Membrane Binding of α-Synuclein Stimulates Expansion of SNARE-Dependent Fusion Pore [PDF]
Frontiers in Cell and Developmental Biology, 2021 SNARE-dependent membrane fusion is essential for neurotransmitter release at the synapse. Recently, α-synuclein has emerged as an important regulator for membrane fusion. Misfolded α-synuclein oligomers are potent fusion inhibitors. However, the function
Ryan Khounlo +6 more
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Optimal Detection of Fusion Pore Dynamics Using Polarized Total Internal Reflection Fluorescence Microscopy [PDF]
Frontiers in Molecular Biosciences, 2021 The fusion pore is the initial narrow connection that forms between fusing membranes. During vesicular release of hormones or neurotransmitters, the nanometer-sized fusion pore may open-close repeatedly (flicker) before resealing or dilating irreversibly,
Joerg Nikolaus +12 more
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Stabilization of the SNARE Core by Complexin-1 Facilitates Fusion Pore Expansion [PDF]
Frontiers in Molecular Biosciences, 2021 In the neuron, neurotransmitter release is an essential function that must be both consistent and tightly regulated. The continuity of neurotransmitter release is dependent in large part on vesicle recycling. However, the protein factors that dictate the
Josh Pierson, Yeon-Kyun Shin
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Exocytotic fusion pore under stress [PDF]
Cell Stress, 2020 Exocytosis is a universal process of eukaryotic cells, consisting of fusion between the vesicle and the plasma membranes, leading to the formation of a fusion pore, a channel through which vesicle cargo exits into the extracellular space.
Helena Haque Chowdhury, Robert Zorec
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IFITM3 at the viral fusion-pore checkpoint: membrane energetics and entry-route-dependent outcomes [PDF]
Frontiers in ImmunologyInterferon-induced transmembrane proteins (IFITMs) are small interferon-stimulated membrane proteins that can limit viral entry, but their effects depend on the virus, entry route, and experimental context. This Mini Review focuses on IFITM3 at the viral
Luyu Mao +8 more
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ATPase N-ethylmaleimide-sensitive factor mediated calcium dependent fusion pore closure and endocytosis [PDF]
Communications BiologyFor a long time, it remained an unresolved question how the fusion pore is regulated to close after fusion occurs during kiss-and-run. Our study focuses on the physiological function of NSF in modulating single vesicle fusion events in live cells, using ...
Yudong Wang +10 more
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Fusion pore regulation by cAMP/Epac2 controls cargo release during insulin exocytosis [PDF]
eLife, 2019 Regulated exocytosis establishes a narrow fusion pore as initial aqueous connection to the extracellular space, through which small transmitter molecules such as ATP can exit.
Alenka Guček +6 more
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Local PI(4,5)P2 signaling inhibits fusion pore expansion during exocytosis
Cell Reports, 2023 Summary: Phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) is an important signaling phospholipid that is required for regulated exocytosis and some forms of endocytosis.
Muhmmad Omar-Hmeadi +2 more
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Human LAMP1 accelerates Lassa virus fusion and potently promotes fusion pore dilation upon forcing viral fusion with non-endosomal membrane. [PDF]
PLoS Pathogens, 2022 Lassa virus (LASV) cell entry is mediated by the interaction of the virus glycoprotein complex (GPC) with alpha-dystroglycan at the cell surface followed by binding to LAMP1 in late endosomes. However, LAMP1 is not absolutely required for LASV fusion, as
You Zhang +2 more
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Differential SNARE chaperoning by Munc13-1 and Munc18-1 dictates fusion pore fate at the release site [PDF]
Nature CommunicationsThe regulated release of chemical messengers is crucial for cell-to-cell communication; abnormalities in which impact coordinated human body function. During vesicular secretion, multiple SNARE complexes assemble at the release site, leading to fusion ...
Bhavya R. Bhaskar +7 more
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