Results 31 to 40 of about 24,640 (259)

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

, 2009
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD, Veenendaal, A., Johan T den Dunnen, Martien AM Groenen, Bert W Dibbits, Thomas FC Chin-A-Woeng, Dunnen, J.T., den, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Chin-A-Woeng, T.F.C., Dibbits Bert W, Hindrik HD Kerstens, Richard PMA Crooijmans, Veenendaal Albertine, den Dunnen Johan T, Chin-A-Woeng Thomas FC, Crooijmans, R.P.M.A., Albertine Veenendaal, Groenen Martien AM, Crooijmans Richard PMA   +20 more
core   +1 more source

Tigmint: correcting assembly errors using linked reads from large molecules

BMC Bioinformatics, 2018
Background Genome sequencing yields the sequence of many short snippets of DNA (reads) from a genome. Genome assembly attempts to reconstruct the original genome from which these reads were derived.
Shaun D. Jackman, Lauren Coombe, Justin Chu, Rene L. Warren, Benjamin P. Vandervalk, Sarah Yeo, Zhuyi Xue, Hamid Mohamadi, Joerg Bohlmann, Steven J.M. Jones, Inanc Birol   +10 more
doaj   +1 more source

Enhancement of de novo sequencing, assembly and annotation of the Mongolian gerbil genome with transcriptome sequencing and assembly from several different tissues

BMC Genomics, 2019
Background The Mongolian gerbil (Meriones unguiculatus) has historically been used as a model organism for the auditory and visual systems, stroke/ischemia, epilepsy and aging related research since 1935 when laboratory gerbils were separated from their ...
Shifeng Cheng, Yuan Fu, Yaolei Zhang, Wenfei Xian, Hongli Wang, Benedikt Grothe, Xin Liu, Xun Xu, Achim Klug, Elizabeth A. McCullagh   +9 more
doaj   +1 more source

Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting. [PDF]

, 2014
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infection. Whole-genome sequencing of MRSA has been used to define phylogeny and transmission in well-resourced healthcare settings, yet the greatest burden of nosocomial ...
Thaipadungpanit, J, Holden, MTG, Cauchemez, S, Jombart, Thibaut, Nickerson, Emma K, Hongsuwan, M, Cori, A, Cauchemez, Simon, Holden, Matthew T G, Parkhill, J, Tong, SYC, Nickerson, EK, Day, Nicholas P, Day, NP, Fraser, C, Tong, Steven Y C, Fraser, Christophe, Limmathurotsakul, Direk, Limmathurotsakul, D, Wuthiekanun, Vanaporn, Peacock, SJ, Köser, Claudio U, Wuthiekanun, V, Cooper, BS, Cori, Anne, Parkhill, Julian, Cooper, Ben S, Thaipadungpanit, Janjira, Peacock, Sharon J, Jombart, T, Hongsuwan, Maliwan, Holden, Matthew T G; id_orcid, Koser, CU   +32 more
core   +2 more sources

Genome Sequencing and Assembly Strategies and a Comparative Analysis of the Genomic Characteristics in Penaeid Shrimp Species

Frontiers in Genetics, 2021
Penaeid shrimp (family Penaeidae) represents one of the most economically and ecologically important groups of crustaceans. However, their genome sequencing and assembly have encountered extreme difficulties during the last 20 years. In this study, based
Jianbo Yuan, Jianbo Yuan, Jianbo Yuan, Xiaojun Zhang, Xiaojun Zhang, Xiaojun Zhang, Fuhua Li, Fuhua Li, Fuhua Li, Jianhai Xiang, Jianhai Xiang, Jianhai Xiang   +11 more
doaj   +1 more source

High-speed whole-genome sequencing of a Whippet: Rapid chromosome-level assembly and annotation of an extremely fast dog’s genome

GigaByte
The time required for genome sequencing and de novo assembly depends on the interaction between laboratory work, sequencing capacity, and the bioinformatics workflow, often constrained by external sequencing services.
Marcel Nebenführ , David Prochotta , Alexander Ben Hamadou , Axel Janke , Charlotte Gerheim , Christian Betz , Carola Greve , Hanno Jörn Bolz   +7 more
doaj   +1 more source

High-Quality de novo Chromosome-Level Genome Assembly of a Single Bombyx mori With BmNPV Resistance by a Combination of PacBio Long-Read Sequencing, Illumina Short-Read Sequencing, and Hi-C Sequencing

Frontiers in Genetics, 2021
The reference genomes of Bombyx mori (B. mori), Silkworm Knowledge-based database (SilkDB) and SilkBase, have served as the gold standard for nearly two decades.
Min Tang, Suqun He, Xun Gong, Xun Gong, Peng Lü, Rehab H. Taha, Keping Chen   +6 more
doaj   +1 more source

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Completion of draft bacterial genomes by long-read sequencing of synthetic genomic pools

BMC Genomics, 2020
Background Illumina technology currently dominates bacterial genomics due to its high read accuracy and low sequencing cost. However, the incompleteness of draft genomes generated by Illumina reads limits their application in comprehensive genomics ...
Hooman Derakhshani, Steve P. Bernier, Victoria A. Marko, Michael G. Surette   +3 more
doaj   +1 more source

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source