Guanine Nucleotide Exchange Assay Using Fluorescent MANT-GDP
Bio-Protocol, 2018 GTPases are molecular switches that cycle between the inactive GDP-bound state and the active GTP-bound state. GTPases exchange nucleotides either by its intrinsic nucleotide exchange or by interaction with guanine nucleotide exchange factors (GEFs ...
Tomoharu Kanie, Peter Jackson
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The many faces of the guanine-nucleotide exchange factor trio [PDF]
Cell Adhesion & Migration, 2012 Small Rho-GTPases are enzymes that are bound to GDP or GTP, which determines their inactive or active state, respectively. The exchange of GDP for GTP is catalyzed by so-called Rho-guanine nucleotide exchange factors (GEFs). Rho-GEFs are characterized by a Dbl-homology (DH) and adjacent Pleckstrin-homology (PH) domain that serves as enzymatic unit for ...
van Rijssel, Jos, van Buul, Jaap D.
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Scambio, a novel guanine nucleotide exchange factor for Rho
Molecular Cancer, 2004 Background Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades and mitogenesis.
Groffen John +5 more
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Non-muscle myosin II regulates neuronal actin dynamics by interacting with guanine nucleotide exchange factors. [PDF]
PLoS ONE, 2014 Non-muscle myosin II (NM II) regulates a wide range of cellular functions, including neuronal differentiation, which requires precise spatio-temporal activation of Rho GTPases.
Eun-Young Shin +7 more
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P-Rex2, a new guanine-nucleotide exchange factor for Rac [PDF]
FEBS Letters, 2004 We have identified a new guanine‐nucleotide exchange factor, P‐Rex2, and cloned it from human skeletal muscle and brain libraries. It has widespread tissue distribution but is not expressed in neutrophils. P‐Rex2 is a 183 kDa protein that activates the small GTPase Rac and is regulated by phosphatidylinositol (3,4,5)‐trisphosphate and the βγ subunits ...
Charlotte Lecureuil +2 more
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Structural biology of DOCK-family guanine nucleotide exchange factors. [PDF]
FEBS Lett, 2023 DOCK proteins are a family of multi‐domain guanine nucleotide exchange factors (GEFs) that activate the RHO GTPases CDC42 and RAC1, thereby regulating several RHO GTPase‐dependent cellular processes. DOCK proteins are characterized by the catalytic DHR2
Boland A, Côté JF, Barford D.
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The Guanine Nucleotide Exchange Factor GBF1 Participates in Rotavirus Replication [PDF]
Journal of Virology, 2019 Rotavirus, a member of the family Reoviridae , is the major cause of severe diarrhea in children and young animals worldwide. Despite significant advances in the characterization of the biology of this virus, the mechanisms involved in morphogenesis of the virus particle are still poorly ...
Martínez, José L +9 more
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Comprehensive behavioral analysis of mice deficient in Rapgef2 and Rapgef6, a subfamily of guanine nucleotide exchange factors for Rap small GTPases possessing the Ras/Rap-associating domain [PDF]
Molecular Brain, 2018 Rapgef2 and Rapgef6 define a subfamily of guanine nucleotide exchange factors for Rap small GTPases, characterized by the possession of the Ras/Rap-associating domain.
Kazuhiro Maeta +6 more
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Editorial: Guanine nucleotide exchange factors as determinants of cell fate and development [PDF]
Frontiers in Cell and Developmental BiologyVegesna Radha +4 more
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The RasGrf family of mammalian guanine nucleotide exchange factors
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, 2011 RasGrf1 and RasGrf2 are highly homologous mammalian guanine nucleotide exchange factors which are able to activate specific Ras or Rho GTPases. The RasGrf genes are preferentially expressed in the central nervous system, although specific expression of either locus may also occur elsewhere.
Fernández Medarde, Alberto +1 more
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