Results 51 to 60 of about 888,780 (377)

Fluorescent Protein Approaches in Alpha Herpesvirus Research

Viruses, 2015
In the nearly two decades since the popularization of green fluorescent protein (GFP), fluorescent protein-based methodologies have revolutionized molecular and cell biology, allowing us to literally see biological processes as never before.
Ian B. Hogue, Jens B. Bosse, Esteban A. Engel, Julian Scherer, Jiun-Ruey Hu, Tony del Rio, Lynn W. Enquist   +6 more
doaj   +1 more source

Computationally designed libraries of fluorescent proteins evaluated by preservation and diversity of function [PDF]

, 2006
To determine which of seven library design algorithms best introduces new protein function without destroying it altogether, seven combinatorial libraries of green fluorescent protein variants were designed and synthesized.
Bokman, C. L. Vizcarra, Cherry, Coco, Cormack, D. Nedelcu, Dahiyat, Dwyer, Fodor, Gordon, Gordon, Heim, Hiraga, Hogrefe, Hoogenboom, Kilgard, Kuhlman, Larson, Markiewicz, Meyer, Ness, Orm , Otey, Patrick, Rennell, S. L. Mayo, Shafikhani, Socolich, Street, Street, T. P. Treynor, Voigt, Voigt   +32 more
core   +4 more sources

Development and Applications of Enhanced Green Fluorescent Protein Mutants

BioTechniques, 1998
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold–100-fold brighter fluorescence compared to wild ...
R.H. Stauber, K. Horie, P. Carney, E.A. Hudson, N.I. Tarasova, G.A. Gaitanaris, G.N. Pavlakis   +6 more
doaj   +1 more source

Pigmentation of White, Brown, and Green Chicken Eggshells Analyzed by Reflectance, Transmittance, and Fluorescence Spectroscopy

ChemistryOpen, 2019
We report on the reflectance, transmittance and fluorescence spectra (λ=200–1200 nm) of four types of chicken eggshells (white, brown, light green, dark green) measured in situ without pretreatment and after ablation of 20–100 μm of the outer shell ...
Dr. Edwin Ostertag, Miriam Scholz, Julia Klein, Prof. Dr. Karsten Rebner, Prof. Dr. Dieter Oelkrug   +4 more
doaj   +1 more source

Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum

BioTechniques, 2006
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches.
Antje Keppler, Claudio Arrivoli, Lucia Sironi, Jan Ellenberg   +3 more
doaj   +1 more source

Getting to grips with ammonium

eLife, 2013
A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.
Yi Wang, Jonathan A Javitch
doaj   +1 more source

Precise navigation surgery of tumours in the lung in mouse models enabled by in situ fluorescence labelling with a killer-reporter adenovirus. [PDF]

, 2015
BackgroundCurrent methods of image-guided surgery of tumours of the lung mostly rely on CT. A sensitive procedure of selective tumour fluorescence labelling would allow simple and high-resolution visualisation of the tumour for precise surgical ...
Bouvet, Michael, Fujiwara, Toshiyoshi, Hoffman, Robert M, Kagawa, Shunsuke, Kishimoto, Hiroyuki, Miwa, Shinji, Urata, Yasuo, Yano, Shuya, Zhang, Yong   +8 more
core   +1 more source

RNA Mimics of Green Fluorescent Protein [PDF]

Science, 2011
Selection for RNAs that bind and activate derivatives of the green fluorescent protein fluorophore yields a wide range of useful spectral properties.
Karen Y. Wu, Jeremy S. Paige, Samie R. Jaffrey   +2 more
openaire   +3 more sources

Red fluorescent proteins engineered from green fluorescent proteins

Proceedings of the National Academy of Sciences, 2023
Fluorescent proteins (FPs) form a fluorophore through autocatalysis from three consecutive amino acid residues within a polypeptide chain. The two major groups, green FPs (GFPs) and red FPs (RFPs), have distinct fluorophore structures; RFPs have an extended π-conjugation system with an additional double bond.
Hiromi Imamura, Shiho Otsubo, Mizuho Nishida, Norihiro Takekawa, Katsumi Imada   +4 more
openaire   +2 more sources

Green fluorescent protein as a reporter of prion protein folding

Virology Journal, 2006
Background The amino terminal half of the cellular prion protein PrPc is implicated in both the binding of copper ions and the conformational changes that lead to disease but has no defined structure. However, as some structure is likely to exist we have
Dalton Kevin, Yao YongXiu, Ren Junyuan, Vasiljevic Snezana, Adamson Catherine S, Jones Ian M   +5 more
doaj   +1 more source