Membrane glycomics reveal heterogeneity and quantitative distribution of cell surface sialylation. [PDF]
, 2018 Given that unnatural sugar expression is metabolically achieved, the kinetics and disposition of incorporation can lend insight into the temporal and localization preferences of sialylation across the cell surface.
Haigh, Nathan E +9 more
core
The GalNAc-type O-Glycoproteome of CHO Cells Characterized by the SimpleCell Strategy [PDF]
, 2014 The Chinese hamster ovary cell (CHO) is the major host cell factory for recombinant production of biological therapeutics primarily because of its “human-like” glycosylation features.
Alder Schulz, Morten +12 more
core +1 more source
Advancements in Mass Spectrometry‐Based Glycomics in Food and Nutritional Science
Mass Spectrometry Reviews, EarlyView.ABSTRACT This review highlights advancements in mass spectrometry (MS)‐based glycomics in food and nutritional science. Carbohydrates, which are vital for human health, exhibit complex structures, making their analysis challenging. MS has become an indispensable tool for elucidating the structures of carbohydrates, including glycans, through soft ...
JaeHui Song +3 more
wiley +1 more source
Synthetic glycopeptides and glycoproteins with applications in biological research
Beilstein Journal of Organic Chemistry, 2012 Over the past few years, synthetic methods for the preparation of complex glycopeptides have been drastically improved. The need for homogenous glycopeptides and glycoproteins with defined chemical structures to study diverse biological phenomena further
Ulrika Westerlind
doaj +1 more source
Identification of Glycopeptides with Multiple Hydroxylysine O-Glycosylation Sites by Tandem Mass Spectrometry [PDF]
, 2015 Glycosylation is one of the most common post-translational modifications in proteins, existing in ∼50% of mammalian proteins. Several research groups have demonstrated that mass spectrometry is an efficient technique for glycopeptide identification ...
Li, Shuai Cheng +6 more
core +2 more sources
Data‐Independent Acquisition Mass Spectrometry in Tumor Classification and Cancer Biomarker Research
Mass Spectrometry Reviews, EarlyView.Abstract Cancer treatment is far from optimal also because current classification systems do not reflect the complex molecular status of the tumor and its phenotype in sufficient detail. To construct molecular tumor classifiers, omics tools provide complex molecular data reflecting many aspects from genotype to phenotype.
Jan Simonik +3 more
wiley +1 more source
Molecules, 2010 Three 18-residue peptides with the sequence Glp-Asp-Thr-Thr-Asp-Glu-Trp-Asp-Arg-Asp-Leu-Glu-Asn-Leu-Ser-Thr-Thr-Lys, taken from the N-terminus of the rat epididymal cysteine-rich secretory protein (Crisp-1) that is important in the fertilization process,
George Barany +2 more
doaj +1 more source
How to Dig Deeper? Improved Enrichment Methods for Mucin Core-1 Type Glycopeptides [PDF]
, 2012 Two different workflows were tested in order to develop methods that provide deeper insight into the secreted O-glycoproteome. Bovine serum samples were subjected to lectin affinity-chromatography both at the protein- and peptide-level in order to ...
Darula, Zsuzsanna +2 more
core +1 more source
Advancing Extracellular Vesicle Research: A Review of Systems Biology and Multiomics Perspectives
PROTEOMICS, EarlyView.ABSTRACT Extracellular vesicles (EVs) are membrane‐bound vesicles secreted by various cell types into the extracellular space and play a role in intercellular communication. Their molecular cargo varies depending on the cell of origin and its functional state.
Gloria Kemunto +2 more
wiley +1 more source
Climate change and temperature dependent biogeography: oxygen limitation of thermal tolerance in animals [PDF]
, 2001 Recent years have shown a rise in mean global temperatures and a shift in the geographical distribution of ectothermic animals. For a cause and effect analysis the present paper discusses those physiological processes limiting thermal tolerance.
Pörtner, Hans-Otto
core +1 more source