Results 91 to 100 of about 104,742 (325)

Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides

Genome Biology, 2017
We report that engineered Cas9 variants with improved specificity—eCas9-1.1 and Cas9-HF1—are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5’ terminus, relative to target DNA sequences.
Sojung Kim, Taegeun Bae, Jaewoong Hwang, Jin-Soo Kim   +3 more
doaj   +1 more source

Homologous and unique G protein alpha subunits in the nematode Caenorhabditis elegans [PDF]

, 1991
A cDNA corresponding to a known G protein alpha subunit, the alpha subunit of Go (Go alpha), was isolated and sequenced. The predicted amino acid sequence of C. elegans Go alpha is 80-87% identical to other Go alpha sequences.
Lochrie, Michael A., Mendel, Jane E., Simon, Melvin I., Sternberg, Paul W.   +3 more
core   +1 more source

Cell‐Free DNA‐Based Theranostics for Inflammatory Disorders

Advanced Science, EarlyView.
Summary on the dual potential of cfDNA as biomarkers and therapeutic targets for inflammatory disorders. Figure was created with BioRender.com. ABSTRACT Inflammatory disorders are characterized by immune‐mediated inflammatory cascades that can affect multiple organs.
Jiatong Li, Yumei Wang, Xiaoxi Fan, Miao Xu, Jiawen Li, Wenfei Tan, Jianxiang Zhang, Heran Li   +7 more
wiley   +1 more source

First-principles GW calculations for DNA and RNA nucleobases

, 2011
On the basis of first-principles GW calculations, we study the quasiparticle properties of the guanine, adenine, cytosine, thymine, and uracil DNA and RNA nucleobases.
A. Travers, Carina Faber, Claudio Attaccalite, E. Runge, H. Lodish, V. Olevano, X. Blase   +6 more
core   +4 more sources

Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif [PDF]

, 2017
Heterotrimeric G proteins are quintessential signalling switches activated by nucleotide exchange on Gα. Although activation is predominantly carried out by G-protein-coupled receptors (GPCRs), non-receptor guanine-nucleotide exchange factors (GEFs) have
Baillie, George S., Blanco, Francisco J., Blanco-Canosa, Juan B., Cerione, Richard A., de la Cruz-Morcillo, Miguel Angel, DiGiacomo, Vincent, Garcia-Marcos, Mikel, Ibáñez de Opakua, Alain, Leyme, Anthony, Marivin, Arthur, Merino, Nekane, Nguyen, Lien T., Parag-Sharma, Kshitij, Ramachandran, Sekar, Villate, Maider   +14 more
core   +1 more source

Phylogenomics, ecomorphological evolution, and historical biogeography in Deuterocohnia (Bromeliaceae: Pitcairnioideae)

American Journal of Botany, EarlyView.
Abstract Premise Species of Deuterocohnia (17 spp.) show extraordinary variation in elevation (0–3900 m a.s.l.) and growth forms, and many have narrow geographic distributions in the west‐central Andes and the Peru‐Chile coast. Previous research using few plastid and nuclear loci failed to produce well‐resolved or supported phylogenies.
Bing Li, Nicole Schütz, Kurt Weising, Georg Zizka, Jacob B. Landis, Thomas J. Givnish   +5 more
wiley   +1 more source

Karakteristik Parsial Gen Sitokrom-C Oksidase Subunit-I Katak Pohon Suaka Marga Satwa Tanjung Peropa Moramo, Sulawesi Tenggara (CHARACTERISTICS OF PARTIAL CYTOCHROME C OXIDASE SUBUNIT I (COI) GENE OF TREE FROG (Polypedates celebensis) IN TANJUNG PEROPA WI

Jurnal Veteriner, 2017
Tree frog (Polypedates celebensis) is an endemic amphibi found in Southeast Sulawesi. Database ofthis species, in particular genetic databases is still very limited.
Suriana Suriana, Nasaruddin Nasaruddin
doaj  

Distinct conformational states enable transglutaminase 2 to promote cancer cell survival versus cell death

Communications Biology
Transglutaminase 2 (TG2) is a GTP-binding, protein-crosslinking enzyme that has been investigated as a therapeutic target for Celiac disease, neurological disorders, and aggressive cancers.
Cody Aplin, Kara A. Zielinski, Suzette Pabit, Deborah Ogunribido, William P. Katt, Lois Pollack, Richard A. Cerione, Shawn K. Milano   +7 more
doaj   +1 more source

Long‐Read Genome Sequencing Establishes Biallelic Pathogenic Variants in DNM1 With Distinct Functional Effects as the Cause of Early Infantile Developmental and Epileptic Encephalopathy

American Journal of Medical Genetics Part A, EarlyView.
ABSTRACT Heterozygous de novo and inherited biallelic pathogenic variants in DNM1 have been reported in association with autosomal dominant (AD) and autosomal recessive (AR) developmental and epileptic encephalopathy, respectively, due to aberrant dynamin function or expression, with each inheritance pattern associated with a different mechanism of ...
Andy Drackley, Merlene Peter, Heba H. Akbari, Jelena Ivanisevic, Alexander Ing, Kelly Regan‐Fendt, Patrick McMullen, Kai Lee Yap   +7 more
wiley   +1 more source

Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs

BioTechniques, 2004
Fluorescent cis-platinum compounds that react with the N7 atom of guanine are useful for labeling nucleic acids in fluorescence hybridization applications.
Anton K. Raap, Marja J.M. van der Burg, Jeroen Knijnenburg, Eric Meershoek, Carla Rosenberg, Joe W. Gray, Joop Wiegant, J. Graeme Hodgson, Hans J. Tanke   +8 more
doaj   +1 more source