RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
Biomolecules, 2015 The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown.
Merel Derksen +2 more
doaj +1 more source
Massively parallel Cas13 screens reveal principles for guide RNA design
Nature Biotechnology, 2020 Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable specific and robust target gene knockdown without altering the genome.
H. Wessels +5 more
semanticscholar +1 more source
An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]
, 2018 The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J +20 more
core +1 more source
LncRNAs and Chromatin Modifications Pattern m6A Methylation at the Untranslated Regions of mRNAs
Frontiers in Genetics, 2022 New roles for RNA in mediating gene expression are being discovered at an alarming rate. A broad array of pathways control patterning of N6-methyladenosine (m6A) methylation on RNA transcripts.
Lee O. Vaasjo, Lee O. Vaasjo
doaj +1 more source
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites
Bioinform., 2014 Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA ...
Y. Naito +3 more
semanticscholar +1 more source
Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons
Cell, 2020 CRISPR-Cas defense systems have been coopted multiple times in nature for guide RNA-directed transposition by Tn7-like elements. Prototypic Tn7 uses dedicated proteins for two targeting pathways, one targeting a neutral and conserved attachment site in ...
Michael T. Petassi +2 more
semanticscholar +1 more source
DNA editing in DNA/RNA hybrids by adenosine deaminases that act on RNA. [PDF]
, 2017 Adenosine deaminases that act on RNA (ADARs) carry out adenosine (A) to inosine (I) editing reactions with a known requirement for duplex RNA. Here, we show that ADARs also react with DNA/RNA hybrid duplexes.
Beal, Peter A +2 more
core +1 more source
Optimized Plasmid Construction Strategy for Cas9
Cellular Physiology and Biochemistry, 2018 Background/Aims: The target genome editing technology not only plays an important role in basic biology studies but also holds a great promise for potential clinical applications.
Jianyong Xu +5 more
doaj +1 more source
Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
Nature Biotechnology, 2020 Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. However, use of this technology has been limited by reliance on indirect indexing of single-guide RNAs (sgRNAs).
J. Replogle +16 more
semanticscholar +1 more source
MicroRNA-target binding structures mimic microRNA duplex structures in humans. [PDF]
PLoS ONE, 2014 Traditionally, researchers match a microRNA guide strand to mRNA sequences using sequence comparisons to predict its potential target genes. However, many of the predictions can be false positives due to limitations in sequence comparison alone.
Xi Chen, Lu Shen, Hui-Hsien Chou
doaj +1 more source