Structural and mechanistic basis for the regulation of the chloroplast signal recognition particle by (p)ppGpp. [PDF]
FEBS LettLHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Zegarra V +7 more
europepmc +2 more sources
The two PPX-GppA homologues from Mycobacterium tuberculosis have distinct biochemical activities. [PDF]
PLoS ONE, 2012 Inorganic polyphosphate (poly-P), guanosine pentaphosphate (pppGpp) and guanosine tetraphosphate (ppGpp) are ubiquitous in bacteria. These molecules play a variety of important physiological roles associated with stress resistance, persistence, and ...
Mei Y Choi +7 more
doaj +1 more source
Effects of Guanosine Tetraphosphate, Guanosine Pentaphosphate, and β-γ Methylenyl-Guanosine Pentaphosphate on Gene Expression of Escherichia coli In Vitro [PDF]
Proceedings of the National Academy of Sciences, 1974 The effects of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), both produced by E. coli , were measured on the activities of several genes in a cell-free system. Gene activity is measured as gene-directed synthesis of biochemically competent protein or transfer RNA ...
H L, Yang +4 more
openaire +2 more sources
The 5' Cap Epitranscriptome and Beyond: Natural and Engineered 5' Cap Modifications for Optimizing mRNA Therapeutics and Functional Studies. [PDF]
ChemMedChem5′ Cap modifications can be introduced via enzymes or chemical synthesis. Natural and non‐natural 5′ cap structures affect mRNA properties, including translation (T), stability (S), and/or immunogenicity (I). Eukaryotic mRNAs made by in vitro transcription have emerged as medical modalities for vaccination and protein replacement therapy. The 5′ cap is
Dahm GC +3 more
europepmc +2 more sources
eLife, 2022 Guanosine pentaphosphate and tetraphosphate (together referred to as ppGpp) are hyperphosphorylated nucleotides found in bacteria and the chloroplasts of plants and algae.
Shanna Romand +12 more
doaj +1 more source
Template‐independent synthesis of guanosine tetra‐ and pentaphosphates on ribosomes [PDF]
FEBS Letters, 1983 It is shown that Escherichia coli ribosomes carrying poly(Lys)‐tRNA can form (p)ppGpp in the presence of stringent factor in the absence of the poly(A) template. Template‐independent synthesis of (p)ppGpp is suppressed by tetracycline and partially decreases if deacylated tRNA is omitted.
Belitsina, Nadezhda V. +2 more
openaire +2 more sources
NirD curtails the stringent response by inhibiting RelA activity in Escherichia coli
eLife, 2021 Bacteria regulate their metabolism to adapt and survive adverse conditions, in particular to stressful downshifts in nutrient availability. These shifts trigger the so-called stringent response, coordinated by the signaling molecules guanosine tetra and ...
Loïc Léger +4 more
doaj +1 more source
Guanosine pentaphosphate and guanosine tetraphosphate accumulation and induction of Myxococcus xanthus fruiting body development [PDF]
Journal of Bacteriology, 1980 Development of multicellular fruiting bodies of Myxococcus xanthus can be induced by limitation of any of a number of different classes of amino acids. Investigated were amino acids that wild-type strains of M. xanthus are unable to synthesize (isoleucine, leucine, and valine), can synthesize at a low rate (phenylalanine), or can normally synthesize at
C, Manoil, D, Kaiser
openaire +2 more sources
PLoS Pathogens, 2021 Bacteria inhabit diverse environmental niches and consequently must modulate their metabolism to adapt to stress. The nucleotide second messengers guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) (collectively referred to as (p ...
N Y Elizabeth Chau +3 more
doaj +1 more source
Recent functional insights into the magic role of (p)ppGpp in growth control
Computational and Structural Biotechnology Journal, 2023 Rapid growth and survival are two key traits that enable bacterial cells to thrive in their natural habitat. The guanosine tetraphosphate and pentaphosphate [(p)ppGpp], also known as “magic spot”, is a key second messenger inside bacterial cells as well ...
Haoyan Mu +5 more
doaj +1 more source