Results 31 to 40 of about 150,931 (301)

Crystal structures of the human elongation factor eEFSec suggest a non-canonical mechanism for selenocysteine incorporation

Nature Communications, 2016
Specialized translation elongation factors (eEFSec and SelB) promote selenocysteine incorporation into proteins. Here, the authors report the structure of human eEFSec, examine its interactions with guanine nucleotides, and propose a non-canonical ...
Malgorzata Dobosz-Bartoszek, Mark H. Pinkerton, Zbyszek Otwinowski, Srinivas Chakravarthy, Dieter Söll, Paul R. Copeland, Miljan Simonović   +6 more
doaj   +1 more source

Effective and repeatable chromatographic separation of 5 nucleotides in infant formula milk powder by ion-pair high-performance liquid chromatography–ultraviolet

Journal of Dairy Science, 2022
: A robust method using HPLC-UV was developed to improve the accuracy and repeatability of a quantitative method to detect 5 nucleotides (cytidine-monophosphate, uridine monophosphate, adenosine monophosphate, guanine monophosphate, and inosine ...
Yuanjia Chen, Lin Luo, Peiyan Feng, Baojun Xu, Xiaoqun Wei   +4 more
doaj   +1 more source

An integrated approach to evaluate acetamiprid-induced oxidative damage to tRNA in human cells based on oxidized nucleotide and tRNA profiling

Environment International, 2023
Acetamiprid is poisonous to mammals due to severe acetamiprid-induced oxidative stress that could cause mitochondrial dysfunctions, lipid and protein oxidation, inflammation, apoptosis, and DNA damage.
Hui-Xia Zhang, Dian Yu, Jian-Feng Sun, Ling Zeng, Cai-Yun Wang, Li-Ping Bai, Guo-Yuan Zhu, Zhi-Hong Jiang, Wei Zhang   +8 more
doaj   +1 more source

Chromatin structure and context-dependent sequence features control prime editing efficiency

Frontiers in Genetics, 2023
Prime editing (PE) is a highly versatile CRISPR–Cas9 genome editing technique. The current constructs, however, have variable efficiency and may require laborious experimental optimization.
Somang Kim, Somang Kim, Jimmy B. Yuan, Jimmy B. Yuan, Wendy S. Woods, Destry A. Newton, Pablo Perez-Pinera, Pablo Perez-Pinera, Pablo Perez-Pinera, Pablo Perez-Pinera, Pablo Perez-Pinera, Jun S. Song, Jun S. Song, Jun S. Song, Jun S. Song, Jun S. Song   +15 more
doaj   +1 more source

Guanine nucleotides can activate the insulin-stimulated phosphodiesterase in liver plasma membranes [PDF]

, 1983
The insulin-stimulated cyclic AMP phosphodiesterase from liver plasma membranes is shown to be activated upon incubation with guanine nucleotides in the presence of ATP. The non-hydrolysable analogue of ATP, adenylyl imidodiphosphate failed to substitute
Miles D. Houslay, Heyworth, Clare M., Clare M. Heyworth, Rawal, Sushil, Houslay, Miles D., Sushil Rawal   +5 more
core   +1 more source

Dampening of neurotransmitter action: molecular similarity within the melatonin structure

Endocrine Regulations, 2018
Objectives. Melatonin initiates physiologic and therapeutic responses in various tissues through binding to poorly defined MT receptors regulated by G-proteins and purine nucleotides.
Williams Wynford R.
doaj   +1 more source

Lipocalin‐2 activates hepatic stellate cells and promotes nonalcoholic steatohepatitis in high‐fat diet–fed Ob/Ob mice

Hepatology, EarlyView., 2022
Graphical summary of obesity‐induced NASH progression by LCN2 targeted to HSC activation. Abstract Background and Aims In obesity and type 2 diabetes mellitus, leptin promotes insulin resistance and contributes to the progression of NASH via activation of hepatic stellate cells (HSCs).
Kyung Eun Kim, Jaewoong Lee, Hyun Joo Shin, Eun Ae Jeong, Hye Min Jang, Yu Jeong Ahn, Hyeong Seok An, Jong Youl Lee, Meong Cheol Shin, Soo Kyoung Kim, Won Gi Yoo, Won Ho Kim, Gu Seob Roh   +12 more
wiley   +1 more source

SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome

eLife, 2022
Viral infection involves complex set of events orchestrated by multiple viral proteins. To identify functions of SARS-CoV-2 proteins, we performed transcriptomic analyses of cells expressing individual viral proteins.
Michela Zaffagni, Jenna M Harris, Ines L Patop, Nagarjuna Reddy Pamudurti, Sinead Nguyen, Sebastian Kadener   +5 more
doaj   +1 more source

Guanine nucleotides inhibit agonist-stimulated arachidonic acid release in both intact and saponin-permeabilized human platelets [PDF]

, 1988
The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other ...
Wu, Hung, Stephen P. Halenda, Alan G. Rehm, Halenda, Stephen P., Rehm, Alan G., Hung Wu   +5 more
core   +1 more source

Genetic predisposition to porto‐sinusoidal vascular disorder: A functional genomic‐based, multigenerational family study

Hepatology, EarlyView., 2022
A deleterious variant of FCHSD1 results in mTOR pathway overactivation and may cause porto‐sinusoidal vascular disorder (PSVD). The pedigree of the family demonstrated an autosomal dominant disease with variable expressivity. Whole‐genome sequencing and Sanger sequencing both validated the existence of the FCHSD1 variant and the heterozygosity of c ...
Jingxuan Shan, André Megarbane, Aziz Chouchane, Deepak Karthik, Ramzi Temanni, Atilio Reyes Romero, Huiying Hua, Chun Pan, Xixi Chen, Murugan Subramanian, Chadi Saad, Hamdi Mbarek, Cybel Mehawej, Eliane Chouery, Sirin W. Abuaqel, Alexander Dömling, Sami Remadi, Cesar Yaghi, Pu Li, Lotfi Chouchane   +19 more
wiley   +1 more source