Results 41 to 50 of about 25,820 (160)
Staphylococcus aureus DNA gyrase: mechanism and drug targeting [PDF]
, 2012 Increases in Staphylococcus aureus resistance against existing treatment options and the shortage of new antibiotics signals an urgent need for new treatments for the ongoing battle against the development of antibiotic resistance.
Chung, Terence
core +5 more sources
, 1996 DNA gyrase, a type II topoisomerase, catalyses the introduction of negative supercoils into closed-circular DNA, using the energy from ATP hydrolysis. The reaction mechanism involves the breakage of one DNA double strand (the DNA gate) and the passing of
Andrew Philip. Tingey (7674527)
core +5 more sources
Nature Communications, 2021 The mechanism of DNA gyrase inhibitor stabilization of single-strand DNA cleavage breaks by DNA gyrase has been hypothetical. Here, the authors show experimental evidence of the mechanism using a library of inhibitors with improved binding and employ ...
Anja Kolarič +9 more
doaj +1 more source
Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone
Advanced Science, EarlyView.A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad +12 more
wiley +1 more source
Albicidin, a potent DNA gyrase inhibitor with clinical potential
, 2019 The emergence of multiple antibiotic-resistant bacteria is a serious global problem which requires the development of new effective antimicrobial therapeutics.
Saeed Mujahid Hashimi +1 more
core +1 more source
Decrease in gyrase A protein expression in _E. coli_ cells inhibited by antisense ribozymes [PDF]
, 2008 RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. Nucleic acid-based gene interference technologies represent promising strategies for specific inhibition of
Malathi Raghunathan +1 more
core
Artificial Intelligence‐Guided Phenotypic Drug Repurposing Against Streptococcus pneumoniae
Advanced Science, EarlyView.Integrated artificial intelligence (AI) ensembles identify highly potent repurposed drugs against Streptococcus pneumoniae. Prospective virtual screening of 6747 drugs uncovers nine active candidates, including thiostrepton. This drug exhibits low picomolar potency and bypasses established resistance in multidrug‐resistant strains, demonstrating an ...
Joshua S. Fitch +6 more
wiley +1 more source
, 2009 The genes encoding the subunits of DNA topoisomerase IV (parC and parE) and DNA gyrase (gyrA and gyrB) of Streptococcus pneumoniae were cloned and overproduced in Escherichia coli by using the T7promoter-T7 RNA polymerase system.
Balas, Delia +3 more
core +1 more source
The molecular basis of quinolone drug action on DNA gyrase.
, 1996 Quinolones are a clinically-useful class of antibacterial agents known to target DNA gyrase, a bacterial type II topoisomerase. Gyrase is unique among topoisomerases in its ability to introduce negative supercoils into DNA using the energy derived from ...
Susan Elizabeth. Critchlow (7702562)
core +6 more sources
mSphere, 2016 PprA, a radiation-induced Deinococcus-specific protein, was previously shown to be required for cell survival and accurate chromosome segregation after exposure to ionizing radiation. Here, we used an in vivo approach to determine, by shotgun proteomics,
Alice Devigne +7 more
doaj +1 more source