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Current Protocols in Plant Biology, 2017
AbstractGenotyping‐by‐sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high‐throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost ...
Jason G, Wallace, Sharon E, Mitchell
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AbstractGenotyping‐by‐sequencing (GBS) refers to a suite of related methods that obtain genotype data from samples by using restriction enzyme digestion followed by high‐throughput sequencing. GBS is a refinement of restriction site–associated DNA sequencing (RADseq) methods, with a goal of being able to perform library preparations quickly, cost ...
Jason G, Wallace, Sharon E, Mitchell
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Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny [PDF]
, 2020 Genotyping-by-sequencing (GBS) is a method to discover and genotype simultaneous genome-wide high-throughput single nucleotide polymorphisms (SNPs). GBS is based on reducing genome complexity with restriction enzymes. Here we describe a method developed by Elshire et al.
Favre, Félicien +3 more
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Skim-Based Genotyping by Sequencing
2014Genotyping by sequencing (GBS) is a relatively new method used to determine the differences in the genetic makeup of individuals. Its novelty stems from a combination of two already available methods: genotyping and next-generation sequencing. Depending on the individual study design GBS protocols can take multiple forms, however most share a sequence ...
Golicz, Agnieszka A. +2 more
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Methods for Genotyping-by-Sequencing
2016A major goal for biologists is to understand the connection between genes and phenotypic traits, and genetic mapping in experimental populations remains a powerful approach for discovering the causal genes underlying phenotypes. For genetic mapping, the process of genotyping was previously a major rate-limiting step.
Rowan, B. +4 more
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Multiplexed ISSR Genotyping by Sequencing (MIG-Seq)
2023Multiplexed inter-simple sequence repeat (ISSR) genotyping by sequencing (MIG-seq) is a simple, rapid, and inexpensive method for detecting single-nucleotide polymorphisms (SNPs) using next-generation sequencing (NGS). The advantages of MIG-seq include easy application to various species without prior genetic information. In addition, this method opens
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Genotyping by sequencing for SNP marker development in onion
Genome, 2020Onion (Allium cepa) is not highly tractable for development of molecular markers due to its large (16 gigabases per 1C) nuclear genome. Single nucleotide polymorphisms (SNPs) are useful for genetic characterization and marker-aided selection of onion because of codominance and common occurrence in elite germplasm. We completed genotyping by sequencing
Labate, Joanne A. +2 more
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Genotyping-by-Sequencing in Potato
2017Genotyping-by-sequencing (GBS) is one of the most cost-effective approaches to sequencing in potato (SNP) discovery and genotyping. The reduction of genome complexity that is central to the GBS approach is useful in the analysis of many plant genomes in which large size and polyploidy can prove challenging.
Chiheb Boudhrioua +6 more
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High Resolution MICA Genotyping by Sequence-Based Typing (SBT)
2012We have developed a MICA typing method based on polymerase chain reaction (PCR) sequence-based typing and a computer program that determines the polymorphisms and distinguishes the GCT repeats in exon 5. One PCR amplification was performed to obtain templates of 2.2 kb, including exons 2, 3, 4, and 5 of MICA to be sequenced with two forward and two ...
Yizhou, Zou, Peter, Stastny
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Analysis of chrysanthemum genetic diversity by genotyping-by-sequencing
Horticulture, Environment, and Biotechnology, 2020Application of next-generation sequencing to very large genomes, such as chrysanthemum (Chrysanthemum sp.), faces challenges including repeated sequences, limited short-reads, and various contigs in massive fragments. Genotyping-by-sequencing (GBS) can overcome many of these drawbacks by high-multiplexing, and potential genotyping-sequencing approaches
Toan Khac Nguyen +2 more
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Escaneo científico y tecnológico Genotyping by sequencing
2020El objetivo de este ejercicio de inteligencia estratégica para la I+D+i, se enfoca en la identificación a través de un escaneo científico y un escaneo tecnológico del estado actual de uso con fines de investigación y comercialización de la técnica de genotipado por secuenciación (Genotyping by Sequencing – GBS) tanto en publicación científicas en la ...
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