Results 111 to 120 of about 3,016 (188)

High Cholesterol Obviates a Prolonged Hemifusion Intermediate in Fast SNARE-Mediated Membrane Fusion [PDF]

, 2015
Cholesterol is essential for exocytosis in secretory cells, but the exact molecular mechanism by which it facilitates exocytosis is largely unknown. Distinguishing contributions from the lateral organization and dynamics of membrane proteins to vesicle ...
Kiessling, Volker, Tamm, Lukas K., Kreutzberger, Alex J.B.   +2 more
core   +1 more source

CLSTN3B promotes lipid droplet maturation and lipid storage in mouse adipocytes

Nature Communications
Interorganelle contacts facilitate material exchanges and sustain the structural and functional integrity of organelles. Lipid droplets (LDs) of adipocytes are responsible for energy storage and mobilization responding to body needs.
Chuanhai Zhang, Mengchen Ye, Kamran Melikov, Dengbao Yang, Goncalo Dias do Vale, Jeffrey McDonald, Kaitlyn Eckert, Mei-Jung Lin, Xing Zeng   +8 more
doaj   +1 more source

Cellular Trafficking of Sn-2 Phosphatidylcholine Prodrugs Studied with Fluorescence Lifetime Imaging and Super-resolution Microscopy

Precision Nanomedicine, 2018
While the in vivo efficacy of Sn-2 phosphatidylcholine prodrugs incorporated into targeted, non-pegylated lipid-encapsulated nanoparticles was demonstrated in prior preclinical studies, the microscopic details of cell prodrug internalization and ...
Dolonchampa Maji, Jin Lu, Pinaki Sarder, Anne H. Schmieder, Grace Cui, Xiaoxia Yang, Dipanjan Pan, Matthew D. Lew, Samuel Achilefu, Gregory M. Lanza   +9 more
doaj   +1 more source

Transition from hemifusion to pore opening is rate limiting for vacuole membrane fusion

, 2005
Fusion pore opening and expansion are considered the most energy-demanding steps in viral fusion. Whether this also applies to soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor (SNARE)- and Rab-dependent fusion events has ...
Andreas Mayer, Christoph Reese, Reese, C., Mayer, A.   +3 more
core   +1 more source

Boundary Lubrication, Hemifusion, and Self-Healing of Binary Saturated and Monounsaturated Phosphatidylcholine Mixtures

, 2019
A wide range of phosphatidylcholine (PC) lipids with different degrees of unsaturation has been identified in the human synovial fluid and on the cartilage surface.
Jacob Klein (471125), Yifeng Cao (292513), Nir Kampf (1290075)   +2 more
core   +1 more source

Divergent HIV-1 Restriction Phenotypes of IFITMs Expressed in Target Cells and Incorporated into Virions

Biomolecules
Interferon-induced transmembrane proteins (IFITMs) are broad-spectrum antiviral factors that restrict the entry of many enveloped viruses, including HIV-1, by modifying host membrane properties and trapping fusion at the hemifusion stage. Beyond blocking
Smita Verma, David Prikryl, Mariana Marin, Ruben M. Markosyan, Andrea Cimarelli, Gregory B. Melikyan   +5 more
doaj   +1 more source

Disruption of liquid/liquid phase separation in asymmetric GUVs prepared by hemifusion

Abstract Model asymmetric bilayers are useful for studying the coupling between lateral and transverse lipid organization. Here, we used calcium-induced hemifusion to create asymmetric giant unilamellar vesicles (aGUVs) for exploring the phase behavior of 16:0-PC/16:1-PC/Cholesterol, a simplified model for the mammalian plasma membrane.
Kristen B. Kennison-Cook, Frederick A. Heberle   +1 more
openaire   +2 more sources

Quantifying interleaflet coupling of phase behavior and observing anti-registered phases in asymmetric lipid bilayers. [PDF]

Biophys J
Kennison-Cook KB, Cooper AM, Heberle FA.
europepmc   +1 more source

A New Method to Prepare Asymmetric Unilamellar Vesicles: Hemifusion [PDF]

Biophysical Journal, 2019
Thais A. Enoki, Gerald W. Feigenson
openaire   +1 more source

Direct Visualization of Large and Protein-Free Hemifusion Diaphragms [PDF]

Biophysical Journal, 2012
Nikolaus, Joerg, Warner, Jason M., O'Shaughnessy, Ben, Herrmann, Andreas   +3 more
openaire   +1 more source