Results 51 to 60 of about 24,640 (259)

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres [PDF]

, 2011
Background: Pyrenophora teres f. teres is a necrotrophic fungal pathogen and the cause of one of barley's most important diseases, net form of net blotch.
Ellwood, S.R., Syme, R.A., Liu, Z.H., Friesen, T.L., Moffat, C.S., Oliver, R.P., Keiper, F., Lai, Z.B., Hane, J.K.   +8 more
core  

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

PacBio But Not Illumina Technology Can Achieve Fast, Accurate and Complete Closure of the High GC, Complex Burkholderia pseudomallei Two-Chromosome Genome

Frontiers in Microbiology, 2017
Although PacBio third-generation sequencers have improved the read lengths of genome sequencing which facilitates the assembly of complete genomes, no study has reported success in using PacBio data alone to completely sequence a two-chromosome bacterial
Jade L. L. Teng, Jade L. L. Teng, Jade L. L. Teng, Jade L. L. Teng, Man Lung Yeung, Man Lung Yeung, Man Lung Yeung, Man Lung Yeung, Elaine Chan, Lilong Jia, Chi Ho Lin, Yi Huang, Herman Tse, Herman Tse, Herman Tse, Herman Tse, Samson S. Y. Wong, Samson S. Y. Wong, Samson S. Y. Wong, Samson S. Y. Wong, Pak Chung Sham, Pak Chung Sham, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Susanna K. P. Lau, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo, Patrick C. Y. Woo   +31 more
doaj   +1 more source

Recent Advances in Assembly of Complex Plant Genomes

Genomics, Proteomics & Bioinformatics, 2023
Over the past 20 years, tremendous advances in sequencing technologies and computational algorithms have spurred plant genomic research into a thriving era with hundreds of genomes decoded already, ranging from those of nonvascular plants to those of ...
Weilong Kong, Yibin Wang, Shengcheng Zhang, Jiaxin Yu, Xingtan Zhang   +4 more
doaj   +1 more source

Limitations of next-generation genome sequence assembly [PDF]

Nature Methods, 2010
High-throughput sequencing technologies promise to transform the fields of genetics and comparative biology by delivering tens of thousands of genomes in the near future. Although it is feasible to construct de novo genome assemblies in a few months, there has been relatively little attention to what is lost by sole application of short sequence reads.
Can, Alkan, Saba, Sajjadian, Evan E, Eichler   +2 more
openaire   +2 more sources

Mitochondrial translation and coordinated cytosolic translation in metabolic homeostasis and disease

FEBS Letters, EarlyView.
Mitochondrial function depends on coordinated protein production from the nuclear and mitochondrial genomes. This review highlights how cytosolic and mitochondrial translation, mRNA localization, protein import, and respiratory complex assembly are integrated to maintain mitochondrial proteostasis and function.
Boseon Kim, Thi Thanh Mai Tran, Nakyung Kang, Min‐Woo Lee, Mihye Lee   +4 more
wiley   +1 more source

Bioinformatics' approaches to detect genetic variation in whole genome sequencing data [PDF]

, 2010
Current genetic marker repositories are not sufficient or even are completely lacking for most farm animals. However, genetic markers are essential for the development of a research tool facilitating discovery of genetic factors that contribute to ...
Kerstens, H.H.D.
core  

Leveraging the Assembly of the Durum Wheat Genome cv. Svevo

, 2017
Tetraploid wheat species, with his two wild and cultivated subspecies T. turgidum ssp. dicoccoides and ssp. durum, is a cornerstone of the wheat polyploidy complex.
The International Durum Wheat Sequencing Consortium, The International Durum wheat Genome Sequencing Consortium, L. Cattivelli, M. Maccaferri   +3 more
core   +1 more source

Genome Sequencing and Assembly by Long Reads in Plants [PDF]

Genes, 2017
Plant genomes generated by Sanger and Next Generation Sequencing (NGS) have provided insight into species diversity and evolution. However, Sanger sequencing is limited in its applications due to high cost, labor intensity, and low throughput, while NGS reads are too short to resolve abundant repeats and polyploidy, leading to incomplete or ambiguous ...
Changsheng Li, Feng Lin, Dong An, Wenqin Wang, Ruidong Huang   +4 more
openaire   +2 more sources