Results 61 to 70 of about 6,222 (193)

Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis

Frontiers in Pharmacology, 2023
Introduction: Luteolin inhibits platelet activation and thrombus formation, but the mechanisms are unclear. This study investigated the effects of luteolin on GPVI-mediated platelet activation in vitro and explored the effect of luteolin on thrombosis ...
Yujia Ye, Lihong Yang, Min Leng, Qian Wang, Jiankui Wu, Wen Wan, Huawei Wang, Longjun Li, Yunzhu Peng, Shengjie Chai, Zhaohui Meng   +10 more
doaj   +1 more source

Defining the role of αC helix interactions in the activation of the integrin αI domain

The FEBS Journal, EarlyView.
The α2I domain of the α2β1 integrin contains a distinctive αC helix and a conserved Arg288‐Glu318 ion pair that stabilizes the closed state of the domain. Disruption of this ion pair in the activated α2I variant unwinds the αC helix, which allows interaction with additional collagen‐binding sites increasing collagen binding.
Liisa Pösö, Tomi T. Airenne, Jarmo Käpylä, Hongmin Tu, Eva Bligt‐Lindén, Vimal Parkash, Jyrki Heino, Tiina A. Salminen   +7 more
wiley   +1 more source

Testing for Non‐Severe Heritable Platelet Function Disorders

International Journal of Laboratory Hematology, EarlyView.
ABSTRACT Heritable platelet function disorders (HPFD) are a diverse group of bleeding disorders characterised by a primary qualitative defect in platelet function rather than platelet number. HPFD may be broadly categorised according to the severity of bleeding, with Glanzmann thrombasthenia and Bernard Soulier syndrome classically considered severe ...
Kate Burley, Naomi Cornish, Maria Molina Carretero, Sarah K. Westbury   +3 more
wiley   +1 more source

Downscaling to small‐volume storage bags supports platelet quality and efficient use of cold‐stored platelets for preclinical studies

Transfusion, EarlyView.
Abstract Background To maximize the utilization of cold‐stored platelets (CSPs) in preclinical research, the use of small‐volume storage bags (mini‐bags) or tubes would allow multiple treatment groups to be investigated from a single component. It is necessary to determine the effect of using downscaled storage formats on CSP quality and function ...
Janhavi Mahajan, Matthew P. Padula, Denese C. Marks, Lacey Johnson   +3 more
wiley   +1 more source

GPVI (Glycoprotein VI) Interaction With Fibrinogen Is Mediated by Avidity and the Fibrinogen αC-Region [PDF]

, 2021
Objective: GPVI (glycoprotein VI) is a key molecular player in collagen-induced platelet signaling and aggregation. Recent evidence indicates that it also plays important role in platelet aggregation and thrombus growth through interaction with fibrin ...
Stephen R. Baker, Manfield, Iain, Duval, Cedric, Baker, SR, Arkadiusz M. Bonna, Slater, Alexandre; id_orcid, Alexandre Slater, Duval, C, Rui-Gang Xu, Bonna, Arkadiusz, Eleyna M. Martin, Manfield, IW, Martin, EM, Xu, R-G, Martin, Eleyna; id_orcid, McPherson, HR, Cédric Duval, Gauer, JS, Steve P. Watson, Ariëns, Robert A. S., Bonna, AM, Helen R. McPherson, McPherson, Helen, Robert A.S. Ariëns, Gauer, Julia, Watson, S, Ariëns, RAS, Xu, Rui-Gang, Iain W. Manfield, Slater, A, Watson, Steve; id_orcid, Julia S. Gauer, Baker, Stephen   +32 more
core   +1 more source

Biochemical characterization of spleen tyrosine kinase (SYK) isoforms in platelets

Platelets, 2023
Alternate splicing is among the regulatory mechanisms imparting functional diversity in proteins. Studying protein isoforms generated through alternative splicing is therefore critical for understanding protein functions in many biological systems ...
Pankaj Kumar Singh, Carol A. Dangelmaier, Hymavathi Reddy Vari, Alexander Y. Tsygankov, Satya P. Kunapuli   +4 more
doaj   +1 more source

Continuous agitation induces aggregate formation in cold‐stored platelets during extended storage in platelet additive solution

Vox Sanguinis, EarlyView.
Abstract Background and Objectives Reduced metabolic activity of cold‐stored platelets (CSPs) has led to the assumption that agitation is not required during cold storage. However, guidelines from the Food and Drug Administration state that agitation is ‘optional’ and there is a degree of international variability described in the literature.
Janhavi Mahajan, Pearl Lei, Christopher Roan, Matthew P. Padula, Denese C. Marks, Lacey Johnson   +5 more
wiley   +1 more source

Novel antiplatelet strategies targeting GPVI, CLEC-2 and tyrosine kinases

Platelets, 2021
Antiplatelet medications comprise the cornerstone of treatment for diseases that involve arterial thrombosis, including acute coronary syndromes (ACS), stroke and peripheral arterial disease.
Maan H. Harbi, Christopher W. Smith, Phillip L. R. Nicolson, Steve P. Watson, Mark R. Thomas   +4 more
doaj   +1 more source

Protein O‐Glycosylation Shapes Lymphatic Endothelia and Lymph Node Macrophage Populations

The FASEB Journal, Volume 40, Issue 18, 30 September 2026.
To study the functional roles of O‐glycans on lymphatic endothelia, we deleted Cosmc, the chaperone required for core‐1 O‐glycan synthesis. Loss of core‐1 O‐glycans entailed major changes in glycoprotein composition of lymphatic endothelial cells (LEC), including reduced podoplanin (Pdpn), CD44, and Lyve‐1, and increased ICAM‐1; reduced CD44 was caused
Jasmin Frey, Annkathrin Ratter, Finn Brigger, Louie Cielen, Pascal Kunz, Ea Tulin, Kinga Mikos, Lothar Dieterich, Marco D'Addio, Cornelia Halin, Richard D. Cummings, Vivianne I. Otto   +11 more
wiley   +1 more source

Figure 5: SIM and STED Imaging of Collagen Stained With Not Cross-Linked GPVI-Fc (GPVI-Fc*IgG, GPVI-Fc*Fab2) and Cross-Linked GPVI-Fc (GPVI-Fc*IgG-XL, GPVI-Fc*Fab2-XL)

, 2019
GPVI-Fc was incubated with Alexa Fluor 488-conjugated anti–human-Fc IgG or anti–human-Fc Fab2 antibodies in either a 10:1 molar ratio (not cross-linked GPVI-Fc) or a 1:1 molar ratio (cross-linked GPVI-Fc).
Kerstin Uhland (2092060), Reinhard Lorenz (6820967), Remco T.A. Megens (6820955), Shachar Sherman (6820961), Natalie Elia (660866), Mariaelvy Bianchini (6820958), Christine John (839705), Götz Münch (436471), Wolfgang Siess (168198), Johannes Buchner (277160), Alexander Faussner (781036), Christian Weber (86566), Martin Ungerer (436470), Richard Brandl (6820964), Janina Jamasbi (6820952)   +14 more
core   +1 more source