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Derivation and characterization of pluripotent embryonic germ cells in chicken

Molecular Reproduction and Development, 2000
Embryonic germ (EG) cell lines established from primordial germ cells (PGCs) are undifferentiated and pluripotent stem cells. To date, EG cells with proven germ-line transmission have been completely established only in the mouse with embryonic stem (ES) cells.
T S, Park, J Y, Han
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[Embryonal germ cells and germ cell tumors].

Verhandlungen der Deutschen Gesellschaft fur Pathologie, 2008
Testicular germ cell tumors comprise of group of pluripotent tumors including seminomas and nonseminomas, arise from intratubular germ cell neoplasia and originate from the primordial germ cells/ gonocytes. Many well characterized markers of embryonic stem cells including CD9, PODXL and centromere-specific histone-H3-like protein CENPA are consistently
K, Biermann   +14 more
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[Germ cell and embryonal tumors].

Der Radiologe, 2015
Germ cell tumors, which constitute approximately 3-5% of tumors of the central nervous system (CNS), can be subdivided into germinomas, embryonal carcinomas, yolk sac tumors, choriocarcinomas, teratomas and mixed germ cell tumors. The diagnosis of intracranial germ cell tumor is based on the clinical symptoms, detection of tumor markers, such as alpha ...
W, Reith   +3 more
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Human Embryonic Stem Cells and Germ Cell Development

2009
Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of blastocysts and are characterized by the ability to differentiate into the three primary germ layers. Evidence shows, however, that the cells of the ICM and derived ESCs are not identical.
Nina J. Kossack   +2 more
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Immunomagnetic Isolation of Primordial Germ Cells and the Establishment of Embryonic Germ Cell Lines in the Mouse

Cloning, 1999
The stage-specific embryonic antigen 1 (SSEA-1) is a cell marker of primordial germ cells (PGCs). In the present study, it is shown that isolation and purification of PGCs from 8.5-11.5 days post coitum (dpc) embryos can be achieved by a immunomagnetic cell sorting method using SSEA-1 antibody-conjugated magnetic beads, and then the sorted PGCs can be ...
G, Durcova-Hills   +5 more
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Differentiation and characterization of embryonic stem cells into three germ layers

Biochemical and Biophysical Research Communications, 2009
Embryonic stem cells differentiated on M15 cells have previously been shown to give rise to cells of the mesendodermal and definitive endodermal lineages. Here we demonstrate that neuroectodermal and mesodermal lineages can be derived from ES cells cultured on M15 cells and subsequently subjected to specific culture conditions, as confirmed by the ...
Shiraki N   +7 more
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Nuclear proteome analysis of undifferentiated mouse embryonic stem and germ cells

ELECTROPHORESIS, 2008
AbstractEmbryonic stem cells (ESCs) and embryonic germ cells (EGCs) provide exciting models for understanding the underlying mechanisms that make a cell pluripotent. Indeed, such understanding would enable dedifferentiation and reprogrammation of any cell type from a patient needing a cell therapy treatment.
Buhr, Nicolas   +5 more
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Embryonic germ cell lines and their derivation from mouse primordial germ cells.

Ciba Foundation symposium, 1995
When primordial germ cells of the mouse are cultured on feeder layers with the addition of the polypeptide signalling molecules leukaemia inhibitory factor, Steel factor and basic fibroblast growth factor they give rise to cells that resemble undifferentiated blastocyst-derived embryonic stem cells.
P A, Labosky, D P, Barlow, B L, Hogan
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Fibroblast Growth Factor-Mediated Growth Regulation and Receptor Expression in Embryonal Carcinoma and Embryonic Stem Cells and Human Germ Cell Tumors

Biochemical and Biophysical Research Communications, 1993
FGFs have been implicated in the induction of mesoderm in amphibian development and are present in the mouse embryo at stages that would be appropriate for a similar function in mammals. Primitive ectoderm would then be the target tissue. We have now changes in the expression of receptors for FGFs during the differentiation of embryonal carcinoma (EC ...
Mummery, C.L.   +5 more
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Effects of Irradiation on Germ Cells and Embryonic Development in Teleosts

1979
Publisher Summary This chapter discusses the effects of irradiation on germ cells and embryonic development in teleosts. Sterility and weight reduction of testes after irradiation are mainly because of actual cell loss, and the order of decreasing sensitivity of spermatogenic cells is from the most sensitive spermatogonia, through primary and ...
N, Egami, K I, Ijiru
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