Results 261 to 270 of about 12,778 (301)

Derivation of embryonic germ cells and male gametes from embryonic stem cells

Nature, 2003
Egg and sperm cells (gametes) of the mouse are derived from a founder population of primordial germ cells that are set aside early in embryogenesis. Primordial germ cells arise from the proximal epiblast, a region of the early mouse embryo that also contributes to the first blood lineages of the embryonic yolk sac. Embryonic stem cells differentiate in
Niels, Geijsen, Melissa, Horoschak, Kitai, Kim, Joost, Gribnau, Kevin, Eggan, George Q, Daley   +5 more
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Derivation of Germ Cells from Embryonic Stem Cells

2006
Embryonic stem cells (ESCs), derivatives of cells of early mammalian embryos, have proven to be one of the most powerful tools in developmental and stem cell biology. When injected into embryos, ESCs can contribute to tissues derived from all three germ layers and to the germline.
J, Kehler, K, Hübner, H R, Schöler
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Comparative transcript profiles of cell cycle-related genes in mouse primordial germ cells, embryonic stem cells and embryonic germ cells

Gene Expression Patterns, 2007
We used cDNA array to compare the relative transcript levels of 96 cell cycle-related genes in mouse primordial germ cells (PGCs), embryonic germ (EG) cells and embryonic stem (ES) cells. Among 38 genes of the G1 phase analysed, Ccnd3 (CyclinD3), Cdkn1c (p57(kip2)), Rb1, and Tceb1l (Skip1-like) were expressed at significantly higher levels in PGCs than
Sorrentino, E, NAZZICONE, VALERIA, FARINI, DONATELLA, CAMPAGNOLO, LUISA, DE FELICI, MASSIMO   +4 more
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Isolation of Murine Embryonic Stem and Embryonic Germ Cells by Selective Ablation

Transgenic Research, 2003
The isolation of murine embryonic stem (ES) cells has been almost exclusively from the 129 mouse strain. Other mouse strains, such as CBA, have proven refractory to ES cell isolation by conventional means. We previously reported the isolation of 87.5% CBA ES lines by selective ablation of differentiating cells (McWhir et al., 1996). Here, we report the
Ed J, Gallagher, Peter, Lodge, Ray, Ansell, Jim, McWhir   +3 more
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[Embryonal germ cells and germ cell tumors].

Verhandlungen der Deutschen Gesellschaft fur Pathologie, 2008
Testicular germ cell tumors comprise of group of pluripotent tumors including seminomas and nonseminomas, arise from intratubular germ cell neoplasia and originate from the primordial germ cells/ gonocytes. Many well characterized markers of embryonic stem cells including CD9, PODXL and centromere-specific histone-H3-like protein CENPA are consistently
K, Biermann, L C, Heukamp, D, Nettersheim, K, Steger, H, Zhou, F E, Franke, I, Guetgemann, V, Sonnack, R, Brehm, J, Berg, P J, Bastian, S C, Müller, L, Wang-Eckert, H, Schorle, R, Büttner   +14 more
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Hematopoietic Development of Primordial Germ Cell-Derived Mouse Embryonic Germ Cells in Culture

Biochemical and Biophysical Research Communications, 1999
Induction of hematopoiesis in an embryonic germ (EG) cell line derived from mouse primordial germ cells (PGCs) was examined. When single undifferentiated EG-1 cells were inoculated directly into the methylcellulose medium, both primitive and definitive erythropoiesis were seen in embryoid bodies derived from the EG cells as observed in ES cells, and ...
T, Ohtaka, Y, Matsui, M, Obinata
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Optimization of porcine embryonic germ cell culture system

In Vitro Cellular & Developmental Biology - Animal, 2020
Homologous feeder culture system can efficiently promote the proliferation of embryonic germ (EG) cells or embryonic stem (ES) cells while avoiding contamination by exogenous proteins and pathogens. In this study, we compared the potency of using homologous porcine embryonic fibroblasts (PEFs), gonadal stromal cells (GSCs), porcine adipose-derived stem
Yang, Li, Yu, Qiao, Fei, Li, Hongjun, Wang, Xiao, Dong   +4 more
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Characteristics of human embryonic stem cells, embryonal carcinoma cells and embryonic germ cells

2004
A stem cell can replicate itself and produce cells that take on more specialized functions. The breadth of function adopted by the more differentiated daughter cells and their progeny is commonly referred to as the developmental potential or potency of the stem cell. The inner cell mass gives rise to two groups of cells.
Michael J. Shamblott, Jared L. Sterneckert   +1 more
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Immunomagnetic Isolation of Primordial Germ Cells and the Establishment of Embryonic Germ Cell Lines in the Mouse

Cloning, 1999
The stage-specific embryonic antigen 1 (SSEA-1) is a cell marker of primordial germ cells (PGCs). In the present study, it is shown that isolation and purification of PGCs from 8.5-11.5 days post coitum (dpc) embryos can be achieved by a immunomagnetic cell sorting method using SSEA-1 antibody-conjugated magnetic beads, and then the sorted PGCs can be ...
G, Durcova-Hills, T, Tokunaga, S, Kurosaka, M, Yamaguchi, S, Takahashi, H, Imai   +5 more
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Modeling Human Germ Cell Development with Embryonic Stem Cells

Regenerative Medicine, 2005
There has previously been no robust cell-based model for examining the genetic and epigenetic mechanisms of human germ cell formation. Human embryonic stem cells (hESCs) could potentially fill this need, as all cell types analyzed to date (including mature germ cells) can be identified by marker analysis during hESC differentiation.
Amander T, Clark, Renee A, Reijo Pera
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