A thermodynamic paradigm for solution demixing inspired by nuclear transport in living cells [PDF]
Phys. Rev. Lett. 118, 158101 (2017), 2016 Living cells display a remarkable capacity to compartmentalize their functional biochemistry. A particularly fascinating example is the cell nucleus. Exchange of macromolecules between the nucleus and the surrounding cytoplasm does not involve traversing a lipid bilayer membrane.
arxiv +1 more source
Non-invasive force measurement reveals the number of active kinesins on a synaptic vesicle precursor in axonal transport regulated by ARL-8 [PDF]
Physical Chemistry Chemical Physics 20, 3403-3410 (2018), 2018 Kinesin superfamily protein UNC-104, a member of the kinesin-3 family, transports synaptic vesicle precursors (SVPs). In this study, the number of active UNC-104 molecules hauling a single SVP in axons in the worm Caenorhabditis elegans was counted by applying a newly developed non-invasive force measurement technique.
arxiv +1 more source
Microtubule catastrophe from protofilament dynamics [PDF]
Phys. Rev. E 88, 032717 (2013), 2013 The disappearance of the guanosine triphosphate (GTP)-tubulin cap is widely believed to be the forerunner event for the growth-shrinkage transition (`catastrophe') in microtubule filaments in eukaryotic cells. We study a discrete version of a stochastic model of the GTP cap dynamics, originally proposed by Flyvbjerg, Holy and Leibler (Flyvbjerg, Holy ...
arxiv +1 more source
Modeling oscillatory Microtubule--Polymerization [PDF]
, 2002 Polymerization of microtubules is ubiquitous in biological cells and under certain conditions it becomes oscillatory in time. Here simple reaction models are analyzed that capture such oscillations as well as the length distribution of microtubules.
arxiv +1 more source
Journal of Pharmacological Sciences, 2008 In the present study, binding affinities of 5-hydroxytryptamine-4 (5-HT4) ligands for the human 5-HT4d receptor were determined using the agonist [3H]5-HT and the selective 5-HT4 antagonist [3H]GR113,808. We also compared the affinity differences between
Tadayoshi Mikami +5 more
doaj
Stability analysis of a signaling circuit with dual species of GTPase switches [PDF]
arXiv, 2020 GTPases are molecular switches that regulate a wide range of cellular processes, such as organelle biogenesis, position, shape, and signal transduction. These enzymes operate by toggling between an active ("ON") guanosine triphosphate (GTP)-bound state and an inactive ("OFF") guanosine diphosphate (GDP)-bound state; such a toggle is regulated by GEFs ...
arxiv
Shear-Induced Nitric Oxide Production by Endothelial Cells [PDF]
, 2016 We present a biochemical model of the wall shear stress (WSS)-induced activation of endothelial nitric oxide synthase (eNOS) in an endothelial cell (EC). The model includes three key mechanotransducers: mechanosensing ion channels, integrins and G-protein-coupled receptors. The reaction cascade consists of two interconnected parts.
arxiv +1 more source
Observation of a Change in Bend of an RNA Kissing Complex Using the Angular Dependence of Fluorescence Resonance Energy Transfer [PDF]
, 2014 We report on the observation of a change in the bend angle of an RNA kissing complex upon Rop binding using single-molecular-pair FRET. The angular relationship between the dyes, rather than the distance between them, is shown to be responsible for the observed change in energy transfer. It has long been thought that Rop increases the bend angle of the
arxiv +1 more source
Multiphasic interactions between nucleotides and target proteins [PDF]
arXiv, 2016 The nucleotides guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) bind to target proteins to promote bacterial survival (Corrigan et al. 2016). Thus, the binding of the nucleotides to RsgA, a GTPase, inhibits the hydrolysis of GTP.
arxiv
Ligand-Independent GLUT4 Translocation Induced by Guanosine 5'-O-(3-Thiotriphosphate) Involves Tyrosine Phosphorylation [PDF]
, 1998 Tetsuro Haruta
openalex +2 more sources