Results 11 to 20 of about 6,222 (193)

Fibrin and D-dimer bind to monomeric GPVI [PDF]

Blood Advances, 2017
: Fibrin has recently been shown to activate platelets through the immunoglobulin receptor glycoprotein VI (GPVI). In the present study, we show that spreading of human platelets on fibrin is abolished in patients deficient in GPVI, confirming that ...
Marie-Blanche Onselaer, Alexander T. Hardy, Clare Wilson, Ximena Sanchez, Amir K. Babar, Jeanette L.C. Miller, Callum N. Watson, Stephanie K. Watson, Arkadiusz Bonna, Helen Philippou, Andrew B. Herr, Diego Mezzano, Robert A.S. Ariëns, Steve P. Watson   +13 more
doaj   +8 more sources

Characterization of GPVI- or GPVI-CD39-Coated Nanoparticles and Their Impact on In Vitro Thrombus Formation [PDF]

International Journal of Molecular Sciences, 2021
Traditional antithrombotic agents commonly share a therapy-limiting side effect, as they increase the overall systemic bleeding risk. A novel approach for targeted antithrombotic therapy is nanoparticles. In other therapeutic fields, nanoparticles have enabled site-specific delivery with low levels of toxicity and side effects.
Jeremy A. Nestele, Anne-Katrin Rohlfing, Valerie Dicenta, Alexander Bild, Daniela Eißler, Frederic Emschermann, Marcel Kremser, Konstantin Krutzke, Tilman E. Schäffer, Oliver Borst, Moran Levi, Netanel Korin, Meinrad Paul Gawaz   +12 more
openaire   +4 more sources

Structural characterization of a novel GPVI-nanobody complex reveals a biologically active domain-swapped GPVI dimer [PDF]

Blood, 2021
Abstract Glycoprotein VI (GPVI) is the major signaling receptor for collagen on platelets. We have raised 54 nanobodies (Nb), grouped into 33 structural classes based on their complementary determining region 3 loops, against recombinant GPVI-Fc (dimeric GPVI) and have characterized their ability to bind recombinant GPVI, resting and ...
Alexandre Slater, Ying Di, Joanne C. Clark, Natalie J. Jooss, Eleyna M. Martin, Fawaz Alenazy, Mark R. Thomas, Robert A. S. Ariëns, Andrew B. Herr, Natalie S. Poulter, Jonas Emsley, Steve P. Watson   +11 more
openaire   +6 more sources

Anti‐GPVI nanobody blocks collagen‐ and atherosclerotic plaque–induced GPVI clustering, signaling, and thrombus formation [PDF]

Journal of Thrombosis and Haemostasis, 2022
The collagen receptor glycoprotein VI (GPVI) is an attractive antiplatelet target due to its critical role in thrombosis but minor involvement in hemostasis.To investigate GPVI receptor involvement in platelet activation by collagen-I and atherosclerotic plaque using novel blocking and non-blocking anti-GPVI nanobodies (Nbs).Nb effects on GPVI-mediated
Natalie J. Jooss, Christopher W. Smith, Alexandre Slater, Samantha J. Montague, Ying Di, Christopher O'Shea, Mark R. Thomas, Yvonne M.C. Henskens, Johan W.M. Heemskerk, Steve P. Watson, Natalie S. Poulter   +10 more
openaire   +3 more sources

Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets [PDF]

Journal of Thrombosis and Haemostasis, 2017
Essentials - Dimeric high-affinity collagen receptor glycoprotein VI (GPVI) is present on resting platelets. - Spatio-temporal organization of platelet GPVI-dimers was evaluated using advanced microscopy. - Upon platelet adhesion to collagenous substrates, GPVI-dimers coalesce to form clusters.
Poulter, N. S., Pollitt, A. Y., Owen, D. M., Gardiner, E. E., Andrews, R. K., Shimizu, H., Ishikawa, D., Bihan, D., Farndale, R. W., Moroi, M., Watson, S. P., Jung, S. M.   +11 more
openaire   +7 more sources

Rac Inhibition Causes Impaired GPVI Signalling in Human Platelets through GPVI Shedding and Reduction in PLCγ2 Phosphorylation [PDF]

International Journal of Molecular Sciences, 2022
Rac1 is a small Rho GTPase that is activated in platelets upon stimulation with various ligands, including collagen and thrombin, which are ligands for the glycoprotein VI (GPVI) receptor and the protease-activated receptors, respectively. Rac1-deficient murine platelets have impaired lamellipodia formation, aggregation, and reduced PLCγ2 activation ...
Raluca A. I. Neagoe, Elizabeth E. Gardiner, David Stegner, Bernhard Nieswandt, Steve P. Watson, Natalie S. Poulter   +5 more
openaire   +5 more sources

Affimer reagents as tool molecules to modulate platelet GPVI-ligand interactions and specifically bind GPVI dimer [PDF]

Blood Advances
Abstract Glycoprotein VI (GPVI) plays a key role in collagen-induced platelet aggregation. Affimers are engineered binding protein alternatives to antibodies. We screened and characterized GPVI-binding Affimers as novel tools to probe GPVI function.
Xu, R-G, Tiede, C, Calabrese, AN, Cheah, LT, Adams, TL, Sandrin Gauer, JS, Hindle, MS, Webb, BA, Yates, DM, Slater, A, Duval, C, Naseem, KM, Herr, AB, Tomlinson, DC, Watson, SP, Ariëns, RAS   +15 more
core   +7 more sources

Fibrin activates GPVI in human and mouse platelets [PDF]

Blood, 2015
Key PointsFibrin is a novel ligand for the platelet collagen receptor, GPVI, increasing platelet procoagulant activity. Activation of GPVI by fibrin contributes to thrombus growth and stabilization.
Osama M, Alshehri, Craig E, Hughes, Samantha, Montague, Stephanie K, Watson, Jon, Frampton, Markus, Bender, Steve P, Watson   +6 more
openaire   +3 more sources

Liver-mediated shedding of platelet GPVI

Blood, 2016
The in vivo mechanisms underlying antibody-based shedding of the key platelet collagen receptor glycoprotein VI (GPVI) are potentially significant for future antibody-based thromboprotective agents.
Berndt, Michael, Andrews, R.
core   +5 more sources

Differential Inhibition of Human Atherosclerotic Plaque–Induced Platelet Activation by Dimeric GPVI-Fc and Anti-GPVI Antibodies [PDF]

Journal of the American College of Cardiology, 2015
Glycoprotein VI (GPVI) is the essential platelet collagen receptor in atherothrombosis, but its inhibition causes only a mild bleeding tendency. Thus, targeting this receptor has selective antithrombotic potential.This study sought to compare compounds interfering with platelet GPVI-atherosclerotic plaque interaction to improve current ...
Jamasbi, Janina, Megens, Remco TA, Bianchini, Mariaelvy, Münch, Götz, Ungerer, Martin, Faussner, Alexander, Sherman, Shachar, Walker, Adam, Goyal, Pankaj, Jung, Stephanie, Brandl, Richard, Weber, Christian, Lorenz, Reinhard, Farndale, Richard, Elia, Natalie, Siess, Wolfgang   +15 more
openaire   +6 more sources