Results 51 to 60 of about 1,124,962 (295)
Development and Applications of Enhanced Green Fluorescent Protein Mutants
BioTechniques, 1998 The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold–100-fold brighter fluorescence compared to wild ...
R.H. Stauber +6 more
doaj +1 more source
Green fluorescent protein as an indicator of cryoinjury in tissues. [PDF]
, 2013 The fluorescence intensity of Green Fluorescent Protein (GFP) has previously been demonstrated to be an accurate indicator of cellular viability following cryoinsult in individual GFP-transfected cells. In an attempt to ascertain whether GFP fluorescence
Aguilar, Guillermo +3 more
core +1 more source
Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum
BioTechniques, 2006 O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler +3 more
doaj +1 more source
Fluorescent Protein Approaches in Alpha Herpesvirus Research
Viruses, 2015 In the nearly two decades since the popularization of green fluorescent protein (GFP), fluorescent protein-based methodologies have revolutionized molecular and cell biology, allowing us to literally see biological processes as never before.
Ian B. Hogue +6 more
doaj +1 more source
Bioresources and Bioprocessing, 2021 Background In the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression.
Ying Li +7 more
doaj +1 more source
Fluorescent quantitation method for differentiating the nativity of green fluorescent protein [PDF]
, 2011 A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands ...
Chew, Few Ne +2 more
core +1 more source
ChemistryOpen, 2019 We report on the reflectance, transmittance and fluorescence spectra (λ=200–1200 nm) of four types of chicken eggshells (white, brown, light green, dark green) measured in situ without pretreatment and after ablation of 20–100 μm of the outer shell ...
Dr. Edwin Ostertag +4 more
doaj +1 more source
Reprogramming Hansenula polymorpha for penicillin production: expression of the Penicillium chrysogenum pcl gene [PDF]
, 2007 We aim to introduce the penicillin biosynthetic pathway into the methylotrophic yeast Hansenula polymorpha. To allow simultaneous expression of the multiple genes of the penicillin biosynthetic pathway, additional markers were required.
Gidijala, Loknath, +3 more
core +2 more sources
Green fluorescent protein as a reporter of prion protein folding
Virology Journal, 2006 Background The amino terminal half of the cellular prion protein PrPc is implicated in both the binding of copper ions and the conformational changes that lead to disease but has no defined structure. However, as some structure is likely to exist we have
Dalton Kevin +5 more
doaj +1 more source
Journal of the American Chemical Society, 2014 Genetically encoded fluorescent ribonucleic acids (RNAs) have diverse applications, including imaging RNA trafficking and as a component of RNA-based sensors that exhibit fluorescence upon binding small molecules in live cells.
Grigory S. Filonov +3 more
semanticscholar +1 more source