Stimulation of phosphatidylinositol 4-phosphate phosphorylation in human placenta membranes by GTPγS [PDF]
, 1986 In human placenta membranes the rate limiting enzyme for PIP2 formation from PI is PIP kinase. GTPγS is shown to activate PIP kinase by increasing Vmax of the enzyme.
Urumow, T., Wieland, O.H.
core +1 more source
Journal of Pharmacological Sciences, 2008 In the present study, binding affinities of 5-hydroxytryptamine-4 (5-HT4) ligands for the human 5-HT4d receptor were determined using the agonist [3H]5-HT and the selective 5-HT4 antagonist [3H]GR113,808. We also compared the affinity differences between
Tadayoshi Mikami +5 more
doaj
Stability analysis of a signaling circuit with dual species of GTPase switches [PDF]
arXiv, 2020 GTPases are molecular switches that regulate a wide range of cellular processes, such as organelle biogenesis, position, shape, and signal transduction. These enzymes operate by toggling between an active ("ON") guanosine triphosphate (GTP)-bound state and an inactive ("OFF") guanosine diphosphate (GDP)-bound state; such a toggle is regulated by GEFs ...
arxiv
Shear-Induced Nitric Oxide Production by Endothelial Cells [PDF]
, 2016 We present a biochemical model of the wall shear stress (WSS)-induced activation of endothelial nitric oxide synthase (eNOS) in an endothelial cell (EC). The model includes three key mechanotransducers: mechanosensing ion channels, integrins and G-protein-coupled receptors. The reaction cascade consists of two interconnected parts.
arxiv +1 more source
Guanine nucleotide-sensitive interaction of a radiolabeled agonist with a phospholipase C-linked P [PDF]
, 1989 Analogs of ATP and ADP produce a guanine nucleotide-dependent activation of phospholipase C in turkey erythrocyte membranes with pharmacological properties consistent with those of a P2y-purinergic receptor (Boyer, J. L., Downes, C. P., and Harden, T.K. (
core +2 more sources
Two G-proteins act in series to control stimulus-secretion coupling in mast cells: use of neomycin to distinguish between G-proteins controlling polyphosphoinositide phosphodiesterase and exocytosis [PDF]
, 1987 Provision of GTP (or other nucleotides capable of acting as ligands for activation of G-proteins) together with Ca2+ (at micromolar concentrations) is both necessary and sufficient to stimulate exocytotic secretion from mast cells permeabilized with ...
Cockcroft, S, Gomperts, BD, Howell, TW
core
Observation of a Change in Bend of an RNA Kissing Complex Using the Angular Dependence of Fluorescence Resonance Energy Transfer [PDF]
, 2014 We report on the observation of a change in the bend angle of an RNA kissing complex upon Rop binding using single-molecular-pair FRET. The angular relationship between the dyes, rather than the distance between them, is shown to be responsible for the observed change in energy transfer. It has long been thought that Rop increases the bend angle of the
arxiv +1 more source
Factors Affecting Secretion From Electropermeabilized Human Platelets [PDF]
, 1993 Comparative studies on the regulation of secretion fromdense and α-granules of electropermeabilized human plateletsindicated the involvement of three distinct factors, Ca²⁺, proteinkinase C (PKC) and an unidentified GTP-binding protein (GE).
Coorssen, Jens R.
core
THE P2X7 RECEPTOR OF HUMAN LEUKOCYTES [PDF]
, 2003 Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellular ATP characteristic of the P2X7 receptor. These responses include opening of a cation selective channel/pore which allows
Gu, Baijun
core +1 more source
Multiphasic interactions between nucleotides and target proteins [PDF]
arXiv, 2016 The nucleotides guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) bind to target proteins to promote bacterial survival (Corrigan et al. 2016). Thus, the binding of the nucleotides to RsgA, a GTPase, inhibits the hydrolysis of GTP.
arxiv