Ability of modified forms of phenylalanine tRNA to stimulate guanosine pentaphosphate synthesis by the stringent factor-ribosome complex of E. coli [PDF]
Nucleic Acids Research, 1978 tRNA(Phe) of E. coli, modified at its 4-thiouridine ((4)Srd) and 3-(3-amino-3-carboxypropyl)uridine (nbt(3)Urd) residues, was tested for its ability to induce (p)ppGpp synthesis. The (4)Srd residue was derivatized with the p-azido-phenacyl group, cross-linked to Cyd(13), and the borohydride reduction product of the cross-link was prepared.
James Ofengand, James Ofengand
exaly +3 more sources
Adaptation of a fluoroquinolone-sensitive Shigella sonnei to norfloxacin exposure [PDF]
Royal Society Open ScienceShigella causes shigellosis that requires antibiotic treatment in severe cases. Sublethal antibiotic concentrations can promote resistance, but their effect on antibiotic-sensitive bacteria before resistance development is unclear.
Bao Chi Wong +4 more
doaj +2 more sources
Life, 2014 Second messengers are intracellular substances regulated by specific external stimuli globally known as first messengers. Cells rely on second messengers to generate rapid responses to environmental changes and the importance of their roles is becoming ...
Marco Agostoni, Beronda L. Montgomery
doaj +2 more sources
Controlled Delivery and Light-Induced Release of Magic Spot Nucleotides in Escherichia coli. [PDF]
Angew Chem Int Ed EnglWe synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Popp C +12 more
europepmc +2 more sources
Structural and mechanistic basis for the regulation of the chloroplast signal recognition particle by (p)ppGpp. [PDF]
FEBS LettLHCPs are transported to the thylakoid membrane via the (cp)SRP pathway. This process involves a transit complex of (cp)SRP43, (cp)SRP54 and LHCP, which interacts with (cp)FtsY and Alb3 at the membrane. GTP hydrolysis by (cp)SRP54 and (cp)FtsY triggers complex dissociation.
Zegarra V +7 more
europepmc +2 more sources
The 5' Cap Epitranscriptome and Beyond: Natural and Engineered 5' Cap Modifications for Optimizing mRNA Therapeutics and Functional Studies. [PDF]
ChemMedChem5′ Cap modifications can be introduced via enzymes or chemical synthesis. Natural and non‐natural 5′ cap structures affect mRNA properties, including translation (T), stability (S), and/or immunogenicity (I). Eukaryotic mRNAs made by in vitro transcription have emerged as medical modalities for vaccination and protein replacement therapy. The 5′ cap is
Dahm GC +3 more
europepmc +2 more sources
Effects of Guanosine Tetraphosphate, Guanosine Pentaphosphate, and β-γ Methylenyl-Guanosine Pentaphosphate on Gene Expression of Escherichia coli In Vitro [PDF]
Proceedings of the National Academy of Sciences, 1974 The effects of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), both produced by E. coli , were measured on the activities of several genes in a cell-free system. Gene activity is measured as gene-directed synthesis of biochemically competent protein or transfer RNA.
H L, Yang +4 more
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eLife, 2022 Guanosine pentaphosphate and tetraphosphate (together referred to as ppGpp) are hyperphosphorylated nucleotides found in bacteria and the chloroplasts of plants and algae.
Shanna Romand +12 more
doaj +1 more source
Template‐independent synthesis of guanosine tetra‐ and pentaphosphates on ribosomes [PDF]
FEBS Letters, 1983 It is shown that Escherichia coli ribosomes carrying poly(Lys)‐tRNA can form (p)ppGpp in the presence of stringent factor in the absence of the poly(A) template. Template‐independent synthesis of (p)ppGpp is suppressed by tetracycline and partially decreases if deacylated tRNA is omitted.
Belitsina, Nadezhda V. +2 more
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Guanosine pentaphosphate and guanosine tetraphosphate accumulation and induction of Myxococcus xanthus fruiting body development [PDF]
Journal of Bacteriology, 1980 Development of multicellular fruiting bodies of Myxococcus xanthus can be induced by limitation of any of a number of different classes of amino acids. Investigated were amino acids that wild-type strains of M. xanthus are unable to synthesize (isoleucine, leucine, and valine), can synthesize at a low rate (phenylalanine), or can normally synthesize at
C, Manoil, D, Kaiser
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