Results 11 to 20 of about 41,955 (229)
Fluorescence-based microtiter plate assay for glutamate–cysteine ligase activity [PDF]
Analytical Biochemistry, 2003 Glutamate-cysteine ligase (GCL; also known as gamma-glutamylcysteine synthetase) is the rate-limiting enzyme in glutathione (GSH) synthesis. Traditional assays for the activity of this enzyme are based either on coupled reactions with other enzymes or on high-performance liquid chromatography (HPLC) assessment of gamma-glutamylcysteine (gamma-GC ...
Collin C, White +4 more
openaire +4 more sources
Rapid Activation of Glutamate Cysteine Ligase following Oxidative Stress [PDF]
Journal of Biological Chemistry, 2010 Glutamate cysteine ligase (GCL) catalyzes the rate-limiting step in the formation of the cellular antioxidant glutathione (GSH). The GCL holoenzyme consists of two separately coded proteins, a catalytic subunit (GCLC) and a modifier subunit (GCLM). Both GCLC and GLCM are controlled transcriptionally by a variety of cellular stimuli, including oxidative
Cecile M, Krejsa +5 more
openaire +4 more sources
Expression and Characterization of Human Glutamate–Cysteine Ligase
Archives of Biochemistry and Biophysics, 1998 Glutamate-cysteine ligase (GLCL) catalyzes the rate-limiting step in glutathione biosynthesis. GLCL comprises regulatory (GLCLR) and catalytic (GLCLC) subunits. To understand better the structure-function relationship of GLCL subunits and holoenzyme, human GLCLR and GLCLC genes were inserted into the baculovirus genome.
Z, Tu, M W, Anders
openaire +4 more sources
Knockdown of glutamate cysteine ligase catalytic subunit by siRNA causes the gold nanoparticles-induced cytotoxicity in lung cancer cells. [PDF]
PLoS ONE, 2015 Gold nanoparticles (GNPs) have shown promising medical applications in cancer treatment involved in the regulation of intracellular redox balance. Previously, we have reported that GNPs can trigger apoptosis and necrosis in human lung cancer cells (A549)
Min Liu, Yunxue Zhao, Xiumei Zhang
doaj +2 more sources
Optimization and application of glutamate cysteine ligase measurement in wildlife species
Ecotoxicology and Environmental Safety, 2009 Glutamate cysteine ligase (GCL), synthesizing gamma-glutamylcysteine from glutamate and cysteine, is the rate-limiting enzyme in glutathione (GSH) biosynthesis. GCL activity measurement was optimized in tissues from deer mice, Sprague Dawley rats, and mallard ducks.
Hongmei, Wu +5 more
openaire +4 more sources
Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. [PDF]
J Biol Chem, 2012 GSH is synthesized sequentially by glutamate-cysteine ligase (GCL) and GSH synthase and defends against oxidative stress, which promotes hepatic stellate cell (HSC) activation. Changes in GSH synthesis during HSC activation are poorly characterized.
Ramani K +4 more
europepmc +4 more sources
Post-translational Activation of Glutamate Cysteine Ligase with Dimercaprol: A NOVEL MECHANISM OF INHIBITING NEUROINFLAMMATION IN VITRO. [PDF]
J Biol Chem, 2017 McElroy PB, Sri Hari A, Day BJ, Patel M.
europepmc +3 more sources
Journal of Food Quality, 2022 This study aims to evaluate the protective effects of feruloyl oligosaccharides from fermented wheat bran (FOs-FWB) against oxidative stress in IPEC-J2 cells in vitro and in a zebrafish model in vivo.
Jia Zhang +8 more
doaj +1 more source
Nature Communications, 2022 Follicular and marginal zone B (FoB and MZB, respectively) cells have divergent metabolic characteristics. Here the authors show that deficiency of glutamate cysteine ligase (Gclc), the enzyme for glutathione synthesis, differentially impacts FoB and MZB
Davide G. Franchina +21 more
doaj +1 more source
Nrf2 Regulation by Curcumin: Molecular Aspects for Therapeutic Prospects
Molecules, 2021 Nuclear factor erythroid 2 p45-related factor (2Nrf2) is an essential leucine zipper protein (bZIP) that is primarily located in the cytoplasm under physiological conditions.
Seyed Hossein Shahcheraghi +6 more
doaj +1 more source