Results 21 to 30 of about 431,797 (248)

Hydrogel-assisted functional reconstitution of human P-glycoprotein (ABCB1) in giant liposomes. [PDF]

open access: yesBiochim Biophys Acta, 2015
Horger KS   +6 more
europepmc   +2 more sources

Encapsulation of Living Leishmania Promastigotes in Artificial Lipid Vacuoles. [PDF]

open access: yesPLoS ONE, 2015
After phagocytosis by mammalian macrophages, promastigote forms of Leishmania parasites settle inside intracellular parasitophorous vacuoles (PVs) in which they transform into amastigote forms and replicate.
Carlos E S Guedes   +4 more
doaj   +1 more source

Electrochemiluminescence Imaging of Liposome Permeabilization by an Antimicrobial Peptide: Melittin

open access: yesChemical & Biomedical Imaging, 2023
The permeabilization of liposomes by melittin, an antimicrobial peptide (AMP), has been studied by an electrochemiluminescence (ECL) imaging strategy. The methodology consisted first of encapsulating ECL reagents in sealed giant asymmetrical liposomes ...
Fatma Ben Trad   +8 more
doaj   +1 more source

“Force-From-Lipids” Dependence of the MscCG Mechanosensitive Channel Gating on Anionic Membranes

open access: yesMicroorganisms, 2023
Mechanosensory transduction in Corynebacterium glutamicum plays a major role in glutamate efflux for industrial MSG, whose production depends on the activation of MscCG-type mechanosensitive channels.
Yoshitaka Nakayama   +2 more
doaj   +1 more source

Formation of Giant Unilamellar Proteo-Liposomes by Osmotic Shock [PDF]

open access: yesLangmuir, 2015
Giant unilamellar vesicles (GUVs), composed of a phospholipid bilayer, are often used as a model system for cell membranes. However, the study of proteo-membrane interactions in this system is limited as the incorporation of integral and lipid-anchored proteins into GUVs remains challenging.
Motta, Isabelle   +6 more
openaire   +3 more sources

Using small molecules to facilitate exchange of bicarbonate and chloride anions across liposomal membranes [PDF]

open access: yes, 2009
Bicarbonate is involved in a wide range of biological processes, which include respiration, regulation of intracellular pH and fertilization. In this study we use a combination of NMR spectroscopy and ion-selective electrode techniques to show that the ...
A Fürstner   +41 more
core   +1 more source

Fluorescence Resonance Energy Transfer to Detect Plasma Membrane Perturbations in Giant Plasma Membrane Vesicles

open access: yesBio-Protocol, 2023
Disruptions and perturbations of the cellular plasma membrane by peptides have garnered significant interest in the elucidation of biological phenomena.
Mathew Sebastiao   +3 more
doaj   +1 more source

Formation of Oligovesicular Vesicles by Micromanipulation

open access: yesMembranes, 2011
Cell-sized lipid bilayer membrane vesicles (giant vesicles, GVs) or semi-vesicles were formed from egg yolk phosphatidylcholine on a platinum electrode under applied electric voltage by electroformation.
Yukihisa Okumura   +2 more
doaj   +1 more source

Single-molecule analysis of the entire perfringolysin O pore formation pathway

open access: yeseLife, 2022
The cholesterol-dependent cytolysin perfringolysin O (PFO) is secreted by Clostridium perfringens as a bacterial virulence factor able to form giant ring-shaped pores that perforate and ultimately lyse mammalian cell membranes. To resolve the kinetics of
Conall McGuinness   +7 more
doaj   +1 more source

Protrusive growth from giant liposomes driven by actin polymerization [PDF]

open access: yesProceedings of the National Academy of Sciences, 1999
Development of protrusions in the cell is indispensable in the process of cell motility. Membrane protrusion has long been suggested to occur as a result of actin polymerization immediately beneath the cell membrane at the leading edge, but elucidation of the mechanism is insufficient because of the complexity of the cell.
H, Miyata   +3 more
openaire   +2 more sources

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