry +3 more
doaj +2 more sources
Optimal techniques for arterial gene transfer [PDF]
Cardiovascular Research, 1997 Cardiovascular gene therapy is becoming a clinical reality due to improved vectors, delivery systems and careful experimental validation studies. Nearly all cardiovascular diseases are amenable to gene therapy, but the optimal combination of vector, delivery system and therapeutic gene is likely to be unique to each application.
Laurent J. Feldman
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Optimization of human papillomavirus-based pseudovirus techniques for efficient gene transfer [PDF]
Scientific Reports, 2020 AbstractHuman papillomavirus (HPV) L1 and L2 capsid proteins self-assemble into virions capable of efficiently packaging either its 8 kilobase genome or non-viral DNA. The ability of HPV capsids to package non-viral DNA makes these a useful tool for delivering plasmids to study proteins of interest in a variety of cell types.
Timra Gilson +2 more
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A flow cytometry technique for measuring chromosome-mediated gene transfer [PDF]
Cytometry, 2001 Using artificial chromosome expression systems (ACes), we have developed a unique and rapid screening technique to quantify delivery of foreign DNA into cells in vitro. Delivery was measured within 24 h after transfection, using flow cytometry to detect the transfer of ACes labeled with thymidine analogue.
Sandra Vanderbyl +2 more
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Irreversible electroporation is a surgical ablation technique that enhances gene transfer [PDF]
Surgery, 2011 Reversible electroporation has long been used to transfer macromolecules into target cells in the laboratory by using an electric field to induce transient membrane permeability. Recently, the electric field has been modulated to produce permanent membrane permeability and cell death.
Joyce T. Au +10 more
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Zanco Journal of Pure and Applied Sciences, 2021 To increase drought resistance of efficient plant growth promoting Psuedomonas fluorescens isolated from Erbil governorate soils, exopolysaccharide (EPS) gene cluster was transferred from Rhizobium leguminosarum to P. fluorescens.
Karzan K. Mahmud, Aras Muhammad Khudhur
doaj +5 more sources
Health Science Reports, 2021 Cytomegalovirus (CMV) infection remains a major complication following allogeneic hematopoietic stem cell transplantation (HSCT). T cell response plays a critical role in inducing long‐term immunity against CMV infection/reactivation that impairs during ...
Mahshid Mehdizadeh +5 more
doaj +3 more sources
NOVEL TECHNIQUES OF GENE TRANSFER AND PLANT IMPROVEMENT: AN APPRAISAL OF TRANSFORMATION IN EUKARYOTES [PDF]
New Phytologist, 1978 SUMMARYCertain recent theoretical and technological advances make possible the development of new techniques for combining genes from diverse sources without normal sexual hybridization. These include: (1) DNA‐ and phage‐induced transformation; (2) protoplast fusion and organelle transfer; (3) gametic transformation; and (4) graft hybridization.
Kamla Kant Pandey
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