Results 31 to 40 of about 570,529 (349)
Engineering CRISPR guide RNAs for programmable RNA sensors
Biochemical Society Transactions, 2023 As the most valuable feature of the CRISPR system, the programmability based on Watson–Crick base pairing has been widely exploited in engineering RNA sensors. The base pairing in these systems offers a connection between the RNA of interest and the CRISPR effector, providing a highly specific mechanism for RNA detection both in vivo and in vitro.
Yang Liu, Wei Liu, Baojun Wang
openaire +3 more sources
Cas9 deactivation with photocleavable guide RNAs [PDF]
Molecular Cell, 2021 Precise control of CRISPR-Cas9 would improve its safety and applicability. Controlled CRISPR inhibition is a promising approach but is complicated by separate inhibitor delivery, incomplete deactivation, and slow kinetics. To overcome these obstacles, we engineered photocleavable guide RNAs (pcRNAs) that endow Cas9 nucleases and base editors with a ...
Roger S. Zou +3 more
openaire +2 more sources
RNA processing: Pocket guides to ribosomal RNA [PDF]
Current Biology, 1997 The functional role of a recently identified class of small nucleolar (sno) RNAs has been elucidated: the 'box H/ACA' snoRNAs act as guide RNAs, specifying the position of evolutionarily conserved pseudouridines in ribosomal (r)RNA via an rRNA-snoRNA base-pairing interaction that forms a 'pseudouridine pocket'.
openaire +3 more sources
RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
Biomolecules, 2015 The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown.
Merel Derksen +2 more
doaj +1 more source
MicroRNA-target binding structures mimic microRNA duplex structures in humans. [PDF]
PLoS ONE, 2014 Traditionally, researchers match a microRNA guide strand to mRNA sequences using sequence comparisons to predict its potential target genes. However, many of the predictions can be false positives due to limitations in sequence comparison alone.
Xi Chen, Lu Shen, Hui-Hsien Chou
doaj +1 more source
High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity [PDF]
, 2013 The RNA-programmable Cas9 endonuclease cleaves double-stranded DNA at sites complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify genomes in multiple cells and organisms, demonstrating its potential as a facile genome ...
D Hockemeyer +26 more
core +1 more source
Chemistry & Biology, 2002 RNAi is routinely used to eliminate gene activity for experimental purposes. However, the precise molecular mechanism of RNAi is unknown. Recent papers partially illuminate this mechanism in human cells, advancing the potential application of RNAi toward the treatment of human disease.
Gopalakrishna Ramaswamy, Frank J. Slack
openaire +3 more sources
CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion
BMC Genomics, 2022 Background Recent discovery of the gene editing system - CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) associated proteins (Cas), has resulted in its widespread use for improved understanding of a variety of biological systems ...
Alexander Krohannon +5 more
doaj +1 more source
LncRNAs and Chromatin Modifications Pattern m6A Methylation at the Untranslated Regions of mRNAs
Frontiers in Genetics, 2022 New roles for RNA in mediating gene expression are being discovered at an alarming rate. A broad array of pathways control patterning of N6-methyladenosine (m6A) methylation on RNA transcripts.
Lee O. Vaasjo, Lee O. Vaasjo
doaj +1 more source
An enhanced CRISPR repressor for targeted mammalian gene regulation. [PDF]
, 2018 The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, but inefficiencies in target-gene silencing have limited its utility.
Cecchi, Ryan J +20 more
core +1 more source