Results 11 to 20 of about 4,333 (173)

Fibrin and D-dimer bind to monomeric GPVI [PDF]

Blood Advances, 2017
: Fibrin has recently been shown to activate platelets through the immunoglobulin receptor glycoprotein VI (GPVI). In the present study, we show that spreading of human platelets on fibrin is abolished in patients deficient in GPVI, confirming that ...
Marie-Blanche Onselaer, Alexander T. Hardy, Clare Wilson, Ximena Sanchez, Amir K. Babar, Jeanette L.C. Miller, Callum N. Watson, Stephanie K. Watson, Arkadiusz Bonna, Helen Philippou, Andrew B. Herr, Diego Mezzano, Robert A.S. Ariëns, Steve P. Watson   +13 more
doaj   +6 more sources

Phytochemical Profiling, Antiplatelet Potential, and Cytotoxic Assessment of Thymelaea tartonraira Aerial Part Essential Oil: In Vitro and In Silico Studies. [PDF]

Chem Biodivers
Essential oil from Thymelaea tartonraira was characterized by GC–MS and evaluated using in vitro and in silico approaches. The oil exhibited notable antiplatelet activity with low cytotoxicity. Molecular docking revealed strong interactions of major compounds with GPVI, COX‐1, and P2Y12, supporting its potential as a natural antiplatelet agent ...
Soltani S, Haffouz A, Koubaa I, Gargouri A, Jerbi A, Amor IB, Gargouri J, HadjKacem B, Allouche N.   +8 more
europepmc   +2 more sources

Characterization of GPVI- or GPVI-CD39-Coated Nanoparticles and Their Impact on In Vitro Thrombus Formation [PDF]

International Journal of Molecular Sciences, 2021
Traditional antithrombotic agents commonly share a therapy-limiting side effect, as they increase the overall systemic bleeding risk. A novel approach for targeted antithrombotic therapy is nanoparticles. In other therapeutic fields, nanoparticles have enabled site-specific delivery with low levels of toxicity and side effects.
Jeremy A. Nestele, Anne-Katrin Rohlfing, Valerie Dicenta, Alexander Bild, Daniela Eißler, Frederic Emschermann, Marcel Kremser, Konstantin Krutzke, Tilman E. Schäffer, Oliver Borst, Moran Levi, Netanel Korin, Meinrad Paul Gawaz   +12 more
openaire   +3 more sources

Platelet Rubicon Bidirectional Regulation of GPVI and Integrin αIIbβ3 Signaling Mitigates Stroke Infarction Without Compromising Hemostasis. [PDF]

Adv Sci (Weinh)
This study identifies Rubicon as a key platelet protein that bidirectionally regulates GPVI and integrin αIIbβ3 signaling. Platelet Rubicon protects against cerebral ischemia‐reperfusion injury by limiting infarction without increasing hemorrhage.
Chen X, Li J, Liu Y, Li L, Deng X, Sheng Y, Zhu X, Jiang X, Li W, Cai X, Sun Q, Hu H.   +11 more
europepmc   +2 more sources

mTOR regulates GPVI-mediated platelet activation [PDF]

Journal of Translational Medicine, 2021
Abstract Background Due to mTOR (mammalian/mechanistic target of rapamycin) gene-loss mice die during embryonic development, the role of mTOR in platelets has not been evaluated using gene knockout technology. Methods A mouse model with megakaryocyte/platelet-specific ...
Longsheng Wang, Gang Liu, Nannan Wu, Baiyun Dai, Shuang Han, Qiaoyun Liu, Fang Huang, Zhihua Chen, Weihong Xu, Dajing Xia, Cunji Gao   +10 more
openaire   +3 more sources

Structural characterization of a novel GPVI-nanobody complex reveals a biologically active domain-swapped GPVI dimer [PDF]

Blood, 2021
Abstract Glycoprotein VI (GPVI) is the major signaling receptor for collagen on platelets. We have raised 54 nanobodies (Nb), grouped into 33 structural classes based on their complementary determining region 3 loops, against recombinant GPVI-Fc (dimeric GPVI) and have characterized their ability to bind recombinant GPVI, resting and ...
Alexandre Slater, Ying Di, Joanne C. Clark, Natalie J. Jooss, Eleyna M. Martin, Fawaz Alenazy, Mark R. Thomas, Robert A. S. Ariëns, Andrew B. Herr, Natalie S. Poulter, Jonas Emsley, Steve P. Watson   +11 more
openaire   +4 more sources

Evidence that GPVI is Expressed as a Mixture of Monomers and Dimers, and that the D2 Domain is not Essential for GPVI Activation

Thrombosis and Haemostasis, 2021
AbstractCollagen has been proposed to bind to a unique epitope in dimeric glycoprotein VI (GPVI) and the number of GPVI dimers has been reported to increase upon platelet activation. However, in contrast, the crystal structure of GPVI in complex with collagen-related peptide (CRP) showed binding distinct from the site of dimerization.
Joanne C. Clark, Raluca A. I. Neagoe, Malou Zuidscherwoude, Deirdre M. Kavanagh, Alexandre Slater, Eleyna M. Martin, Mark Soave, David Stegner, Bernhard Nieswandt, Natalie S. Poulter, Johan Hummert, Dirk-Peter Herten, Michael G. Tomlinson, Stephen J. Hill, Steve P. Watson   +14 more
openaire   +3 more sources

Soluble GPVI is elevated in injured patients: shedding is mediated by fibrin activation of GPVI [PDF]

Blood Advances, 2018
Key Points Soluble GPVI is elevated in patients with thermal injury with sepsis, and sGPVI levels augment severity score prediction of mortality.
Samantha J. Montague, Céline Delierneux, Christelle Lecut, Nathalie Layios, Robert J. Dinsdale, Christine S.-M. Lee, Natalie S. Poulter, Robert K. Andrews, Peter Hampson, Christopher M. Wearn, Nathalie Maes, Jonathan Bishop, Amy Bamford, Chris Gardiner, Woei Ming Lee, Tariq Iqbal, Naiem Moiemen, Steve P. Watson, Cécile Oury, Paul Harrison, Elizabeth E. Gardiner   +20 more
openaire   +6 more sources

Immobilized collagen prevents shedding and induces sustained GPVI clustering and signaling in platelets

Platelets, 2021
Collagen, the most thrombogenic constituent of blood vessel walls, activates platelets through glycoprotein VI (GPVI). In suspension, following platelet activation by collagen, GPVI is cleaved by A Disintegrin And Metalloproteinase (ADAM)10 and ADAM17 ...
Chiara Pallini, Jeremy A. Pike, Christopher O’Shea, Robert K. Andrews, Elizabeth E. Gardiner, Steve P. Watson, Natalie S. Poulter   +6 more
doaj   +1 more source

Absence of bleeding upon dual antiplatelet therapy in a patient with a immune GPVI deficiency

Platelets, 2021
Acquired deficiencies in platelet glycoprotein VI are rare and have not been found associated with other defects. Here we report the case of a 64-year old male patient presenting an immune GPVI deficiency associated to a mutation in the alpha-actinin ...
Stéphane Loyau Inserm, Dorothée Faille, Philippe Gautier, Paquita Nurden, Martine Jandrot-Perrus, Nadine Ajzenberg   +5 more
doaj   +1 more source