Results 51 to 60 of about 10,208 (189)

Clinical Validation of Digital PCR for Precise m.3243A>G Heteroplasmy Quantification in Early‐Onset Diabetes

open access: yesJournal of Diabetes Research, Volume 2026, Issue 1, 2026.
Background Mitochondrial DNA m.3243A>G variant causes maternally inherited diabetes with deafness. We developed a droplet digital PCR (dPCR) assay to quantify m.3243A>G heteroplasmy and investigated its prevalence in Chinese Han early‐onset diabetes patients.
Xiaomu Kong   +14 more
wiley   +1 more source

Th1 immune responses can be modulated by varying dimethyldioctadecylammonium and distearoyl-sn-glycero-3-phosphocholine content in liposomal adjuvants [PDF]

open access: yes, 2014
OBJECTIVES: Cationic liposomes of dimethyldioctadecylammonium bromide (DDA) combined with trehalose 6,6'-dibehenate (TDB) elicit strong cell-mediated and antibody immune responses; DDA facilitates antigen adsorption and presentation while TDB potentiates
Bramwell, Vincent W.   +4 more
core   +1 more source

Pleistocene Climate Changes and Lineage Diversification of Sphenarium Grasshoppers (Orthoptera: Pyrgomorphidae)

open access: yesEcology and Evolution, Volume 15, Issue 10, October 2025.
We investigate the phylogeographic history and paleodistribution patterns of four closely related grasshopper species of the genus Sphenarium to test the relative impact of Pleistocene climate fluctuations on their diversification. We found that each species responded differently to these fluctuations, but their divergence, distribution, and ...
Salomón Sanabria‐Urbán   +4 more
wiley   +1 more source

Optimisation of a murine splenocyte mycobacterial growth inhibition assay using virulent Mycobacterium tuberculosis

open access: yesScientific Reports, 2017
In the absence of a validated correlate of protection or robust animal models for human tuberculosis, Mycobacterial growth inhibition assays (MGIAs) aim to assess vaccines ability to inhibit mycobacterial growth in-vitro.
Christina Jensen   +4 more
doaj   +1 more source

ESAT-6 (EsxA) and TB10.4 (EsxH) based vaccines for pre- and post-exposure tuberculosis vaccination. [PDF]

open access: yesPLoS ONE, 2013
The ESX systems from Mycobacterium tuberculosis are responsible for the secretion of highly immunogenic proteins of key importance for bacterial survival and growth. The two prototypic proteins, ESAT-6 (EsxA from ESX-1) and TB10.4 (EsxH from ESX-3) share
Truc Hoang   +8 more
doaj   +1 more source

Unstained Blood Smear Analysis: A Review of Rule‐Based, Machine Learning, and Deep Learning Techniques

open access: yesJournal of Biophotonics, Volume 18, Issue 10, October 2025.
Bright‐field images of unstained smears. (1) Sparse erythrocytes allow straightforward intensity‐ or phase‐based segmentation. (2) Overlapping cells blur boundaries, causing over‐ or under‐segmentation and lowering rule‐based accuracy, thus motivating overlap‐aware algorithms for reliable downstream feature extraction and classification. ABSTRACT Blood
Husnu Baris Baydargil, Thomas Bocklitz
wiley   +1 more source

Complex population genetic structure of the bark beetle predator Thanasimus formicarius (L.) (Coleoptera: Cleridae) across its European range

open access: yesAgricultural and Forest Entomology, Volume 27, Issue 3, Page 437-451, August 2025.
Abstract Thanasimus formicarius (L.) (Coleoptera: Cleridae) is an important bark beetle predator and can reduce bark beetle population densities of some of the most severe forest pests in Europe. We analysed the population genetics and phylogeography of T.
Eva Papek   +19 more
wiley   +1 more source

Analysis of gene expression profiles in two spinal cord injury models

open access: yesEuropean Journal of Medical Research, 2022
Objectives To analyze the changes of gene expression at different timepoints after spinal cord injury (SCI) with tenth segment thoracic injury. Methods Two SCI models, the complete paraplegia (H) and Allen’s strike (D) methods were applied to induce SCI ...
Haifeng Yuan   +5 more
doaj   +1 more source

SECURITY PRIORITIES IN ASSESSING THE EFFECTIVENESS OF DOMESTIC ENTERPRISES

open access: yesФінансово-кредитна діяльність: проблеми теорії та практики, 2021
. The article establishes a causal relationship between economic security and efficiency of the enterprise and hypothesizes about the need to expand approaches to analytical evaluation of efficiency, taking into account the security priorities of the ...
L. Hnylytska   +4 more
doaj   +1 more source

A Computational Model Relating Structure and Reactivity in Enantioselective Oxidations of Secondary Alcohols by (−)-Sparteine−Pd^(II) Complexes [PDF]

open access: yes, 2004
The key interactions responsible for the unique reactivity of (−)-sparteine−PdX_2 complexes (X = chloride, acetate) in the enantioselective oxidation of secondary alcohols have been elucidated using quantum mechanics (B3LYP DFT with the PBF polarizable ...
Goddard, William A., III   +3 more
core   +1 more source

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