Results 11 to 20 of about 1,811,201 (303)
Exocytotic fusion pore under stress [PDF]
Cell Stress, 2020 Exocytosis is a universal process of eukaryotic cells, consisting of fusion between the vesicle and the plasma membranes, leading to the formation of a fusion pore, a channel through which vesicle cargo exits into the extracellular space.
Helena Haque Chowdhury, Robert Zorec
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Fusion Pore Formation Observed during SNARE-Mediated Vesicle Fusion with Pore-Spanning Membranes. [PDF]
Biophys J, 2020 Planar pore-spanning membranes (PSMs) have been shown to be a versatile tool to resolve docking and elementary steps of the fusion process with single large unilamellar vesicles (LUVs). However, in previous studies, we monitored only lipid mixing and did
Mühlenbrock P +4 more
europepmc +6 more sources
Dynamics and number of trans-SNARE complexes determine nascent fusion pore properties. [PDF]
Nature, 2018 The fusion pore is the first crucial intermediate formed during exocytosis, yet little is known about the mechanisms that determine the size and kinetic properties of these transient structures.
Bao H +9 more
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α-Synuclein promotes dilation of the exocytotic fusion pore. [PDF]
Nat Neurosci, 2017 The protein α-synuclein has a central role in the pathogenesis of Parkinson's disease. Like that of other proteins that accumulate in neurodegenerative disease, however, the function of α-synuclein remains unknown.
Logan T +5 more
europepmc +4 more sources
Vesicle cholesterol controls exocytotic fusion pore. [PDF]
SSRN Electronic Journal, 2021 In some lysosomal storage diseases (LSD) cholesterol accumulates in vesicles. Whether increased vesicle cholesterol affects vesicle fusion with the plasmalemma, where the fusion pore, a channel between the vesicle lumen and the extracellular space, is ...
Boštjan Rituper +17 more
semanticscholar +5 more sources
The mystery of the fusion pore. [PDF]
Nat Struct Mol Biol, 2016 Release of neurotransmitters occurs by opening of a fusion pore in a manner thought to be mediated by SNARE proteins, but whether the fusion pore is a lipidic or a proteinaceous structure is controversial. A new study using very small nanodiscs shows that it is both.
Sharma S, Lindau M.
europepmc +6 more sources
Fusion Pore Expansion and Contraction during Catecholamine Release from Endocrine Cells. [PDF]
Biophys J, 2020 Amperometry recording reveals the exocytosis of catecholamine from individual vesicles as a sequential process, typically beginning slowly with a pre-spike foot, accelerating sharply to initiate a spike, reaching a peak, and then decaying.
Jackson MB, Hsiao YT, Chang CW.
europepmc +2 more sources
ATPase N-ethylmaleimide-sensitive factor mediated calcium dependent fusion pore closure and endocytosis [PDF]
Communications BiologyFor a long time, it remained an unresolved question how the fusion pore is regulated to close after fusion occurs during kiss-and-run. Our study focuses on the physiological function of NSF in modulating single vesicle fusion events in live cells, using ...
Yudong Wang +10 more
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The fusion pore, 60 years after the first cartoon. [PDF]
FEBS Lett, 2018 Neurotransmitter release occurs in the form of quantal events by fusion of secretory vesicles with the plasma membrane, and begins with the formation of a fusion pore that has a conductance similar to that of a large ion channel or gap junction.
Sharma S, Lindau M.
europepmc +2 more sources
Molecular mechanism of fusion pore formation driven by the neuronal SNARE complex. [PDF]
Proc Natl Acad Sci U S A, 2018 Significance SNARE proteins facilitate the synaptic vesicle fusion with the plasma membrane. The structure of the fusion pore and the mechanism of its formation are not clearly understood. Using coarse-grained molecular dynamics simulations of a nanodisc
Sharma S, Lindau M.
europepmc +2 more sources