Results 131 to 140 of about 132,829 (164)
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Expression of Recombinant Human α-Glucosidase in HEK293 Cells
Journal of Agricultural and Food ChemistryIn mammals, intestinal α-glucosidase exists as a maltase-glucoamylase complex (MGAM) and a sucrase-isomaltase complex (SI). In this study, we transiently expressed human MGAM and SI in human embryonic kidney 293 (HEK293) cells. At pH 6.0 and 37 °C, the MGAM-expressing HEK293 cells extract (MGE) exhibited maltase, glucoamylase, and isomaltase activities
So Nishimoto +10 more
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Effects of Wnt proteins on cell proliferation and apoptosis in HEK293 cells
Cell Biology International, 2008AbstractWnt proteins and Wnt signalings have been implicated in a variety of development and cell processes, while aberrant activation of Wnt signaling is linked to a range of cancers in many tissues. In this study, we used the HEK293 cell line to investigate the effects of Wnt3a and Wnt5a on proliferation and apoptosis in a serum starvation culture ...
Liwei, Jia +3 more
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Subcellular localization of human neutral ceramidase expressed in HEK293 cells
Biochemical and Biophysical Research Communications, 2005We previously reported that rat and mouse neutral ceramidases were mainly localized to plasma membranes as a type II integral membrane protein and partly detached from the cells via processing of the N-terminal/anchor sequence when expressed in HEK293 cells [M. Tani, H. Iida, M.
Young-ha, Hwang +4 more
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Properties of Xenopus Kv1.10 channels expressed in HEK293 cells
Journal of Neurobiology, 2004AbstractVoltage‐gated K+ channels play important roles in shaping the characteristics of action potentials and electrical activity. In a previous study, we isolated cDNAs encoding several distinct K+ channel isoforms, including a novel isoform (XKv1.10) expressed in Xenopus laevis spinal cord neurons and myocytes.
Mark, Fry +2 more
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Dimethyl sulfoxide effects on hERG channels expressed in HEK293 cells
Journal of Pharmacological and Toxicological Methods, 2006Dimethyl sulfoxide (DMSO) is widely used as a solvent to facilitate formulation of test substances in cell perfusion solutions. However, DMSO concentration in bath (extracellular) solution is usually limited to 0.1-0.3% to avoid DMSO-induced changes in cell morphology and membrane properties due to elevation of osmolality. The purpose of this study was
Xiaoyi, Du +5 more
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PTPN6 regulates the cell-surface expression of TRPM4 channels in HEK293 cells
Pflügers Archiv - European Journal of Physiology, 2018Transient receptor-potential, cation channel, subfamily M, member 4 (TRPM4) channels regulate a variety of physiological and pathological processes; however, their roles as functional channels under diverse conditions remain unclear. In this study, cytosolic protein tyrosine phosphatase non-receptor type 6 (PTPN6) interacted with TRPM4 channels.
Dong Kun Lee +11 more
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Recombinant Production of Glycoengineered Mucins in HEK293-F Cells
Recombinant mucins are attractive polymeric building blocks for new biomaterials, biolubricants, and therapeutics. Advances in glycoengineered host cell systems now enable the recombinant production of mucins with tailored O-glycan side chains, offering new opportunities to tune the functionality of mucins and investigate the biology of specific O ...Ling-Ting, Huang +2 more
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High Throughput Transfection of HEK293 Cells for Transient Protein Production
2018Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection ...
Tia A, Arena +2 more
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Cell and Tissue Biology, 2019
In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while ...
R. A. Pantina +6 more
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In this study, we present a model for visualization of the exosome transfer of p53-GFP protein between cultured mammalian cells. The temporal parameters of the accumulation of fluorescent labeled protein in recipient cells have been analyzed. We employed HEK293 cells transfected with p53∆Y126-GFP plasmid (a GFP clone) as donor of the exosomes, while ...
R. A. Pantina +6 more
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Experiences with HEK293: A Human Cell Line.
PDA journal of pharmaceutical science and technology, 2016The paper describes considerations taken with regard to a recombinant human cell line (HEK293) producing a therapeutic protein. When working with HEK293 cells, besides standard viral safety issues, special precautions should be taken because this cell line is susceptible to infection by numerous viruses.
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