Results 11 to 20 of about 70 (69)

Nanopore Sequencing Significantly Improves Genome Assembly of the Protozoan Parasite Trypanosoma cruzi [PDF]

Genome Biology and Evolution, 2019
Abstract Chagas disease was described by Carlos Chagas, who first identified the parasite Trypanosoma cruzi from a 2-year-old girl called Berenice. Many T. cruzi sequencing projects based on short reads have demonstrated that genome assembly and downstream comparative analyses are extremely challenging in this species, given that half of
Gregorio Iraola, Gregorio Iraola, Florencia Díaz-Viraqué, Gonzalo Greif, Carlos Robello, Carlos Robello, Rita de Cássia Moreira de Souza, Sebastián Pita, Sebastián Pita   +8 more
openaire   +4 more sources

CRISPR/Cas9 Ribonucleoprotein-Based Genome Editing Methodology in the Marine Protozoan Parasite Perkinsus marinus [PDF]

Frontiers in Bioengineering and Biotechnology, 2021
Perkinsus marinus (Perkinsozoa), a close relative of apicomplexans, is an osmotrophic facultative intracellular marine protozoan parasite responsible for “Dermo” disease in oysters and clams. Although there is no clinical evidence of this parasite infecting humans, HLA-DR40 transgenic mice studies strongly suggest the parasite as a natural adjuvant in ...
Adrienne N. Tracy, Adrienne N. Tracy, Kousuke Umeda, Kousuke Umeda, Asha V Sidhu, Asha V Sidhu, José A. Fernández Robledo, Derek E Hernández, Derek E Hernández, Hannah A Waugh, Hannah A Waugh, Raghavendra Yadavalli   +11 more
openaire   +4 more sources

Telomeric localization of the modified DNA base J in the genome of the protozoan parasite Leishmania [PDF]

Nucleic Acids Research, 2007
Base J or beta-d-glucosylhydroxymethyluracil is a DNA modification replacing a fraction of thymine in the nuclear DNA of kinetoplastid parasites and of Euglena. J is located in the telomeric sequences of Trypanosoma brucei and in other simple repeat DNA sequences.
Paul-André Genest, Piet Borst, Henri G.A.M. van Luenen, Bas ter Riet, Tony Cijsouw   +4 more
openaire   +3 more sources

The genome of the protozoan parasite Cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates [PDF]

International Journal for Parasitology, 2017
Vaccine development targeting protozoan parasites remains challenging, partly due to the complex interactions between these eukaryotes and the host immune system. Reverse vaccinology is a promising approach for direct screening of genome sequence assemblies for new vaccine candidate proteins.
Nicola Palmieri, Damer P. Blake, Aruna Shrestha, Bärbel Ruttkowski, Anja Joachim, Tomas Beck, Fiona M. Tomley, Claus Vogl   +7 more
openaire   +3 more sources

The compact genome of Giardia muris reveals important steps in the evolution of intestinal protozoan parasites [PDF]

Microbial Genomics, 2019
Diplomonad parasites of the genus Giardia have adapted to colonizing different hosts, most notably the intestinal tract of mammals. The human-pathogenic Giardia species, Giardia intestinalis, has been extensively studied at the genome and gene expression level, but no such information is available for other Giardia species.
Ásgeir Ástvaldsson, Ásgeir Ástvaldsson, Feifei Xu, Alejandro Jiménez-González, Jan Andersson, Jon Jerlström-Hultqvist, Elin Einarsson, Elin Einarsson, Lars Eckmann, Dimitra Peirasmaki, Dimitra Peirasmaki, Staffan G. Svärd   +11 more
openaire   +3 more sources

Microglial IKKβ Alters Central and Peripheral Immune Activity at Distinct Time Points After Spinal Cord Injury

Glia, EarlyView.
Microglial NF‐κB signaling increases M1‐like spinal microglia over time after spinal cord injury. Microglial NF‐κB signaling broadly affects gene expression in spinal cord. Microglial NF‐κB signaling modulates splenocytes acutely after spinal cord injury.
Micaela L. O'Reilly, Mariah J. Wulf, Theresa M. Connors, Ying Jin, Frank Bearoff, Julien Bouyer, Sandhya Kortagere, John R. Bethea, Veronica J. Tom   +8 more
wiley   +1 more source

RNA Editing and Mitochondrial Genomic Organization in the Cryptobiid Kinetoplastid Protozoan Trypanoplasma borreli [PDF]

Molecular and Cellular Biology, 1994
The bodonids and cryptobiids represent an early diverged sister group to the trypanosomatids among the kinetoplastid protozoa. The trypanosome type of uridine insertion-deletion RNA editing was found to occur in the cryptobiid fish parasite Trypanoplasma borreli.
Larry Simpson, Dmitri A. Maslov
openaire   +3 more sources

Development and Validation of In‐House Conventional and Multiplex PCR Methods for the Detection and Identification of Lophomonas spp.: An Innovative Approach

Journal of Clinical Laboratory Analysis, EarlyView.
Sequence alignment of the SSU rRNA of the two Lophomonas species, L. blattarum and L. striata. The red rectangle highlights the position of the reverse universal primer; the yellow, the L. blattarum‐specific forward primer; and the green, the L. striata‐specific forward primer used in multiplex PCR.
Maryam Nakhaei, Mahdi Fakhar, Abouzar Bagheri, Hajar Ziaei Hezarjaribi, Saied Abediankenari, Ali Sharifpour, Maryam Ghasemi   +6 more
wiley   +1 more source

Calpain Proteases and the Evolving Signaling Network in Insect Embryonic Patterning

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, EarlyView.
Insect embryonic Dorsal‐Ventral (DV) patterning relies on the BMP and Toll pathways to different extents. Calcium‐dependent cystein proteases of the Calpain family also exert an important function to pattern the DV axis. In Drosophila, Calpain A cleaves the Cactus/IkappaB inhibitor and modifies Toll signals in ventral regions of the embryo. In Rhodnius
Alison Julio, Helena Araujo
wiley   +1 more source

Genome-wide libraries for protozoan pathogens drug target screening using yeast surface display

ACS Infectious Diseases, 2022
AbstractThe lack of genetic tools to manipulate protozoan pathogens has limited the use of genome-wide approaches to identify drug or vaccine targets and understand these organisms’ biology. We have developed an efficient method to construct genome-wide libraries for yeast surface display (YSD) and developed a YSD fitness screen (YSD-FS) to identify ...
Rhiannon Heslop, Mengjin Gao, Andressa Brito Lira, Tamara Sternlieb, Mira Loock, Sahil Rao Sanghi, Igor Cestari   +6 more
openaire   +3 more sources