Guanosine pentaphosphate synthetase from Streptomyces antibioticus is also a polynucleotide phosphorylase [PDF]
Journal of Bacteriology, 1996 The gene for the enzyme guanosine pentaphosphate synthetase I (GPSI) from Streptomyces antibioticus has been cloned and sequenced. The cloned gene functioned as a template in the streptomycete coupled transcription-translation system and directed the synthesis of a protein with the properties expected for GPSI.
G H, Jones, M J, Bibb
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Guanosine Tetra- and Pentaphosphate Promote Accumulation of Inorganic Polyphosphate in Escherichia coli [PDF]
Journal of Biological Chemistry, 1997 High levels of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), generated in response to amino acid starvation in Escherichia coli, lead to massive accumulations of inorganic polyphosphate (polyP). Inasmuch as the activities of the principal enzymes that synthesize and degrade polyP fluctuate only slightly, the polyP accumulation
Michael Cashel +2 more
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Guanosine tetra- and pentaphosphate increase antibiotic tolerance by reducing reactive oxygen species production in Vibrio cholerae [PDF]
Journal of Biological Chemistry, 2018 The pathogen Vibrio cholerae is the causative agent of cholera. Emergence of antibiotic-resistant V. cholerae strains is increasing, but the underlying mechanisms remain unclear. Herein, we report that the stringent response regulator and stress alarmone guanosine tetra- and pentaphosphate ((p)ppGpp) significantly contributes to antibiotic tolerance in
Kang-Mu Lee, Sang Sun Yoon
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Guanosine pentaphosphate phosphohydrolase of Escherichia coli is a long-chain exopolyphosphatase. [PDF]
Proceedings of the National Academy of Sciences, 1993 An exopolyphosphatase [exopoly(P)ase; EC 3.6.1.11] activity has recently been purified to homogeneity from a mutant strain of Escherichia coli which lacks the principal exopoly(P)ase. The second exopoly(P)ase has now been identified as guanosine pentaphosphate phosphohydrolase (GPP; EC 3.6.1.40) by three lines of evidence: (i) the sequences of five ...
J D, Keasling, L, Bertsch, A, Kornberg
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Guanosine tetra- and pentaphosphate synthase activity in chloroplasts of a higher plant: association with 70S ribosomes and inhibition by tetracycline [PDF]
Nucleic Acids Research, 2004 Chloroplasts possess bacterial-type systems for transcription and translation. On the basis of the identification of a Chlamydomonas reinhardtii gene encoding a RelA-SpoT homolog (RSH) that catalyzes the synthesis of guanosine tetra- or pentaphosphate [(p)ppGpp], we have previously suggested the operation of stringent control in the chloroplast genetic
Yuzuru Tozawa, Koji Kasai, Kyo Wakasa
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Regulation of bacterial stringent response by an evolutionarily conserved ribosomal protein L11 methylation [PDF]
mBioLysine and arginine methylation is an important regulator of enzyme activity and transcription in eukaryotes. However, little is known about this covalent modification in bacteria. In this work, we investigated the role of methylation in bacteria.
Hanna E. Walukiewicz +11 more
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Ability of modified forms of phenylalanine tRNA to stimulate guanosine pentaphosphate synthesis by the stringent factor-ribosome complex of E. coli [PDF]
Nucleic Acids Research, 1978 tRNA(Phe) of E. coli, modified at its 4-thiouridine ((4)Srd) and 3-(3-amino-3-carboxypropyl)uridine (nbt(3)Urd) residues, was tested for its ability to induce (p)ppGpp synthesis. The (4)Srd residue was derivatized with the p-azido-phenacyl group, cross-linked to Cyd(13), and the borohydride reduction product of the cross-link was prepared.
James Ofengand, J Ofengand
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Adaptation of a fluoroquinolone-sensitive Shigella sonnei to norfloxacin exposure [PDF]
Royal Society Open ScienceShigella causes shigellosis that requires antibiotic treatment in severe cases. Sublethal antibiotic concentrations can promote resistance, but their effect on antibiotic-sensitive bacteria before resistance development is unclear.
Bao Chi Wong +4 more
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Ribosomal synthesis of guanosine tetra- and pentaphosphate with mRNAs of different chain length [PDF]
FEBS Letters, 1977 V A Erdmann
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Controlled Delivery and Light-Induced Release of Magic Spot Nucleotides in Escherichia coli. [PDF]
Angew Chem Int Ed EnglWe synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Popp C +12 more
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