Results 61 to 70 of about 467,674 (297)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Rsp5/​Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress [PDF]

, 2014
The heat-shock response is a complex cellular program that induces major changes in protein translation, folding and degradation to alleviate toxicity caused by protein misfolding.
Chan, Gerard T., Comyn, Sophie A., Deshaies, Raymond J., Fang, Nancy N., Gsponer, Joerg, Mayor, Thibault, Persaud, Avinash, Rotin, Daniela, Zhu, Mang   +8 more
core   +2 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

CUL-6/cullin ubiquitin ligase-mediated degradation of HSP-90 by intestinal lysosomes promotes thermotolerance

Cell Reports
Summary: Heat shock can be a lethal stressor. Previously, we described a CUL-6/cullin-ring ubiquitin ligase complex in the nematode Caenorhabditis elegans that is induced by intracellular intestinal infection and proteotoxic stress and that promotes ...
Mario Bardan Sarmiento, Spencer S. Gang, Patricija van Oosten-Hawle, Emily R. Troemel   +3 more
doaj   +1 more source

Systemic analysis of heat shock response induced by heat shock and a proteasome inhibitor MG132. [PDF]

PLoS ONE, 2011
The molecular basis of heat shock response (HSR), a cellular defense mechanism against various stresses, is not well understood. In this, the first comprehensive analysis of gene expression changes in response to heat shock and MG132 (a proteasome ...
Hee-Jung Kim, Hye Joon Joo, Yung Hee Kim, Soyeon Ahn, Jun Chang, Kyu-Baek Hwang, Dong-Hee Lee, Kong-Joo Lee   +7 more
doaj   +1 more source

Electro-hyperthermia in Oncology [PDF]

, 2001
Hyperthermia is a rapidly developing treatment method in oncology. The classical effect is based on well-focused energy absorption targeting the malignant tissue.
Szász, András, Szász, Nóra, Szász, Olivér   +2 more
core  

Mdj1p, a novel chaperone of the DnaJ family, is involved in mitochondrial biogenesis and protein folding [PDF]

, 1994
Mdj1p, a novel member of the DnaJ family, is a heat shock protein that is associated with the inner membrane of mitochondria of Saccharomyces cerevisiae.
Amin, Arretz, Atencio, Bankier, Bardwell, Blumberg, Caplan, Caplan, Caplan, Cheng, Chirico, Church, Craig, Daum, Dear, Deshaies, Ellis, Feldheim, Gamer, Georgopoulos, Gibson, Glick, Hartl, Hill, Kang, Kobayashi, Krieg, Laemmli, Langer, Liberek, Luke, Manning-Krieg, Martin, Myers, Neupert, Neupert, Newman, Olson, Ostermann, Pearson, Pelham, Pfanner, Philippsen, Praekelt, Rassow, Riles, Rothblatt, Sadler, Sanger, Scherer, Schröder, Schwarz, Schwarz, Sherman, Sikorski, Silver, Smith, Southern, Stueber, Wickner, Young, Zhang, Zhong, Zylicz   +63 more
core   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

CLINICAL SIGNIFICANCE OF SERUM AND URINARY HEAT SHOCK PROTEIN 70 LEVEL ASSESSMENT IN PATIENTS WITH CHRONIC GLOMERULONEPHRITIS

Alʹmanah Kliničeskoj Mediciny, 2016
The levels of urinary heat shock protein-70 and serum antibodies to the heat shock protein-70 were assessed in 79 patients with different course variants of the chronic glomerulonephritis. The indices studied were higher in patients with an active course
N. V. Chebotaryova, I. N. Bobkova, N. I. Neprintseva, L. V. Kozlovskaya   +3 more
doaj   +1 more source

Heat Shock Proteins and Antioxidant Genes Involved in Heat Combined with Drought Stress Responses in Perennial Rye Grass

Life, 2022
The frequent occurrence of heat and drought stress can severely reduce agricultural production of field crops. In comparison to a single stress, the combination of both heat (H) and drought (D) further reduce plant growth, survival and yield.
Md Atikur Rahman, Jae Hoon Woo, Yowook Song, Sang-Hoon Lee, Md Mahadi Hasan, Md Abul Kalam Azad, Ki-Won Lee   +6 more
doaj   +1 more source