Results 31 to 40 of about 306,835 (260)

Completion of draft bacterial genomes by long-read sequencing of synthetic genomic pools

BMC Genomics, 2020
Background Illumina technology currently dominates bacterial genomics due to its high read accuracy and low sequencing cost. However, the incompleteness of draft genomes generated by Illumina reads limits their application in comprehensive genomics ...
Hooman Derakhshani, Steve P. Bernier, Victoria A. Marko, Michael G. Surette   +3 more
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

A high quality assembly of the Nile Tilapia (Oreochromis niloticus) genome reveals the structure of two sex determination regions

BMC Genomics, 2017
Background Tilapias are the second most farmed fishes in the world and a sustainable source of food. Like many other fish, tilapias are sexually dimorphic and sex is a commercially important trait in these fish.
Matthew A. Conte, William J. Gammerdinger, Kerry L. Bartie, David J. Penman, Thomas D. Kocher   +4 more
doaj   +1 more source

The challenge of genome sequence assembly [PDF]

The Open Bioinformatics Journal, 2018
Background: Although whole genome sequencing is enabling numerous advances in many fields achieving complete chromosome-level sequence assemblies for diverse species presents difficulties. The problems in part reflect the limitations of current sequencing technologies. Chromosome assembly from ‘short read’ sequence data is confounded by the presence of
openaire   +4 more sources

Limitations of next-generation genome sequence assembly [PDF]

Nature Methods, 2010
High-throughput sequencing technologies promise to transform the fields of genetics and comparative biology by delivering tens of thousands of genomes in the near future. Although it is feasible to construct de novo genome assemblies in a few months, there has been relatively little attention to what is lost by sole application of short sequence reads.
Can, Alkan, Saba, Sajjadian, Evan E, Eichler   +2 more
openaire   +2 more sources

Somatic mutational landscape in von Hippel–Lindau familial hemangioblastoma

Molecular Oncology, EarlyView.
The causes of central nervous system (CNS) hemangioblastoma in Von Hippel–Lindau (vHL) disease are unclear. We used Whole Exome Sequencing (WES) on familial hemangioblastoma to investigate events that underlie tumor development. Our findings suggest that VHL loss creates a permissive environment for tumor formation, while additional alterations ...
Maja Dembic, Anne Nørremølle, Lilian Bomme Ousager, Lars van Brakel Andersen, Marie Louise Mølgaard Binderup, Mads Thomassen   +5 more
wiley   +1 more source

Comparison of the DNBSEQ platform and Illumina HiSeq 2000 for bacterial genome assembly

Scientific Reports
The Illumina HiSeq platform has been a commonly used option for bacterial genome sequencing. Now the BGI DNA nanoball (DNB) nanoarrays platform may provide an alternative platform for sequencing of bacterial genomes.
Tongyuan Hu, Jianwei Chen, Xiaoqian Lin, Wenxin He, Hewei Liang, Mengmeng Wang, Wenxi Li, Zhinan Wu, Mo Han, Xin Jin, Karsten Kristiansen, Liang Xiao, Yuanqiang Zou   +12 more
doaj   +1 more source

PacBio But Not Illumina Technology Can Achieve Fast, Accurate and Complete Closure of the High GC, Complex Burkholderia pseudomallei Two-Chromosome Genome

Frontiers in Microbiology, 2017
Although PacBio third-generation sequencers have improved the read lengths of genome sequencing which facilitates the assembly of complete genomes, no study has reported success in using PacBio data alone to completely sequence a two-chromosome bacterial
Jade L. L. Teng, Jade L. L. Teng, Jade L. L. Teng, Jade L. L. Teng, Man Lung Yeung, Man Lung Yeung, Man Lung Yeung, Man Lung Yeung, Elaine Chan, Lilong Jia, Chi Ho Lin, Yi Huang, Herman Tse, Herman Tse, Herman Tse, Herman Tse, Samson S. Y. Wong, Samson S. Y. Wong, Samson S. Y. Wong, Samson S. Y. Wong, Pak Chung Sham, Pak Chung Sham, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo   +31 more
doaj   +1 more source

Effects of GC bias in next-generation-sequencing data on de novo genome assembly. [PDF]

PLoS ONE, 2013
Next-generation-sequencing (NGS) has revolutionized the field of genome assembly because of its much higher data throughput and much lower cost compared with traditional Sanger sequencing. However, NGS poses new computational challenges to de novo genome
Yen-Chun Chen, Tsunglin Liu, Chun-Hui Yu, Tzen-Yuh Chiang, Chi-Chuan Hwang   +4 more
doaj   +1 more source

Sequencing and assembling bear genomes: the bare necessities

Frontiers in Zoology, 2022
AbstractUnique genetic adaptations are present in bears of every species across the world. From (nearly) shutting down important organs during hibernation to preventing harm from lifestyles that could easily cause metabolic diseases in humans, bears may hold the answer to various human ailments.
Courtney Willey, Ron Korstanje
openaire   +4 more sources