Neurosignals, 1993 Effects of 10 mumol/l guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), a non-hydrolyzable analog of guanosine 5'-triphosphate, on 2-[125I]iodomelatonin binding were investigated. In the chicken lung, 10 mumol/l GTP gamma S significantly increased (p < 0.05) the equilibrium dissociation constant (Kd) values, but did not affect the maximum number of ...
Song, Y +7 more
openaire +3 more sources
Two G-proteins act in series to control stimulus-secretion coupling in mast cells: use of neomycin to distinguish between G-proteins controlling polyphosphoinositide phosphodiesterase and exocytosis [PDF]
, 1987 Provision of GTP (or other nucleotides capable of acting as ligands for activation of G-proteins) together with Ca2+ (at micromolar concentrations) is both necessary and sufficient to stimulate exocytotic secretion from mast cells permeabilized with ...
Cockcroft, S, Gomperts, BD, Howell, TW
core
Journal of Biological Chemistry, 1991 Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in conjunction with ...
S C, Olson, E P, Bowman, J D, Lambeth
openaire +2 more sources
Journal of Biological Chemistry, 1987 The effect of the addition of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), the GTP analog which activates the inhibitory guanine nucleotide-binding regulatory protein of adenylyl cyclase (Ni), on the pertussis toxin-mediated ADP-ribosylation reaction was studied in detail.
R, Mattera +3 more
openaire +2 more sources
THE P2X7 RECEPTOR OF HUMAN LEUKOCYTES [PDF]
, 2003 Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellular ATP characteristic of the P2X7 receptor. These responses include opening of a cation selective channel/pore which allows
Gu, Baijun
core +1 more source
Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit [PDF]
, 2004 Heterotrimeric G protein alpha (G alpha) subunits possess intrinsic GTPase activity that leads to functional deactivation with a rate constant of approximately 2 min(-1) at 30 degrees C.
Du, Xinlin +5 more
core +1 more source
Factors Affecting Secretion From Electropermeabilized Human Platelets [PDF]
, 1993 Comparative studies on the regulation of secretion fromdense and α-granules of electropermeabilized human plateletsindicated the involvement of three distinct factors, Ca²⁺, proteinkinase C (PKC) and an unidentified GTP-binding protein (GE).
Coorssen, Jens R.
core
How the sperm triggers the fertilisation calcium wave in the sea urchin egg [PDF]
, 1990 Fertilisation is characterised by an increase in cytosolic free calcium (Ca). This crucial event transforms the egg, releasing it from its state of cell cycle arrest and stimulating embryonic development.
Crossley, Ian Brian
core
Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response. [PDF]
, 2016 The Frank-Starling mechanism allows the amount of blood entering the heart from the veins to be precisely matched with the amount pumped out to the arterial circulation.
Boguslavskyi, A +17 more
core
Transition state structures and the roles of catalytic residues in GAP-facilitated GTPase of Ras as elucidated by (18)O kinetic isotope effects [PDF]
, 2009 Ras-catalyzed guanosine 5\u27 triphosphate (GTP) hydrolysis proceeds through a loose transition state as suggested in our previous study of (18)O kinetic isotope effects (KIE) [ Du , X. et al. ( 2004 ) Proc. Natl. Acad. Sci. U.S.A.
Du, Xinlin, Sprang, Stephen R
core +1 more source