Results 41 to 50 of about 24,640 (259)

Optical mapping as a routine tool for bacterial genome sequence finishing [PDF]

, 2007
Background: In sequencing the genomes of two Xenorhabdus species, we encountered a large number of sequence repeats and assembly anomalies that stalled finishing efforts.
Norton, S., Miller, N., Zijin Du, Goldman, Barry S., Latreille Phil, Bode, Helge Björn, Henkhaus John, Forst, Steve, John Henkhaus, Goodrich-Blair, H., Miller, Nancy, Slater Steven, Stacie Norton, Bode, H., Goldman Barry S, Henkhaus, J., Gaudriault, S., Steve Forst, Creg Darby, Gaudriault Sophie, Darby Creg, Goldman, B., Gaudriault, Sophie, Darby, C., Du, Z., Goodner, B., Norton, Stacie, Sophie Gaudriault, Miller Nancy, Goodrich-Blair, Heidi, Henkhaus, John, Heidi Goodrich-Blair, Gaudriault, Sophie, S., Latreille, Phil, Slater, S., Goodrich-Blair Heidi, Goodner, Brad, Goodner Brad, Latreille, P., Bode, Helge B., Nancy Miller, Norton Stacie, Brad Barbazuk, Phil Latreille, Steven Slater, Slater, Steven, Forst Steve, Helge B Bode, Forst, S., Barbazuk, Brad, Barbazuk Brad, Du, Zijin, Bode Helge B, Barry S Goldman, Du Zijin, Brad Goodner, Barbazuk, B., Darby, Creg   +57 more
core   +1 more source

Whole genome amplification and de novo assembly of single bacterial cells.

PLoS ONE, 2009
BackgroundSingle-cell genome sequencing has the potential to allow the in-depth exploration of the vast genetic diversity found in uncultured microbes.
Sébastien Rodrigue, Rex R Malmstrom, Aaron M Berlin, Bruce W Birren, Matthew R Henn, Sallie W Chisholm   +5 more
doaj   +1 more source

Effects of GC bias in next-generation-sequencing data on de novo genome assembly. [PDF]

PLoS ONE, 2013
Next-generation-sequencing (NGS) has revolutionized the field of genome assembly because of its much higher data throughput and much lower cost compared with traditional Sanger sequencing. However, NGS poses new computational challenges to de novo genome
Yen-Chun Chen, Tsunglin Liu, Chun-Hui Yu, Tzen-Yuh Chiang, Chi-Chuan Hwang   +4 more
doaj   +1 more source

A context‐dependent modulatory role for eIF6 in acquired resistance to vemurafenib in melanoma

FEBS Letters, EarlyView.
Acquired resistance to vemurafenib upregulates the translation factor eIF6 in melanoma cells. Silencing eIF6 in resistant cells reduces proliferation and partially restores drug sensitivity, whereas its overexpression increases sensitivity across melanoma lines regardless of BRAF status, via modulation of mTOR, S6K, and MAPK signaling.
George Kyriakopoulos, Antonia Petropoulou, Alexandra Anastogianni, Angelina Bania, Vassilis Stamatakis, Argyris Alexiou, Chi‐Ping Day, Glenn Merlino, Katerina Grafanaki, Constantinos Stathopoulos   +9 more
wiley   +1 more source

Genome sequencing of Pectinophora gossypiella 4th instar female alternate pseudohaplotype

, 2022
Genome sequencing and assembly of Pectinophora gossypiella (Pink bollworm) was conducted by the USDA-ARS Ag100Pest ...
USDA Ag100 Pest (17857382), HudsonAlpha Genome Sequencing Center (17856551), United States Department of Agriculture (17854922)   +2 more
core  

The challenge of genome sequence assembly [PDF]

The Open Bioinformatics Journal, 2018
Background: Although whole genome sequencing is enabling numerous advances in many fields achieving complete chromosome-level sequence assemblies for diverse species presents difficulties. The problems in part reflect the limitations of current sequencing technologies. Chromosome assembly from ‘short read’ sequence data is confounded by the presence of
openaire   +4 more sources

Comparison of the DNBSEQ platform and Illumina HiSeq 2000 for bacterial genome assembly

Scientific Reports
The Illumina HiSeq platform has been a commonly used option for bacterial genome sequencing. Now the BGI DNA nanoball (DNB) nanoarrays platform may provide an alternative platform for sequencing of bacterial genomes.
Tongyuan Hu, Jianwei Chen, Xiaoqian Lin, Wenxin He, Hewei Liang, Mengmeng Wang, Wenxi Li, Zhinan Wu, Mo Han, Xin Jin, Karsten Kristiansen, Liang Xiao, Yuanqiang Zou   +12 more
doaj   +1 more source

A high quality assembly of the Nile Tilapia (Oreochromis niloticus) genome reveals the structure of two sex determination regions

BMC Genomics, 2017
Background Tilapias are the second most farmed fishes in the world and a sustainable source of food. Like many other fish, tilapias are sexually dimorphic and sex is a commercially important trait in these fish.
Matthew A. Conte, William J. Gammerdinger, Kerry L. Bartie, David J. Penman, Thomas D. Kocher   +4 more
doaj   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

Changthangi Pashmina Goat Genome: Sequencing, Assembly, and Annotation

Frontiers in Genetics, 2021
Pashmina goats produce the world's finest and the most costly animal fiber (Pashmina) with an average fineness of 11–13 microns and have more evolved mechanisms than any known goat breed around the globe. Despite the repute of Pashmina goat for producing
Basharat Bhat, Nazir A. Ganai, Ashutosh Singh, Rakeeb Mir, Syed Mudasir Ahmad, Sajad Majeed Zargar, Firdose Malik   +6 more
doaj   +1 more source