Hydrogel-assisted functional reconstitution of human P-glycoprotein (ABCB1) in giant liposomes. [PDF]
Horger KS +6 more
europepmc +2 more sources
Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. [PDF]
After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
Carlos E S Guedes +4 more
doaj +1 more source
Electrochemiluminescence Imaging of Liposome Permeabilization by an Antimicrobial Peptide: Melittin
The permeabilization of liposomes by melittin, an antimicrobial peptide (AMP), has been studied by an electrochemiluminescence (ECL) imaging strategy. The methodology consisted first of encapsulating ECL reagents in sealed giant asymmetrical liposomes ...
Fatma Ben Trad +8 more
doaj +1 more source
“Force-From-Lipids” Dependence of the MscCG Mechanosensitive Channel Gating on Anionic Membranes
Mechanosensory transduction in Corynebacterium glutamicum plays a major role in glutamate efflux for industrial MSG, whose production depends on the activation of MscCG-type mechanosensitive channels.
Yoshitaka Nakayama +2 more
doaj +1 more source
Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock [PDF]
Giant unilamellar vesicles (GUVs), composed of a phospholipid bilayer, are often used as a model system for cell membranes. However, the study of proteo-membrane interactions in this system is limited as the incorporation of integral and lipid-anchored proteins into GUVs remains challenging.
Motta, Isabelle +6 more
openaire +3 more sources
Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes [PDF]
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the ...
A Fürstner +41 more
core +1 more source
Disruptions and perturbations of the cellular plasma membrane by peptides have garnered significant interest in the elucidation of biological phenomena.
Mathew Sebastiao +3 more
doaj +1 more source
Formation of Oligovesicular Vesicles by Micromanipulation
Cell-sized lipid bilayer membrane vesicles (giant vesicles, GVs) or semi-vesicles were formed from egg yolk phosphatidylcholine on a platinum electrode under applied electric voltage by electroformation.
Yukihisa Okumura +2 more
doaj +1 more source
Single-molecule analysis of the entire perfringolysin O pore formation pathway
The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of
Conall McGuinness +7 more
doaj +1 more source
Protrusive growth from giant liposomes driven by actin polymerization [PDF]
Development of protrusions in the cell is indispensable in the process of cell motility. Membrane protrusion has long been suggested to occur as a result of actin polymerization immediately beneath the cell membrane at the leading edge, but elucidation of the mechanism is insufficient because of the complexity of the cell.
H, Miyata +3 more
openaire +2 more sources

