Results 201 to 210 of about 22,955 (239)

The expression of glia maturation factors and the effect of glia maturation factor-γ on angiogenic sprouting in zebrafish

Experimental Cell Research, 2013
Angiogenesis is a vital process for proper embryonic development, wound healing, malignant tumor growth and metastasis. Two glia maturation factor genes, glia maturation factor-β (GMFB) and glia maturation factor-γ (GMFG), presenting different expression patterns and distinct biological functions are found in vertebrates. But, the role of GMFB and GMFG
Peng, Zuo, Zhiqin, Fu, Ting, Tao, Feng, Ye, Lili, Chen, Xinyu, Wang, Weiguo, Lu, Xing, Xie   +7 more
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An improved procedure for the isolation of glia maturation factor

Journal of Cellular Physiology, 1984
AbstractA procedure for the bulk isolation of glia maturation factor (GMF) in high yield and high purity from bovine brains is outlined. The method involves extraction by homogenization and centrifugation, followed by ammonium sulfate precipitation and column chromatography with diethylaminoethyl (DEAE) Sephacel, Sephadex G‐75, and hydroxylapatite. The
R, Lim, J F, Miller
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Disulfide isoforms of recombinant glia maturation factor β

Biochemical and Biophysical Research Communications, 1990
Recombinant human glia maturation factor beta (r-hGMF-beta) is a single-chain polypeptide (141 amino acid residues) containing three cysteines, at positions 7, 86 and 95. Nascent r-hGMF-beta exists in the reduced state and has no biological activity. The protein can be activated through oxidative refolding by incubation with a mixture of reduced and ...
A, Zaheer, R, Lim
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Interaction of Glia maturation factor with the glial cell membrane

Brain Research, 1982
Glia maturation factor (GMF) immobilized on agarose beads retained the same mitogenic and morphological transforming activities as free GMF when tested on glioblasts. The exposure of glioblasts to immobilized GMF for 5 min provoked the initiation of DNA synthesis and maximal stimulation was obtained within 30 min.
J, Ito, T, Kato, Y, Yamakawa, H, Kato, Y, Sakazaki, R, Lim, R, Tanaka   +6 more
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Structure and Function of Glia Maturation Factor Beta

1991
In 1972 this laboratory observed the ability of brain extracts to promote the phenotypic expression of cultured astrocytes and named the active agent “glia maturation factor” (GMF) (Lim et al., 1972, 1973; Lim and Mitsunobu, 1974). Attempts at purifying this factor encountered a number of difficulties. First, it exists in low abundance.
R, Lim, A, Zaheer
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Induction of cytoskeletal alterations in C6 glioma by glia maturation factor

International Journal of Developmental Neuroscience, 1987
AbstractC6 rat glioma cells respond to glia maturation factor (GMF) with characteristic morphological alterations. Observed under phase‐contrast microscopy, the cells changed from a rounded morphology in random formation to a spindle‐shaped appearance in parallel arrays.
T C, Ryken, J W, Turner, D J, Hicklin, J F, Miller, B A, Baggenstoss, R, Lim   +5 more
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Enucleation Blocks the Morphological Response of Glioblasts to Glia Maturation Factor

Developmental Neuroscience, 2007
Culture glioblasts obtained from rat fetuses were enucleated with cytochalasin B. The glia maturation factor stimulated the morphological differentiation of nucleated but not enucleated cells. In contrast, 8-bromo-cyclic AMP stimulated the morphological differentiation of both nucleated and enucleated cells. This distinction implies a difference in the
R, Lim, S S, Troy, D E, Turriff, J V, Prunskis   +3 more
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Glia Maturation Factor: Effect on Chemical Differentiation of Glioblasts in Culture

Science, 1977
A protein factor from the adult brain increases the concentrations of adenosine 3′,5′-monophosphate and S-100 protein in glioblasts in culture. Such changes are correlated with the outgrowth of cell processes.
R, Lim, D E, Turriff, S S, Troy, B W, Moore, L F, Eng   +4 more
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Purification of bovine glia maturation factor and characterization with monoclonal antibody

Biochemistry, 1985
Glia maturation factor (GMF) is purified 100 000-fold to apparent homogeneity from bovine brains by a procedure consisting of ammonium sulfate precipitation, column chromatography with diethylaminoethyl-Sephacel, Sephadex G-75, and hydroxylapatite, and a final step using C4 reverse-phase high-performance liquid chromatography.
R, Lim, J F, Miller, D J, Hicklin, A A, Andresen   +3 more
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Temperature Dependence of Morphological Response of Glioblasts to Glia Maturation Factor

Developmental Neuroscience, 2007
Glioblasts respond to glia maturation factor (GMF) by outgrowth of cell processes. This morphological response takes place at 37 °C but not at 23 °C, suggesting the involvement of temperature-dependent chemical processes in the mechanism of action of GMF.
D E, Turriff, R, Lim
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