Results 51 to 60 of about 4,203 (178)

Genome‐Wide Codon Reprogramming Enables a Multifactorially Attenuated Influenza Vaccine with Broad Cross‐Protection

Advanced Science, EarlyView.
Genome‐wide codon reprogramming of influenza A virus introduces 1956 synonymous mutations across five segments and elevates CpG content, causing defective NA packaging, loss of NS1 accumulation, and enhanced ZAP‐mediated antiviral responses without affecting RNA or protein synthesis. These mechanisms result in profound attenuation and potent homologous
Yang Wang, Tianxin Ma, Yujiao He, Qinming Li, Kailin Mai, Minying Mo, Chenyang Cao, Jiahui Li, Pei Feng, Jiaojiao Peng, Jing Sun, Weiqi Pan, Zifeng Yang, Ling Chen   +13 more
wiley   +1 more source

mRNA vaccines encoding computationally optimized hemagglutinin elicit protective antibodies against future antigenically drifted H1N1 and H3N2 influenza viruses isolated between 2018-2020

Frontiers in Immunology
BackgroundThe implementation of mRNA vaccines against COVID-19 has successfully validated the safety and efficacy of the platform, while at the same time revealing the potential for their applications against other infectious diseases.
James D. Allen, James D. Allen, James D. Allen, Ted M. Ross, Ted M. Ross, Ted M. Ross, Ted M. Ross   +6 more
doaj   +1 more source

MARPLE: A Proximity‐Triggered CRISPR‐Cas13 Platform for Ultrasensitive Antibody Detection

Advanced Science, EarlyView.
MARPLE is a modular CRISPR‐Cas13 immunoassay that uses antibody‐induced proximity to trigger a toehold‐exchange reaction, releasing an RNA activator for Cas13. This one‐pot, isothermal architecture enables ultrasensitive (femtomolar) and highly specific detection of diverse antibodies, including therapeutic, pathogen‐specific, and cancer‐associated ...
Elena Spezzani, Luca Capelli, Denise Di Lena, Alejandro Chamorro‐Garcia, Rudy Ippodrino, Alessandro Porchetta, Alessandro Bertucci   +6 more
wiley   +1 more source

Monoclonal antibodies detect different forms of influenza virus hemagglutinin during viral penetration and biosynthesis [PDF]

Journal of Virology, 1985
Monoclonal antibodies specific for the influenza virus A/PR/8/34 hemagglutinin (HA) were used to examine the structure of the HA glycoprotein by immunofluorescence techniques during infection of MDCK cells. One antibody (Y8-10C2), shown previously to detect conformational alterations in the HA coinciding with the acid induction of viral fusion activity,
T, Bächi, W, Gerhard, J W, Yewdell
openaire   +2 more sources

Biomimetic Membrane Interface Technologies for Detection and Isolation of CTCs and EVs: Advances and Opportunities in Liquid Biopsy

Advanced Science, EarlyView.
Biomimetic membrane interface engineering constructs functionalized detection platforms by integrating natural cell membranes, synthetic lipids, or hybrid membranes. This strategy effectively reduces background interference and enables efficient target capture and analysis, showing broad applications in circulating tumor cell separation, extracellular ...
Duo Liu, Jie Yu, Jingxue Li, Yixue Chen, Mengle Peng, Yifan Xu, Yongjun Wu, Lihua Ding, Sitian He   +8 more
wiley   +1 more source

The Hemagglutinin of Influenza A Virus Induces Ferroptosis to Facilitate Viral Replication

Advanced Science
AbstractFerroptosis is a novel form of cell death caused by the accumulation of lipid peroxides in an iron‐dependent manner. However, the precise mechanism underlying the exploitation of ferroptosis by influenza A viruses (IAV) remains unclear. The results demonstrate that IAV promotes its own replication through ferritinophagy by sensitizing cells to ...
Aotian Ouyang, Tong Chen, Yi Feng, Jiahui Zou, Shaoyu Tu, Meijun Jiang, Huimin Sun, Hongbo Zhou   +7 more
openaire   +3 more sources

Membralin Assembles a MAN1B1–VCP Complex to Target Foreign Glycoproteins from the Endoplasmic Reticulum to Lysosomes for Degradation

Advanced Science, EarlyView.
This study identifies Membralin as an ER‐phagy receptor that recruits MAN1B1 and VCP to form a selective ERLAD complex. By sensing dense N‐glycan clusters on viral fusion glycoproteins, this ubiquitin‐independent pathway directs SARS‐CoV‐2 spike, Ebola GP, influenza HA, and HIV‐1 Env to lysosomal degradation, thereby limiting viral infectivity ...
Jing Zhang, Xiaoran Lu, Sunan Li, Tao Wang, Iqbal Ahmad, Yong‐Hui Zheng   +5 more
wiley   +1 more source

Inhibition of Influenza A Virus (H1N1) Fusion by Benzenesulfonamide Derivatives Targeting Viral Hemagglutinin

PLoS ONE, 2011
Hemagglutinin (HA) of the influenza virus plays a crucial role in the early stage of the viral life cycle by binding to sialic acid on the surface of host epithelial cells and mediating fusion between virus envelope and endosome membrane for the release of viral genomes into the cytoplasm.
Lei Zhu, Yuhuan Li, Shaohua Li, Haodong Li, Zongxing Qiu, Chichang Lee, Henry Lu, Xianfeng Lin, Rong Zhao, Li Chen, Jim Z Wu, Guozhi Tang, Wengang Yang   +12 more
openaire   +4 more sources

Structural Analysis of the Evolutionary Origins of Influenza Virus Hemagglutinin and Other Viral Lectins [PDF]

Journal of Virology, 2013
ABSTRACT Influenza virus and other viruses use host cell surface sugars as receptors. Here we show that the sugar-binding domains in influenza virus hemagglutinin and other viral lectins share the same structural fold as human galectins (host lectins).
Lang, Chen, Fang, Li
openaire   +2 more sources

Identification of Mannose‐Capped‐Arabinomannan 101‐mer as a Potential Influenza Virus Vaccine Adjuvant

Advanced Science, EarlyView.
Well‐defined and synthetic mannose‐capped arabinomannan 101‐mer from Mycobacterium tuberculosis cell wall was identified as a potent influenza vaccine adjuvant, boosting the antibody response, realizing full protection and showing excellent safety. ABSTRACT Many natural bacterial components as adjuvants can activate the host immune system, but the ...
Yu‐Fang Zhang, Ruihong Xu, Jia Luo, Yuxin Ma, Guozhi Xiao, Ye Liu   +5 more
wiley   +1 more source