Results 51 to 60 of about 568,395 (338)

Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms

FEBS Letters, EarlyView.
Mycobacterium tuberculosis exhibits a remarkable resilience to acid stress. In this Review, we discuss some of the molecular mechanisms and metabolic pathways used by the tubercle bacilli to adapt and resist host‐mediated acid stress. Mycobacterium tuberculosis (Mtb) is a successful pathogen that has developed a variety of strategies to survive and ...
Janïs Laudouze, Stéphane Canaan, Alexandre Gouzy, Pierre Santucci   +3 more
wiley   +1 more source

Fluorescent proteins as tools to aid protein production

Microbial Cell Factories, 2005
Fluorescent proteins are genetically encoded, highly versatile reporters useful for monitoring various aspects of recombinant protein production. In addition to the widely popular green fluorescent protein (GFP) from Aequorea victoria, a variety of other
Su Wei
doaj   +1 more source

Green fluorescent protein [PDF]

Nature Chemistry, 2008
The 2008 Nobel Prize for Chemistry has been awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien for the discovery and development of the so-called green fluorescent ...
openaire   +2 more sources

Refining the NaV1.7 pharmacophore of a class of venom‐derived peptide inhibitors via a combination of in silico screening and rational engineering

FEBS Letters, EarlyView.
Venom peptides have shown promise in treating pain. Our study uses computer screening to identify a peptide that targets a sodium channel (NaV1.7) linked to chronic pain. We produced the peptide in the laboratory and refined its design, advancing the search for innovative pain therapies.
Gagan Sharma, Jennifer R. Deuis, Xinying Jia, Theo Crawford, Sassan Rahnama, Eivind A. B. Undheim, Irina Vetter, Yanni K. ‐Y. Chin, Mehdi Mobli   +8 more
wiley   +1 more source

Dynamics of Natural Killer cell receptor revealed by quantitative analysis of photoswitchable protein [PDF]

Biophysical Journal, Volume 105, 1-10, (2013), 2013
Natural Killer (NK) cell activation is dynamically regulated by numerous activating and inhibitory surface receptors that accumulate at the immune synapse. Quantitative analysis of receptor dynamics has been limited by methodologies which rely on indirect measurements such as fluorescence recovery after photobleaching.
arxiv   +1 more source

Time course analysis of sensory axon regeneration in vivo by directly tracing regenerating axons

Neural Regeneration Research, 2020
Most current studies quantify axon regeneration by immunostaining regeneration-associated proteins, representing indirect measurement of axon lengths from both sensory neurons in the dorsal root ganglia and motor neurons in the spinal cord.
Yan Gao, Yi-Wen Hu, Run-Shan Duan, Shu-Guang Yang, Feng-Quan Zhou, Rui-Ying Wang   +5 more
doaj   +1 more source

A Bright, Nontoxic, and Non-aggregating red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Frontiers in Cell and Developmental Biology, 2022
Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim.
Lin Ning, Yang Geng, Yang Geng, Matthew Lovett-Barron, Xiaoman Niu, Mengying Deng, Liang Wang, Niloufar Ataie, Alex Sens, Ho-Leung Ng, Shoudeng Chen, Karl Deisseroth, Michael Z. Lin, Michael Z. Lin, Jun Chu   +14 more
doaj   +1 more source

Identification of novel small molecule inhibitors of ETS transcription factors

FEBS Letters, EarlyView.
ETS transcription factors play an essential role in tumourigenesis and are indispensable for sprouting angiogenesis, a hallmark of cancer, which fuels tumour expansion and dissemination. Thus, targeting ETS transcription factor function could represent an effective, multifaceted strategy to block tumour growth. The evolutionarily conserved E‐Twenty‐Six
Shaima Abdalla, Zary Forghany, Jin Ma, Johan G. Hollander, Ruta Nachane, Karoly Szuhai, Pancras C. W. Hogendoorn, Peter ten Dijke, Dipen Shah, David A. Baker   +9 more
wiley   +1 more source

A Comparative Study of Disordered and Ordered Protein Folding Dynamics Using Computational Simulation [PDF]

arXiv, 2022
Folding protein dynamics has been an area of high interest for quite some time, especially given the increased focus on the field of Biophysics. Because folding dynamics occur on such short time scales, empirical techniques developed for more "static" protein events, such as X-ray crystallography, nuclear magnetic resonance, and green fluorescent ...
arxiv  

Development of a Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM) system using a Streak Camera [PDF]

Review of Scientific Instruments, Vol.74, 2714 (May 2003), 2003
We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations.
arxiv   +1 more source