Results 21 to 30 of about 1,288,108 (273)
Three membrane fusion pore families determine the pathway to pore dilation. [PDF]
Biophys J, 2023 Abstract During exocytosis secretory vesicles fuse with a target membrane and release neurotransmitters, hormones or other bioactive molecules through a membrane fusion pore. The initially small pore may subsequently dilate for full contents release, as commonly observed in amperometric traces.
Su R +3 more
europepmc +4 more sources
Fusion Pore Formation Observed during SNARE-Mediated Vesicle Fusion with Pore-Spanning Membranes. [PDF]
Biophys J, 2020 ABSTRACT Planar pore-spanning membranes (PSMs) have been shown to be a versatile tool to resolve docking and elementary steps of the fusion process with single large unilamellar vesicles (LUVs). However, in previous studies, we monitored only lipid mixing and did not gather information about the formation of fusion pores.
Mühlenbrock P +4 more
europepmc +5 more sources
The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores
eLife, 2021 All membrane fusion reactions proceed through an initial fusion pore, including calcium-triggered release of neurotransmitters and hormones. Expansion of this small pore to release cargo is energetically costly and regulated by cells, but the mechanisms ...
Zhenyong Wu +5 more
doaj +1 more source
Frontiers in Cell and Developmental Biology, 2021 Impaired insulin release is a hallmark of type 2 diabetes and is closely related to chronically elevated glucose concentrations, known as “glucotoxicity.” However, the molecular mechanisms by which glucotoxicity impairs insulin secretion remain poorly ...
Guoyi Yang +9 more
doaj +1 more source
Artificial stabilization of the fusion pore by intra-organelle styrene-maleic acid copolymers [PDF]
, 2021 Styrene-maleic acid copolymers have become an advantageous detergent-free alternative for membrane protein isolation. Since their discovery, experimental membrane protein extraction and purification by keeping intact their lipid environment has become ...
Marcelo, Caparotta +2 more
core +2 more sources
α-Synuclein is required for sperm exocytosis at a post-fusion stage
Frontiers in Cell and Developmental Biology, 2023 The sperm acrosome is a large dense-core granule whose contents are secreted by regulated exocytosis at fertilization through the opening of numerous fusion pores between the acrosomal and plasma membranes. In other cells, the nascent pore generated when
Micaela Vanina Buzzatto +9 more
doaj +1 more source
Core size effects of laser fusion subcritical research reactor for fusion engineering research [PDF]
, 2021 A multi-purpose high repetition laser facility, the so-called Japan establishment for power-laser community harvest (J-EPoCH) is proposed as a next generation laser facility. J-EPoCH will operate at the maximum rate of 100 Hz.
Kodama, Ryousuke, Iwamoto, Akifumi
core +1 more source
Structure and Composition of the Fusion Pore [PDF]
Biophysical Journal, 2003 Earlier studies using atomic force microscopy (AFM) demonstrated the presence of fusion pores at the cell plasma membrane in a number of live secretory cells, revealing their morphology and dynamics at nm resolution and in real time. Fusion pores were stable structures at the cell plasma membrane where secretory vesicles dock and fuse to release ...
Jena, Bhanu P. +4 more
openaire +2 more sources
eLife, 2020 Vesicle fusion is mediated by assembly of SNARE proteins between opposing membranes. While previous work suggested an active role of SNARE transmembrane domains (TMDs) in promoting membrane merger (Dhara et al., 2016), the underlying mechanism remained ...
Madhurima Dhara +5 more
doaj +1 more source
The Journal of Physical Chemistry Letters, 2020 Biological transmission of vesicular content occurs by opening of a fusion pore. Recent experimental observations have illustrated that fusion pores between vesicles that are docked by an extended flat contact zone are located at the edge (vertex) of this zone. We modeled this experimentally observed scenario by coarse-grained molecular simulations and
Blokhuis, Edgar M +3 more
openaire +8 more sources