Rapid, Selection-Free, High-Efficiency Genome Editing in Protozoan Parasites Using CRISPR-Cas9 Ribonucleoproteins [PDF]
mBio, 2017 Trypanosomatids (order Kinetoplastida), including the human pathogens Trypanosoma cruzi (agent of Chagas disease), Trypanosoma brucei, (African sleeping sickness), and Leishmania (leishmaniasis), affect millions of people and animals globally. T.
Lia Carolina Soares Medeiros +8 more
doaj +5 more sources
Genome assembly and annotation of Babesia rossi, a protozoan parasite for canine babesiosis [PDF]
BMC GenomicsBackground Apicomplexan parasite, Babesia rossi, is an Ixodid tick-transmitted pathogen that causes the most severe form of canine babesiosis disease. Compared to other Babesia pathogens of dogs, B. rossi exhibits unique pathophysiology, virulence, and a
Neelam Redekar +6 more
doaj +3 more sources
Babesia gibsoni Whole-Genome Sequencing, Assembling, Annotation, and Comparative Analysis
Microbiology Spectrum, 2023 The intracellular protozoan parasite Babesia gibsoni infects canine erythrocytes and causes babesiosis. The hazards to animal health have increased due to the rise of B. gibsoni infections and medication resistance. However, the lack of high-quality full-
Qin Liu +7 more
doaj +2 more sources
Genome Assemblies across the Diverse Evolutionary Spectrum of Leishmania Protozoan Parasites [PDF]
Microbiology Resource Announcements, 2021 We report the high-quality draft assemblies and gene annotations for 13 species and/or strains of the protozoan parasite genera Leishmania, Endotrypanum, and Crithidia, which span the phylogenetic diversity of the subfamily Leishmaniinae within the ...
W. Warren +12 more
semanticscholar +4 more sources
An Extremely Streamlined Macronuclear Genome in the Free-Living Protozoan Fabrea salina
Molecular Biology and Evolution, 2022 Ciliated protists are among the oldest unicellular organisms with a heterotrophic lifestyle and share a common ancestor with Plantae. Unlike any other eukaryotes, there are two distinct nuclei in ciliates with separate germline and somatic cell functions.
Bing Zhang +6 more
semanticscholar +3 more sources
Genome-Wide Libraries for Protozoan Pathogen Drug Target Screening Using Yeast Surface Display
ACS Infectious Diseases, 2022 The lack of genetic tools to manipulate protozoan pathogens has limited the use of genome-wide approaches to identify drug or vaccine targets and understand these organisms’ biology.
Rhiannon T Heslop +6 more
semanticscholar +4 more sources
PLoS ONE, 2022 Plasmodium falciparum, a protozoan parasite and causative agent of human malaria, has one of the most A/T-biased genomes sequenced to date. This may give the genome and the transcriptome unusual structural features.
Franck Dumetz +4 more
doaj +2 more sources
A chromosome-scale genome assembly of Giardia duodenalis by long-read sequencing of ten trophozoites [PDF]
Scientific DataGiardia duodenalis, a protozoan parasite of important zoonotic concern, is estimated to cause approximately 280 million human infections annually worldwide. Currently, genome sequencing of G.
Jingjing Sun +9 more
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Chromosome-level genome assembly of Trichomonas vaginalis strain IR-78 (ATCC 50138) [PDF]
Scientific DataTrichomonas vaginalis is a flagellated protozoan parasite and the causative agent of trichomoniasis, the most prevalent non-viral sexually transmitted infection, with significant impact on public health and economy.
Mostafa Y. Abdel-Glil +3 more
doaj +2 more sources
Genome Medicine, 2019 A major advance in antimalarial drug discovery has been the shift towards cell-based phenotypic screening, with notable progress in the screening of compounds against the asexual blood stage, liver stage, and gametocytes. A primary method for drug target
Annie N. Cowell, Elizabeth A. Winzeler
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