Results 31 to 40 of about 52,163 (306)
SARS-CoV-2 Nsp14 mediates the effects of viral infection on the host cell transcriptome
eLife, 2022 Viral infection involves complex set of events orchestrated by multiple viral proteins. To identify functions of SARS-CoV-2 proteins, we performed transcriptomic analyses of cells expressing individual viral proteins.
Michela Zaffagni +5 more
doaj +1 more source
Mitochondrial metabolism of guanine nucleotides possible role of guanosine
, 1989 The catabolism of intramitochondrial guanine nucleotides was examined. During 30 min incubation of rat liver mitochondria at 37°C in the presence of oligomycin and carboxyatractyloside, guanine and xanthine were formed and appeared in the medium.
Tikhonov, Yuri V. +13 more
core +1 more source
eLife, 2020 We report the in vivo regulation of Inosine-5´-monophosphate dehydrogenase 1 (IMPDH1) in the retina. IMPDH1 catalyzes the rate-limiting step in the de novo synthesis of guanine nucleotides, impacting the cellular pools of GMP, GDP and GTP.
Anna Plana-Bonamaisó +13 more
doaj +1 more source
Nucleotide discrimination with DNA immobilized in the MspA nanopore. [PDF]
PLoS ONE, 2011 Nanopore sequencing has the potential to become a fast and low-cost DNA sequencing platform. An ionic current passing through a small pore would directly map the sequence of single stranded DNA (ssDNA) driven through the constriction.
Elizabeth A Manrao +4 more
doaj +1 more source
Subcellular compartmentation of guanine nucleotides and functional relationships between the adenine and guanine nucleotide systems in isolated hepatocytes [PDF]
FEBS Letters, 1979 The subcellular compartmentation of adenine nucleotides between the mitochondria and the cytosol has been clearly demonstrated by the use of different cell fractionation techniques [ 1,2]. However, data concerning the subcellular distribution of guanine nucleotides are not available.
Kleineke, Jochen +2 more
openaire +2 more sources
LRRK1 and LRRK2 bind guanine nucleotides.
, 2012 (A) Nucleotide competition assays with purified 3xFLAG-LRRK1/2 bound to M2-Flag affinity resin and incubated with a fixed concentration of GTP-α-P33 (10 nM) in the presence of 100 µM of cold nucleotides.
Luigi Bubacco (83582) +13 more
core +1 more source
Chasing Particularities of Guanine- and Cytosine-Rich DNA Strands
Molecules, 2020 By substitution of natural nucleotides by their abasic analogs (i.e., 1′,2′-dideoxyribose phosphate residue) at critically chosen positions within 27-bp DNA constructs originating from the first intron of N-myc gene, we hindered hybridization
Marko Trajkovski, Janez Plavec
doaj +1 more source
Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Protein Catalyzed by Guanine Nucleotide Exchange Factors Rabin8 and GRAB [PDF]
Journal of Biological Chemistry, 2013 Small G-proteins of the Ras superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replacement of GDP by GTP.
Guo, Zhong +3 more
openaire +6 more sources
FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
Trapp Stimulates Guanine Nucleotide Exchange on Ypt1p [PDF]
The Journal of Cell Biology, 2000 TRAPP, a novel complex that resides on early Golgi, mediates the targeting of ER-to-Golgi vesicles to the Golgi apparatus. Previous studies have shown that YPT1, which encodes the small GTP-binding protein that regulates membrane traffic at this stage of the secretory pathway, interacts genetically with BET3 and BET5.
W, Wang, M, Sacher, S, Ferro-Novick
openaire +2 more sources